بررسی اثر ضد‌باکتریایی تیمول، کارواکرول، اوژنول و منتول بر چهار باکتری‌ مولد فساد در محصولات کشاورزی و لبنیات

نویسندگان
سپیده عباس‌زاده 1
عقیل شریف‌زاده 2
محبوبه باقری 3
1 مرکز تحقیقات بهداشت و دانشکده بهداشت، پژوهشکده سبک زندگی، دانشگاه علوم پزشکی بقیه‌الله (عج)، تهران، ایران
2 مرکز تحقیقات قارچ‌شناسی، گروه میکروبیولوژی و ایمونولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
3 گروه علوم و صنایع غذایی، دانشکده کشاورزی بردسیر، دانشگاه شهید باهنر کرمان، کرمان، ایران
چکیده
بالا رفتن سطح آگاهی مردم نسبت به اثرات منفی نگهدارنده‌های شیمیایی منجر به انجام تحقیقات بیشتر در زمینه اثر ضدمیکروبی اسانس‌های گیاهی و پتانسیل آنها برای استفاده به عنوان ترکیبات نگهدارنده شده است. در این مطالعه اثر ضدمیکروبی ترکیبات تیمول، کارواکرول، اوژنول و منتول که از مواد موثره‌ی شناخته شده و فراوان در اسانس‌های گیاهی مختلف هستند بر باکتری‌های باسیلوس سرئوس (PTCC 1154)، سودوموناس نیگریفاسینس (ATCC 19375)، اروینیا کاروتوورا (PTCC 1675) و زانتوموناس کامپستریس (PTCC 1473) به روش میکرودایلوشن و انتشار دیسک بررسی شد. در روش میکرودایلوشن، کمترین غلظت بازدارنده رشد 50 ppm گزارش شد که مربوط به اثر ترکیب تیمول بر سودوموناس نیگریفاسینس، اروینیا کاروتوورا و باسیلوس سرئوس بود. در حالیکه بیشترین غلظت بازدارنده رشد برای سودوموناس نیگریفاسینس مربوط به ترکیب منتول (100 ppm) و برای باسیلوس سرئوس، اروینیا کاروتوورا و زانتوموناس کامپستریس مربوط به ترکیب اوژنول (به ترتیب 125، 125 و 108 ppm) تعیین شد. بزرگترین هاله مهار رشد با قطر 8/8 میلی‌متر مربوط به اثر منتول بر سودوموناس نیگریفاسینس بود و کوچکترین هاله مهار رشد با قطر 8/5 میلی‌متر مربوط به اثر اوژنول بر اروینیا کاروتوورا بود. منتول نسبت به سایر ترکیبات اثر ضد میکروبی قویتری نشان داد و اوژنول ضعیف ترین اثر را داشت (p-value<0.05). این در حالیست که هر چهار ترکیب درجات مختلفی از اثر ضد میکروبی بر میکروارگانیسم‌های مولد فساد را داشتند و پتانسیل آنها برای استفاده به عنوان ترکیبات نگهدارنده نشان داده شد.
کلیدواژه‌ها
تیمول
کارواکرول
اوژنول
منتول
ضدمیکروبی

موضوعات

عنوان مقاله English

A study of antimicrobial effect of Thymol, Carvacrol, Eugenol and Menthol on food spoilage bacteria in agricultural crops and dairy products

نویسندگان English

sepideh abbaszadeh 1
aghil sharifzadeh 2
mahboube bagheri 3
1 health research center, life style institute, Baqiyatallah university of medical science, Tehran, Iran
2 mycology research center, department of microbiology and immunology, faculty of veterinary medicine, university of Tehran, Tehran, Iran
3 department of food science and technology, Agricultural faculty of Bardsir, Shahid Bahonar university of Kerman, Kerman, Iran
چکیده English

Currently, consumers are aware of the side-effects of chemical additives and are more interested in natural antibacterial compounds as food preservatives, like herbal essential oils and extracts. In this study, the antimicrobial effect of some main components of the essential oils, including thymol, carvacrol, menthol and eugenol has been evaluated against Bacillus cereus (PTCC 1154), Pseudomonas nigrifacience (ATCC 19375), Erwinia carotorova (PTCC 1675) and Xanthomonas campestris (PTCC 1473) by broth microdilution method and agar disc diffusion method. According to the results, the lowest MIC was reported for Thymol which was 50 ppm (Pseudomonas nigrifacience, Erwinia carotorova and Bacillus cereus) and the maximum MIC for Pseudomonas nigrifacience was 100 ppm due to menthol and 125, 125 and 108 ppm for Bacillus cereus, Erwinia carotorova and Xanthomonas campestris respectively due to Eugenol. Menthol presented the broadest growth inhibitory zone (8.9 mm) for Pseudomonas nigrifacience and the narrowest inhibitory zone was reported for Eugenol against Erwinia carotorova (5.9 mm). Menthol and eugenol were the strongest and weakest antimicrobial agents respectively (p-value<0.05). However, all tested components inhibited the microorganisms at different levels and are potent to be used as food preservatives.

کلیدواژه‌ها English

Thymol
Carvacrol
Eugenol
Menthol
Antimicrobial
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