مقایسه ترکیبات فنولی و فعالیت ضد اکسایشی دو رقم انگور رشه و قزل اوزوم

نویسندگان
لعیا رضازاد باری 1
علیرضا قنبری 2
رضا درویش زاده 3
موسی ترابی گیگلو 4
حامد دولتی بانه 5
1 دانشجوی دکتری تخصصی فیزیولوژی و اصلاح درختان میوه، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
2 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
3 استاد، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران.
4 استادیار، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
5 دانشیار، مرکز تحقیقات و آموزش جهاد کشاورزی استان آذربایجان غربی، ارومیه، ایران.
10.52547/fsct.18.111.1
چکیده
امروزه استفاده از تکنیک کشت بافت در تولید متابولیت­های ثانویه گیاهی، مانند ترکیبات فنولیک، به عنوان افزودنی غذایی طبیعی در صنایع غذایی بسیار گسترش یافته است. هدف پژوهش حاضر بررسی و مقایسه محتوای ترکیبات فنولی و فعالیت آنتی اکسیدانی انگور ارقام رشه و قزل اوزوم می­باشد که توسط کشت بافت در شرایط آزمایشگاهی تولید شدند. برای این منظور جوانه انتهایی ارقام انگور در محیط کشت موراشیگ - اسکوگ کشت داده شد تا گیاهچه کامل ایجاد گردد. سپس عصاره متانولی از برگ­های سالم انگورها تهیه شد و برای انجام آزمون­های تعیین ترکیب پلی فنولی با استفاده از کروماتوگرافی مایع با عملکرد بالا همراه با آشکارساز فرابنفش، گروه­های عاملی با استفاده از طیف سنجی تبدیل فوریه فروسرخ، محتوای فنول کل، فلاونوئید کل، آنتوسیانین کل، کاروتنوئید کل، میزان اسید آسکوربیک، میزان مهار رادیکال­های آزاد 2،2-دی فنیل-1-پیکریلیدرازیل (DPPH) و هیدروکسیل (OH−) استفاده گردید. نتایج نشان داد میزان ترکیبات فنولی کل، فلاونوئید کل، آنتوسیانین کل و کارتنوئید کل عصاره‌های متانولی رقم رشه به ترتیب 10/0±35/50، 05/0±72/29، 20/0±41/10 و 50/0±82/1 میلی گرم بر گرم و در رقم قزل اوزوم به ترتیب 31/0±06/23، 15/0±76/20 ، 15/0±28/7 و 42/0±94/0 میلی گرم بر گرم بود. میزان اسید آسکوربیک، مهار رادیکال­های آزاد DPPH و OH− در عصاره‌های متانولی برگ رشه به ترتیب 04/0± 83/1، 52/1±38/25 و 74/2±04/75 درصد و در برگ قزل اوزوم به ترتیب 01/0±85/0، 05/2±71/18 و 61/3±96/68 درصد بود. به عنوان نتیجه کلی نتایج نشان داد برگ انگور رقم رشه از نظر تمام ویژگی­های بررسی شده بیشتر از برگ انگور رقم قزل اوزوم بود (05/0< p). همچنین این تحقیق نشان داد برگ­های انگور از نظر ترکیبات فنولی و ضد اکسایش­های طبیعی غنی هستند و پتانسیل استفاده در صنایع غذایی و دارویی را دارند.
کلیدواژه‌ها
کشت بافت
متابولیت‌های ثانویه گیاهی
افزودنی طبیعی
انگور

موضوعات

عنوان مقاله English

Comparison of phenolic compounds and antioxidant activity of two grapvine root cultivars Rasha and Qzel Ozum

نویسندگان English

Laya Rezazad Bari 1
Alireza Ghanbari 2
Reza Darvishzadeh 3
Mousa Torabi Giglou 4
Hamed Doulati Baneh 5
1 PhD Student of Physiology and Breeding of Fruit Trees, Department of Horticulture, Faculty of Agriculture and Natural Resources, Mohaghegh Ardebili University, Ardabil, Iran.
2 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Mohaghegh Ardabil University, Ardabil, Iran.
3 Professor, Department of Agricultural Biotechnology, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran.
4 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Mohaghegh Ardebili University, Ardabil, Iran.
5 Associate Professor, Agricultural Jihad Research and Training Center, West Azarbaijan Province, Urmia, Iran.
چکیده English

Nowadays, the use of tissue culture technique in the production of plant secondary metabolites, such as phenolic compounds, as a natural food additive has become very widespread in the food industry. The aim of this study was to investigate and compare the content of phenolic compounds and antioxidant activity of grapevine Rasha and Qzel Ozum cultivars that were produced by tissue culture under in vitro condition. For this purpose, the terminal buds of grape cultivars were cultured in Murashige - Skoog Medium to produce complete seedlings. Then the methanolic extract was prepared from healthy grapevine leaves and to perform polyphenol composition tests using high performance liquid chromatography with ultraviolet detector, functional groups using Fourier-transform infrared spectroscopy, total phenol content, total flavonoids, total anthocyanins, total carotenoids, acid content, ascorbic acid, free radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH), and hydroxyl (OH−). The results showed that the amount of total phenolic compounds, total flavonoids, total anthocyanin and total carotenoids of methanolic extracts of Rasha cultivar were 50.35±0.10, 29.72±0.05, 10.41±0.20, and 1.82±0.50 mg/g, respectively, and in Qzel Ozum cultivar were 23.06±0.31, 20.76±0.15, 7.28±0.15, and 94.94±0.42 mg/g, respectively. Ascorbic acid, DPPH and OH− free radicals scavenging activity in methanolic extracts of Rasha leaves were 1.83±0.04, 25.38±1.52, and 75.04±2.74 %, respectively, and in Qzel Ozum leaves were 0.85±0.01, 18.71±2.05 and 68.96±3.61 %, respectively. As a general result, the results showed that the grape leaves of the Rasha cultivar were more than the grapevine leaves of Qzel Ozum cultivar in terms of all studied characteristics (p <0.05). This study also showed that grape leaves are rich in phenolic compounds and natural antioxidants and have the potential to be used in the food and pharmaceutical industries.

کلیدواژه‌ها English

Tissue culture
Plant secondary metabolites
Natural additive
Grapvine
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