ارزیابی پتانسیل آنتی‌اکسیدانی، ضدباکتریایی، فنولی و فلاونوئید کل اسانس درمنه سیبری در شرایط آزمایشگاهی

نویسندگان
بهروز علیزاده بهبهانی 1
محمد نوشاد 1
پریسا قاسمی 2
1 1- دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 2- دانشجوی دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.22034/FSCT.22.165.249
چکیده
گیاه درمنه سیبری (Artemisia sieberi) ، یکی از گونه­‌های جنس درمنه (Artemisia) با پراکنش گسترده در ایران و خاورمیانه، به دلیل خواص دارویی متنوع از جمله اثرات ضد­میکروبی مورد توجه قرار گرفته است. هدف از این پژوهش، تعیین محتوای فنول و فلاونوئید کل، فعالیت آنتی­اکسیدانی (مهار رادیکال آزاد DPPH و ABTS) و فعالیت ضد­میکروبی (دیسک و چاهک آگار، حداقل غلظت مهارکنندگی و کشندگی) اسانس درمنه سیبری بود. مقدار فنول و فلاونوئید کل به ترتیب برابر با 53/0±62/44 میلی­گرم گالیک اسید در گرم و 29/0±20/18 میلی­گرم کوئرستین در گرم تعیین شد. فعالیت آنتی­اکسیدانی نیز براساس درصد مهارکنندگی رادیکال آزاد DPPH و ABTS به ترتیب برابر با 57/1±26/58 و 42/1±74/63 به دست آمد. نتایج حاصل از ارزیابی فعالیت ضد­میکروبی به دو روش دیسک و چاهک آگار نشان داد که باکتری­های گرم مثبت استرپتوکوکوس پیوژنز و لیستریا مونوسیتوژنز حساسترین سویه­ها به اسانس درمنه سیبری بودند. حداقل غلظت کشندگی این اسانس روی باکتری­‌های گرم مثبت؛ استرپتوکوکوس پیوژنز، لیستریا مونوسیتوژنز و باسیلوس سرئوس به ترتیب 8 میلی­‌گرم بر میلی­‌لیتر و 128 میلی­‌گرم بر میلی­‌لیتر تعیین شد. همچنین، این اسانس روی باکتری­‌های گرم منفی مانند شیگلا دیسانتری و سالمونلا تیفی موریوم با غلظت 512 میلی­‌گرم بر میلی‌­لیتر موثر بود، به جز کلبسیلا ائروژنز که در غلظت 256 میلی­‌گرم بر میلی‌­لیتر حساسیت نشان داد. بنابراین، از اسانس درمنه سیبری می­‌تواند به عنوان یک ضد­میکرب طبیعی موثر در برابر عفونت­‌های باکتریایی مورد استفاده قرار گیرد.
کلیدواژه‌ها
درمنه سیبری
حداقل غلظت کشندگی
حداقل غلظت مهارکنندگی
رادیکال آزاد DPPH
رادیکال آزادABTS

موضوعات

عنوان مقاله English

Evaluation of the antioxidant, antibacterial, phenolic, and flavonoid potential of the total essential oil of Artemisia sieberi in laboratory conditions

نویسندگان English

Behrooz Alizadeh behbahani 1
Mohammad Noshad 1
Parisa Ghasemi 2
1 1- Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 2- PhD student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

Artemisia sieberi plant (Artemisia sieberi), one of the Artemisia species with wide distribution in Iran and the Middle East, has received attention due to its various medicinal properties, including antimicrobial effects. The aim of this study was to determine the total phenolic and flavonoid content, antioxidant activity (DPPH and ABTS radical scavenging), and antimicrobial activity (agar disk and well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration) of the essential oil of Artemisia sieberi. The total phenolic and flavonoid contents were determined to be 44.62 ± 5.30 mg gallic acid equivalent (GAE)/g and 18.20 ± 2.90 mg quercetin equivalent (QE)/g, respectively. Antioxidant activity, expressed as the percentage of DPPH and ABTS radical scavenging, was found to be 58.26 ± 1.57% and 63.74 ± 1.42%, respectively. The results of the antimicrobial activity evaluation, using both disk diffusion and well diffusion methods, demonstrated that the Gram-positive bacteria Streptococcus pyogenes and Listeria monocytogenes were the most susceptible strains to Artemisia sieberi essential oil. The minimum inhibitory concentration of this essential oil against the Gram-positive bacteria Streptococcus pyogenes, Listeria monocytogenes, and Bacillus cereus was determined to be 8 mg/mL, 128 mg/mL, and 128 mg/mL, respectively. Furthermore, this essential oil was effective against Gram-negative bacteria such as Shigella dysenteriae and Salmonella typhimurium at a concentration of 512 mg/mL, except for Klebsiella pneumoniae, which showed sensitivity at a concentration of 256 mg/mL. Therefore, Siberian wormwood essential oil can be used as an effective natural antimicrobial agent against bacterial infections.

کلیدواژه‌ها English

Artemisia sieberi
Minimum Inhibitory Concentration
Minimum Bactericidal Concentration
DPPH free radical
ABTS free radical
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