بهینه سازی استخراج موسیلاژ از دانه میوه "به" به کمک مایکروویو و با استفاده از روش سطح پاسخ

نویسندگان
1 دانشگاه علوم کشاورزی و منابع طبیعی خوزستان
2 2- استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
3 3- دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده
صمغهای غذایی از جمله افزودنیهایی هستند که نقشهای گوناگونی از قبیل قوام دهندگی، ژل دهندگی و پایدارکنندگی را در محلولهای غذایی ایفا میکنند. در این پژوهش بهترین شرایط استخراج صمغ دانه "به" و همچنین خصوصیات آنتی اکسیدانی و فیزیکو شیمیایی آن مورد بررسی قرار گرفت. راندمان استخراج صمغ دانه به، با استفاده از متغیرهای توان مایکروویو (850-250 وات)، نسبت آب به دانه (20-60) و زمان استخراج (9-2 دقیقه)، با روش سطح پاسخ بهینه سازی شد. خصوصیات فیزیکوشیمیایی صمغ شامل خاکستر، رطوبت، پروتئین و چربی اندازه گیری و خواص آنتی اکسیدانی با روش DPPH ، ABTS، FRAP و آزمون بی رنگ شدن بتاکاروتن ارزیابی گردید. بازدهی استخراج در دامنه %9/8 تا 2%/15 بود. با افزایش توان مایکروویو در زمان ثابت، بازده استخراج صمغ افزایش می یابد، لیکن افزایش توان هم زمان با افزایش زمان تیمار منجر به کاهش میزان استخراج صمغ گردید. افزایش زمان همزمان با ثابت نگه داشتن توان در ابتدا منجر به افزایش استخراج و پس از آن منجر به کاهش میزان حصول صمغ شد. قدرت مهار کنندگی و خاصیت آنتی اکسیدانی با روشهای DPPH، FRAP، ABTS و آزمون بی رنگ شدن بتاکاروتن ، به ترتیب 19/53 میکروگرم بر میلی لیتر، 06/1 میکروگرم بر میلی لیتر، 61/69 میکروگرم برمیلی لیتر، 92/2 میلی گرم در 100 گرم، میزان فنل و فلاونوئید کل 66/74 میلی گرم معادل اسید گالیک در گرم نمونه و 21/670 میلی گرم کوئرستین بر گرم خشک نمونه به دست آمد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Optimization of Microwave Extraction of Quince Seed mucilage using Response Surface Methodology

نویسندگان English

Bahareh Goodarzi ShamsAbadi 1
Mohammad Amin Mehrnia 2
Mohammad Noshad 3
Behrooz Alizadeh Behbahani 2
Hassan Barzegar 3
1 Agricultural sciences and natural resources of Khuzestan
2 Agricultural sciences and natural resources university of Khuzestan
3 Agricultural sciences and natural resources university of khuzestan
چکیده English

Food gums are additives that play variety of roles such as consistency, gel formation, and stabilizing in food solutions. In this study, the best conditions for extracting Quince seed gum as well as its antioxidant and physicochemical properties were investigated. Extraction of gum was optimized using microwave power (250 -850 W), water to grain ratio (20-60) and time (2-9 min), by the response surface methodology. Gum physicochemical properties including ash, moisture, protein and fat were measured and antioxidant properties were evaluated by DPPH, ABTS, FRAP and β-carotene/linoleic acid bleaching assay. The extraction efficiency was in the range of 8.9% to 15.2%. As the microwave power increases in constant time, gum extraction efficiency increases, but the simultaneous power increases with increasing treatment time, resulted in decreasing gum extraction. Increasing time by keeping the power constant, initially resulted in an increase in extraction and afterward resulted in lower gum extraction. Results of scavenging capacity and antioxidant properties measured using DPPH, FRAP, ABTS and beta –carotene bleaching assay were 53.19 μg/ml, 1.06 μg/ml, 69.61 μg/ml, 2.92 μg/100g respectively, and the amount of total phenol and flavonoids were 74.66 mg GAE/g and 670.21mg QE/g respectively.

کلیدواژه‌ها English

Microwave extraction
Quince seed
response surface methodology
Antioxidant capacity
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