Ice cream is a unique food product that is very popular in Iran and around the world. As a result of much research into converting this nutrient food into a useful product, the addition of probiotic bacteria, fat reduction, dry matter enhancement and improved health benefits have been done. Probiotics ice cream contains live microbial cells that are well-suited to the distribution of probiotics to consumers. In this study, prebiotic ice cream containing Lactobacillus fermentum strain 4-17 and inulin was prepared at 0, 2.5 and 5% levels. Microbial tests, dry matter measurement, volume increase, pH and acidity determination, melting rate and tissue parameters were evaluated. Evaluation of probiotic bacterial growth over 90 days of survival in different samples showed that the survival rate of this strain in the presence of 5% inulin was about two logarithmic units higher than that produced with 2.5% inulin and about 5 logarithmic units higher than the sample. It was in control. The pH range was 6.8-8.8, 19 to 25 degrees acidity, dry matter was 61.3 to 36.14% and volume increase was 34.38- 15.85%. Also due to the increase in apparent viscosity and decrease in melting rate (from 1.89 to 0.74 g/min), improvement in tissue properties (55% increase in hardness, 69% decrease in adhesion and 29% increase in cohesion) as well as increased product desirability during storage. At 95% confidence level, inulin can be used as an alternative to fat and tissue optimizer.