بررسی مهارکنندگی و کشندگی قارچ‌های عامل کپک‌زدگی میوه پرتقال (پنی‌سیلیوم دیجیتاتوم و پنی‌سیلیوم ایتالیکوم) با استفاده از عصاره هیدروالکی گیاه اشنان

نویسندگان
مصطفی رحمتی جنیدآباد 1
محمد نوشاد 2
1 استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.52547/fsct.19.124.127
چکیده
در مطالعه حاضر، عصاره هیدروالکلی گیاه اشنان (Seidlitzia rosmarinus) با استفاده از روش خیساندن استخراج گردید و اثر ضد قارچی آن بر علیه قارچ‌های عامل کپک‌زدگی میوه پرتقال (پنی­سیلیوم دیجیتاتوم و پنی­سیلیوم ایتالیکوم) مطابق آزمون‌های دیسک دیفیوژن آگار، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی مورد بررسی قرار گرفت. علاوه بر این، میزان فنول و فلاونوئید تام و فعالیت آنتی‌اکسیدانی عصاره هیدروالکلی گیاه اشنان تعیین گردید. مطابق نتایج آزمون‌های دیسک دیفیوژن و چاهک آگار، فعالیت ضد قارچی عصاره وابسته به غلظت بود و پنی‌سیلیوم دیجیتاتوم حساس‌ترین سویه قارچی در برابر عصاره بود. بعلاوه، هر دو سویه قارچی در حضور 32 میلی‌گرم در میلی‌لیتر عصاره قادر به رشد نبودند و حداقل غلظت کشندگی برای پنی‌سیلیوم دیجیتاتوم برابر با 512 میلی‌گرم در میلی‌لیتر بود. محتوای فنول تام و فلاونوئید تام عصاره به ترتیب برابر با mg GAE/g 19/26 و mg QE/g 10/11 بود. نتایج فعالیت آنتی‌اکسیدانی نشان داد عصاره هیدروالکلی گیاه اشنان قادر به مهار رادیکال‌های آزاد DPPH (14/112 میکروگرم در میلی‌لیتر) و ABTS (46/103 میکروگرم در میلی‌لیتر) می‌باشد. بطور کلی، نتایج این مطالعه نشان می‌دهد که عصاره هیدروالکلی گیاه اشنان حاوی ترکیبات فنولی با قابلیت ضد قارچی و آنتی‌اکسیدانی بالقوه می‌باشد.
کلیدواژه‌ها
گیاه اشنان
عصاره هیدروالکلی
فعالیت ضد قارچی
اثر آنتی‌اکسیدانی

موضوعات

عنوان مقاله English

Inhibitory and lethality effect of Seidlitzia rosmarinus hydroalcoholic extract on mold fungi causing orange fruit rot (Penicillium digitatum and Penicillium italicum)

نویسندگان English

Mostafa Rahmati-Joneidabad 1
Mohammad Noshad 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

In the present study, the hydroalcoholic extract of Seidlitzia rosmarinus was obtained using maceration method and its antifungal effect against fungi species causing orange fruit rot (Penicillium digitatum and Penicillium italicum) according to disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum fungicidal concentration were evaluated. In addition, the content of total phenols and flavonoids and the antioxidant activity of the hydroalcoholic extract of S. rosmarinus were determined. According to the results of disk diffusion and well diffusion agar tests, the antifungal activity of the extract was dependent on its concentration and P. digitatum was the most sensitive fungal strain to the extract. Moreover, both fungal strains were unable to grow in the presence of 32 mg/ml extract and the minimum fungicidal concentration for P. digitatum was 512 mg/ml. The contents of total phenols and total flavonoids of the extract were 26.19 mg GAE/g and 11.10 mg QE/g, respectively. The results of antioxidant activity showed that the hydroalcoholic extract of S. rosmarinus is able to scavenge DPPH (112.14 μg/ml) and ABTS (103.46 μg/ml) free radicals. In general, the results of this study show that the hydroalcoholic extract of S. rosmarinus plant contains phenolic compounds with antifungal and antioxidant potential.

کلیدواژه‌ها English

Seidlitzia rosmarinus
Hydroalcoholic extract
Antifungal activity
Antioxidant effect
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