تولید و بررسی خواص فیلم زیست تخریب پذیر آنتی اکسیدانی برپایه آرد کدوسبز حاوی نانوذرات کیتوزان بارگذاری شده با اسانس رازیانه

نویسندگان
1 گروه علوم و صنایع غذایی ایران
2 استاد تمام گروه علوم و صنایع غذایی دانشگاه ارومیه
3 دانشیار گروه علوم و صنایع غذایی دانشگاه ارومیه
چکیده
هدف این پژوهش تولید فیلم زیست تخریب پذیر برپایه آرد کدوسبز و بررسی تأثیر افزودن نانوذرات کیتوزان بارگذاری شده با اسانس رازیانه(0، 3، 6 و 9 درصد وزنی/وزنی)بر ویژگی­های فیزیکوشیمیایی و مکانیکی فیلم­ها بود.نتایج نشان داد که افزودن نانوذرات کیتوزان به طور قابل توجهی مقاومت کششی و ازدیاد طول تا نقطه شکست فیلم‌ها را افزایش داد که نشان می‌دهد نانوذرات می‌توانند به عنوان تقویت‌کننده عمل کرده و ساختار فیلم را بهبود بخشند. افزایش درصد نانوذرات باعثافزایش حلالیت در آب و کاهش زاویه تماس آب فیلم‌ها شد. فعالیت آنتی‌اکسیدانی فیلم‌ها با افزایش غلظت نانوذرات به طور قابل توجهی افزایش یافتکه می­تواند به دلیل وجود اسانس رازیانه در نانوذرات و بروز خواص آنتی‌اکسیدانی آن باشد.نتایج آزمون FTIR نشان داد که نانوذرات کیتوزان به خوبی با ماتریس پلیمری فیلم آرد کدوسبز پیوند شیمیایی برقرار کرده‌اند.تصاویر SEM نیز نشان داد که افزودن نانوذرات باعث افزایش زبری سطح فیلم‌ها شده و یکنواختی سطح فیلم با افزایش میزان نانوذرات کیتوزان کمتر شده است.بر اساس نتایج این پژوهش، فیلم حاوی 6 درصد نانوذره کیتوزان حامل اسانس رازیانه، از نظر خواص مکانیکی و فیزیکی بهترین عملکرد را نشان داد. بطور کلی، فیلم خوراکی آرد کدوسبز فعال شده با اسانس رازیانه و نانوذرات کیتوزانمی­تواند به عنوان فیلم خوراکی فعال با خواص مطلوب جهت استفاده در بسته بندی مواد غذایی حساس به فساد اکسایشی معرفی شود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Production and investigation of properties of biodegradable antioxidant film based on zucchini flour containing chitosan nanoparticles loaded with Foeniculum vulgar essential oil

نویسندگان English

Mahsa Abbaspour 1
Mohsen Esmaiili 2
Hadi Almasi 2
Forogh Mohtarami 3
1 Department of Food Science and Technology, Urmia University
2 Professor of Food Science and Technology Department, Urmia University.
3 Associate professor of food science and technology, Urmia University
چکیده English

The objective of this research was to produce biodegradable films based on zucchini flour and to investigate the effect of adding chitosan nanoparticles loaded with fennel essential oil (0, 3, 6, and 9% w/w) on the physicochemical and mechanical properties of the films. The results showed that adding chitosan nanoparticles significantly increased the tensile strength and elongation at the break of the films, indicating that the nanoparticles can act as a reinforcing agent and improve the film structure. Increasing the percentage of nanoparticles increased the water solubility and decreased the water contact angle of the films. The antioxidant activity of the films increased significantly with increasing nanoparticle concentration, which may be due to the presence of fennel essential oil in the nanoparticles and its antioxidant properties. The FTIR test results showed that the chitosan nanoparticles were well chemically bonded to the polymeric matrix of the zucchini flour film. The SEM images also showed that adding nanoparticles increased the surface roughness of the films and the uniformity of the film surface decreased with increasing chitosan nanoparticles. Based on the results of this study, the film containing 6% chitosan nanoparticles loaded with fennel essential oil showed the best mechanical and physical properties. In general, an edible film of zucchini flour activated with fennel essential oil and chitosan nanoparticles can be introduced as an active edible film with desirable properties for packaging food products sensitive to oxidative spoilage.

