ویژگی های ضد اکسایشی و ممانعت کنندگی آنزیم آلفا-آمیلاز و آلفا-گلوکوزیداز پلی ساکاریدهای سولفاته خالص سازی شده از گیاه آب شیرین Myriophyllum spicatum L.

نویسندگان
سیده مریم علوی 1
مهدی طبرسا 2
حسن احمدی گاولیقی 3
1 دانش آموخته کارشناسی ارشد، گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران.
2 دانشیار، گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران.
3 دانشیار، گروه صنایع غذایی، دانشکده کشاورزی، دانشگاه تربیت مدرس، نور، ایران.
10.52547/fsct.18.116.81
چکیده
پلی ساکاریدهای سولفاته موجود در دیواره سلولی جلبک‌ها و گیاهان آبزی به دلیل ساختار ویژه شیمیایی و مولکولی از فعالیت های زیستی متنوعی برخوردار هستند. هدف از مطالعه حاضر، ارزیابی پتانسیل پلی ساکاریدهای سولفاته گیاه آبزیMyriophyllum spicatum L. در جلوگیری از واکنش‌های اکسایشی و فعالیت آنزیم‌های گوارشی آلفا-آمیلاز و آلفا-گلوکوزیداز هست. پس از حذف رنگ‌دانه‌ها و ترکیبات با وزن مولکولی پایین، پلی­‌ساکارید­ها توسط آب مقطر در دمای 60 درجه سانتیگراد به مدت دو ساعت استخراج شدند. تخلیص پلی­ساکارید­ها بر روی ستون حاوی ژل DEAE Sepharose FF منجر به تولید دو فراکسیون F1 و F2 با بارالکتریکی متفاوت شد. پلی ساکاریدهای به‌دست‌آمده به میزان قابل‌توجهی قادر به مهار رادیکال‌های آزاد DPPH (62/20-81/64 درصد) و احیاء یون Fe3+ (جذب 24/0-50/0) با بیشترین فعالیت در فراکسیون F1 بودند. بازدارندگی پلی ساکاریدهای بر فعالیت آنزیم آلفا-آمیلاز متفاوت (50/22-20/63 درصد) بود و کمترین IC50 برابر با 31/1 میلی‌گرم بر میلی‌لیتر در فراکسیون F2 مشاهده شد. فعالیت آنزیم آلفا-گلوکوزیداز مخمری نیز توسط پلی ساکاریدها در سطوح مختلفی نسبت به کنترل کاهش یافت (30/1-5/89 درصد) و کمترین IC50 برابر با 0/10 میلی‌گرم بر میلی‌لیتر در پلی سا کارید خام مشاهده شد. به‌طورکلی، نتایج نشان داد که بسته به ساختار شیمیایی و مولکولی پلی ساکاریدهای سولفاته M. spicatum L. دارای پتانسیل ضد اکسایشی و بازدارندگی آنزیم‌های آلفا-آمیلاز و آلفا-گلوکوزیداز بوده و می‌توانند به‌عنوان ترکیبات ضد اکسایشی و ضد دیابتی موردتوجه قرار گیرند.
کلیدواژه‌ها
پلی ساکارید سولفاته
Myriophyllum spicatum
ضداکسایشی
ضد دیابتی

موضوعات

عنوان مقاله English

Antioxidant activity, α-amylase and α-glucosidase inhibition properties of sulfated-polysaccharides purified from freshwater plant Myriophyllum spicatum L.

نویسندگان English

Maryam Alavi 1
Tabarsa Mehdi 2
Hassan Ahmadi Gavlighi 3
1 Tarbiat Modares University
2 Tarbiat Modares University
3 Tarbiat Modares University
چکیده English

Sulfated polysaccharides presenting in the cell wall of seaweeds and aquatic plants exhibit a diverse range of biological functions mainly due to their particular structural and molecular properties. The aim of the current study was to evaluate the inhibition of oxidation reactions and activities of digestive enzymes including α-amylase and α-glucosidase by sulfated polysaccharides from aquatic plant Myriophyllum spicatum. After the removal of pigments and low molecular weight compounds, polysaccharides were extracted by distilled water at 60°C for 2 hours. Polysaccharide purification using DEAE Sepharose FF led to production of two fractions including F1 and F2. Obtained polysaccharides were considerably capable of scavenging DPPH free radicals (20.62-64.81%) and reducing Fe3+ ions (0.24-0.50 Abs) with F1 fraction exhibiting the greatest activity. Polysaccharides inhibitions against α-amylase activity were greatly different (22.50-63.20%) with the lowest IC50 being 1.31 mg/mL for F2 fraction. α-glucosidase activity was reduced to various levels (1.30-89.5%) and the lowest IC50 of 10.0 mg/mL was observed for crude polysaccharide. Overall, these findings indicated that sulfated polysaccharides from M. spicatum L. possess promising antioxidant, α-amylase and α-glucosidase inhibition potentials depending on their chemical and molecular structure and thus could be considered as antioxidant and anti-diabetic agents.

کلیدواژه‌ها English

Sulfated polysaccharides
Myriophyllum spicatum
Antioxidant
Anti-diabetic
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