استفاده از نانوکپسول های حامل آستاگزانتین میکروجلبک هماتوکوکوس با پوشش مالتودکسترین-کازئینات سدیم به عنوان جایگزین نیتریت سدیم در فرمولاسیون سوسیس معمولی و ارزیابی خصوصیات میکروبی و بافتی محصول

نویسندگان
سکینه یگانه 1
سهیل ریحانی پول 2
1 استاد، گروه شیلات، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
2 دانش آموخته دکتری تخصصی، گروه فراوری محصولات شیلاتی، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
10.22034/FSCT.19.131.143
چکیده
در تحقیق حاضر پس از استخراج آستاگزانتین از میکروجلبک هماتوکوکوس (Haematococcus pluvialis) و نانو ریزپوشانی آن با پوشش ترکیبی مالتودکسترین-کازئینات سدیم، نانوکپسول­های حامل با نسبت­های مختلف، جایگزین نیتریت سدیم (حد مجاز mg/kg120) در فرمولاسیون سوسیس معمولی شدند. سپس خصوصیات میکروبی (شمارش باکتری­های مزوفیل، سرماگرا، انتروباکتریاسه، لاکتیک­اسید و سودوموناس) و بافتی سوسیس­های فرموله­شده طی دوره نگهداری (28 روز در دمای یخچال) مورد ارزیابی و مقایسه قرار گرفتند. نتایج نشان داد تیمارهایی که با نسبت­های 1 (mg/kg30) به 3 (mg/kg90) و 1 (mg/kg60) به 1 (mg/kg60) نانوکپسول به نیتریت سدیم فرموله شده بودند (C و D)، در مقایسه با تیمار داری 120 میلی­گرم بر کیلوگرم نیتریت سدیم (A)، کارائی یکسانی از نظر توانایی مهار رشد و تکثیر گروه­های باکتریایی دارند (05/0<p) و در کل دوره نگهداری، حداقل شمارش باکتری­ها مربوط به این تیمارها است (05/0>p). تیمار حاوی 90 میلی­گرم بر کیلوگرم نانوکپسول­ و 30 میلی­گرم بر کیلوگرم نیتریت سدیم (E) در مهار برخی از گروه­های باکتریایی تا اواسط دوره نگهداری قابلیتی در حد تیمارهای A، C و D داشت (05/0<p). همچنین سطح شمارش در تیمار صرفا حاوی نانوکپسول­ (mg/kg120، B)، به صورت قابل ملاحظه­ای کمتر از شاهد بود (05/0>p). نتایج بخش سنجش خصوصیات بافتی تیمارها نشان داد که اثر نانوکپسول­های حامل آستاگزانتین بر افزایش ظرفیت نگهداری آب سوسیس­ها و همچنین کاهش افت پخت، سختی، صمغیت، قابلیت جویدن و برش بافت بیشتر از نیتریت­ سدیم است (05/0>p). شاخص­های قابلیت ارتجاعی، پیوستگی میان­بافتی و تخلخل سوسیس­های فرموله­شده با نسبت­های مختلف نانوکپسول و نیتریت سدیم فاقد اختلاف معنی­دار (05/0<p) و مطلوب­تر از شاهد بودند (05/0>p). در ادامه مشخص شد که شاخص­های بافتی تیمارهای فرموله­شده طی دوره نگهداری (بر خلاف شاهد) تغییر معنی­داری نداشتند (05/0<p). بنابر یافته­ها، نانوکپسول­های حامل آستاگزانتین پتانسیل بالقوه­ای برای جایگزینی نیتریت سدیم در فرمولاسیون سوسیس به منظور بهبود خصوصیات میکروبی و بافتی دارند.
کلیدواژه‌ها
آستاگزانتین
نانوکپسول های حامل
نیتریت سدیم
سوسیس
خصوصیات میکروبی و بافتی

موضوعات

عنوان مقاله English

Use of nanocapsules carrying astaxanthin from Haematococcus microalgae coated by maltodextrin-sodium caseinate as a substitute for sodium nitrite in formulation of common sausage and evaluating microbial and texture properties of the product

نویسندگان English

Sakineh Yeganeh 1
Soheyl Reyhani Poul 2
1 Professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 PhD graduate, Department of Processing of Fishery Products, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده English

In the present research, after extracting astaxanthin from Haematococcus microalgae and nanoencapsulation it with maltodextrin-sodium caseinate combination coating, carrier nanocapsules with different ratios replaced sodium nitrite (limit of 120 mg/kg) in the sausage formulation. Then, the microbial (Count of mesophilic, psychrophilic, enterobacteriaceae, lactic acid and pseudomonas bacteria) and tissue properties of the formulated sausages were evaluated and compared during the storage period (28 days at refrigerator temperature). The results showed that the treatments that were formulated with ratios of 1 (30 mg/kg) to 3 (90 mg/kg) and 1 (60 mg/kg) to 1 (60 mg/kg) of nanocapsule to sodium nitrite (C and D) have the same efficiency in terms of the ability to inhibit the growth and proliferation of bacterial groups compared to the treatment of 120 mg/kg (A) of sodium nitrite and in the all of storage period, the minimum count of bacteria is related to these treatments. The treatment containing 90 mg/kg of nanocapsules and 30 mg/kg of sodium nitrite (E) had the same ability as treatments A, C and D in inhibiting some bacterial groups until the middle of the storage period. Also, the count level in the treatment containing only nanocapsules (120 mg/kg, B) was significantly lower than the control. The results of measuring the texture characteristics of the treatments showed that the effect of nanocapsules carrying astaxanthin on increasing the water holding capacity of sausages and also reducing of cooking loss, hardness, gumminess, chewiness and tissue cutting is more than sodium nitrite. Springiness, cohesiveness and porosity indices of sausages formulated with different proportions of nanocapsules and sodium nitrite had no significant difference (p>0.05) and were more favorable than the control. In the following, it was found that the texture indicators of the formulated treatments (unlike the control) did not change significantly during the storage period (p>0.05).

کلیدواژه‌ها English

astaxanthin
Carrier nanocapsules
Sodium nitrite
Sausages
Microbial and tissue characteristics
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