کلیدواژه‌ها English

Courgette flour
Antioxidant film
Foeniculum vulgar essential oil
Mechanical properties
morphology
Dirpan, A., A.F. Ainani, and M. Djalal, A review on biopolymer-based biodegradable film for food packaging: trends over the last decade and future research. Polymers, 2023. 15(13): p. 2781.
2. Rai, S.K., K. Chaturvedi, and S.K. Yadav, Evaluation of structural integrity and functionality of commercial pectin based edible films incorporated with corn flour, beetroot, orange peel, muesli and rice flour. Food Hydrocolloids, 2019. 91: p. 127-135.
3. Galus, S., et al., Novel materials in the preparation of edible films and coatings—A review. Coatings, 2020. 10(7): p. 674.
4. Emir, A.A., et al., Active films based on Faba bean (Vicia faba L.) flour incorporated with Sumac (Rhus coriaria): Assessment of antioxidant and antimicrobial performances of packaging for shelf life of chicken breast. Food and Bioprocess Technology, 2023. 16(2): p. 327-341.
5. da Silva, A.O., et al., Development and characterization of biopolymer films based on bocaiuva (Acromonia aculeata) flour. International journal of biological macromolecules, 2020. 155: p. 1157-1168.
6. Gutiérrez, T.J., et al., Effect of beet flour on films made from biological macromolecules: Native and modified plantain flour. International Journal of Biological Macromolecules, 2016. 82: p. 395-403.
7. Yildiz, E., et al., Development of pea flour based active films produced through different homogenization methods and their effects on lipid oxidation. Food Hydrocolloids, 2021. 111: p. 106238.
8. Nouraddini, M., M. Esmaiili, and F. Mohtarami, Development and characterization of edible films based on eggplant flour and corn starch. International journal of biological macromolecules, 2018. 120: p. 1639-1645.
9. Kręcisz, M., et al., Physicochemical and quality properties of dried courgette slices: Impact of vacuum impregnation and drying methods. Molecules, 2021. 26(15): p. 4597.
10. Różyło, R., et al., Wheat bread with pumpkin (Cucurbita maxima L.) pulp as a functional food product. Food technology and biotechnology, 2014. 52(4): p. 430-438.
11. Zhang, Y., et al., Effects of zucchini polysaccharide on pasting, rheology, structural properties and in vitro digestibility of potato starch. International Journal of Biological Macromolecules, 2023. 253: p. 127077.
12. Diao, W.-R., et al., Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food control, 2014. 35(1): p. 109-116.
13. Noshad, M. and F. Falah, Investigation of antimicrobial activity of Fennel essential oil on some pathogenic microorganisms causing infection and food poisoning and its interaction with kanamycin antibiotic. Journal of food science and technology (Iran), 2019. 16(91): p. 233-241.
14. Carpena, M., et al., Essential oils and their application on active packaging systems: A review. Resources, 2021. 10(1): p. 7.
15. Zhang, W., et al., Effective strategies of sustained release and retention enhancement of essential oils in active food packaging films/coatings. Food Chemistry, 2022. 367: p. 130671.
16. Mondéjar-López, M., et al., Chitosan nanoparticles loaded with garlic essential oil: A new alternative to tebuconazole as seed dressing agent. Carbohydrate polymers, 2022. 277: p. 118815.
17. Hasheminejad, N., F. Khodaiyan, and M. Safari, Improving the antifungal activity of clove essential oil encapsulated by chitosan nanoparticles. Food chemistry, 2019. 275: p. 113-122.
18. Kaboudi, Z., et al., Nanoencapsulation of Chavir (Ferulago angulata) essential oil in chitosan carrier: Investigating physicochemical, morphological, thermal, antimicrobial and release profile of obtained nanoparticles. International Journal of Biological Macromolecules, 2023. 237: p. 123963.
19. Hadidi, M., et al., Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities. Carbohydrate polymers, 2020. 236: p. 116075.
20. Xu, X., et al., Cinnamon cassia oil chitosan nanoparticles: Physicochemical properties and anti-breast cancer activity. International Journal of Biological Macromolecules, 2023. 224: p. 1065-1078.
21. Song, X., et al., Mandarin (Citrus reticulata L.) essential oil incorporated into chitosan nanoparticles: Characterization, anti-biofilm properties and application in pork preservation. International Journal of Biological Macromolecules, 2021. 185: p. 620-628.
22. International, A., Official methods of analysis of AOAC International. Vol. 17. 2000: AOAC international.
23. Mohtarami, F., Z. Rashidi, and S. Pirsa, Extraction of flaxseed and Plantago Psyllium mucilage: Investigation of rheological properties and efficiency as a fat substitute for the production of low‐calorie cookies. Journal of Food Processing and Preservation, 2022. 46(11): p. e16964.
24. Hosseini, S.F., et al., Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: preparation, characterization and in vitro release study. Carbohydrate polymers, 2013. 95(1): p. 50-56.
25. Yazicioglu, N., Chitosan/teff flour active films incorporated with citric acid and beetroot leaf extract: Physicochemical properties and mathematical modeling of phenolic release. International Journal of Biological Macromolecules, 2024. 270: p. 132301.
26. Shetta, A., J. Kegere, and W. Mamdouh, Comparative study of encapsulated peppermint and green tea essential oils in chitosan nanoparticles: Encapsulation, thermal stability, in-vitro release, antioxidant and antibacterial activities. International Journal of Biological Macromolecules, 2019. 126: p. 731-742.
27. Ebrahimi, S.E., et al., Interactions between Lepidium perfoliatum seed gum–Grass pea (Lathyrus sativus) protein isolate in composite biodegradable film. Food Hydrocolloids, 2016. 54: p. 302-314.
28. Marand, S.A., H. Almasi, and N.A. Marand, Chitosan-based nanocomposite films incorporated with NiO nanoparticles: Physicochemical, photocatalytic and antimicrobial properties. International Journal of Biological Macromolecules, 2021. 190: p. 667-678.
29. Yuan, D., et al., Preparation and characterization of chitosan-based edible active films incorporated with Sargassum pallidum polysaccharides by ultrasound treatment. International Journal of Biological Macromolecules, 2021. 183: p. 473-480.
30. Li, M., et al., Development and characterization of active bilayer film incorporated with dihydromyricetin encapsulated in hydroxypropyl-β-cyclodextrin for food packaging application. Food Hydrocolloids, 2022. 131: p. 107834.
31. Andrade-Mahecha, M.M., D.R. Tapia-Blácido, and F.C. Menegalli, Development and optimization of biodegradable films based on achira flour. Carbohydrate polymers, 2012. 88(2): p. 449-458.
32. Gutiérrez, T.J., In vitro and in vivo digestibility from bionanocomposite edible films based on native pumpkin flour/plum flour. Food Hydrocolloids, 2021. 112: p. 106272.
33. Gutiérrez, T.J., et al., Influence of beet flour on the relationship surface-properties of edible and intelligent films made from native and modified plantain flour. Food Hydrocolloids, 2016. 54: p. 234-244.
34. Li, D. and F. Zhu, Physicochemical, functional and nutritional properties of kiwifruit flour. Food Hydrocolloids, 2019. 92: p. 250-258.
35. Cho, E.J., et al., Nanoparticle characterization: state of the art, challenges, and emerging technologies. Molecular pharmaceutics, 2013. 10(6): p. 2093-2110.
36. Liu, Y., et al., Nanocomplexes film composed of gallic acid loaded ovalbumin/chitosan nanoparticles and pectin with excellent antibacterial activity: Preparation, characterization and application in coating preservation of salmon fillets. International Journal of Biological Macromolecules, 2024. 259: p. 128934.
37. Soleymanfallah, S., et al., Preparation, physical properties, and evaluation of antioxidant capacity of aqueous grape extract loaded in chitosan‐TPP nanoparticles. Food Science & Nutrition, 2022. 10(10): p. 3272-3281.
38. Fan, W., et al., Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique. Colloids and surfaces B: Biointerfaces, 2012. 90: p. 21-27.
39. Cai, M., et al., Antibacterial and antibiofilm activities of chitosan nanoparticles loaded with Ocimum basilicum L. essential oil. International Journal of Biological Macromolecules, 2022. 202: p. 122-129.
40. de Moura, M.R., et al., Highly stable, edible cellulose films incorporating chitosan nanoparticles. Journal of food science, 2011. 76(2): p. N25-N29.
41. Lee, S., et al., Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study. Carbohydrate Polymers, 2021. 262: p. 117930.
42. Ediyilyam, S., et al., Chitosan/gelatin/silver nanoparticles composites films for biodegradable food packaging applications. Polymers, 2021. 13(11): p. 1680.
43. Gasti, T., et al., Chitosan/pullulan based films incorporated with clove essential oil loaded chitosan-ZnO hybrid nanoparticles for active food packaging. Carbohydrate Polymers, 2022. 277: p. 118866.
44. Vahedikia, N., et al., Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes. Colloids and Surfaces B: Biointerfaces, 2019. 177: p. 25-32.
45. Liu, Q., et al., Incorporation of oxidized debranched starch/chitosan nanoparticles for enhanced hydrophobicity of corn starch films. Food Packaging and Shelf Life, 2023. 35: p. 101032.
46. Hosseini, S.F., J. Ghaderi, and M.C. Gómez-Guillén, Tailoring physico-mechanical and antimicrobial/antioxidant properties of biopolymeric films by cinnamaldehyde-loaded chitosan nanoparticles and their application in packaging of fresh rainbow trout fillets. Food Hydrocolloids, 2022. 124: p. 107249.
47. Hosseini, S.F., et al., Fabrication of bio-nanocomposite films based on fish gelatin reinforced with chitosan nanoparticles. Food hydrocolloids, 2015. 44: p. 172-182.
48. Roy, S. and J.-W. Rhim, Fabrication of chitosan-based functional nanocomposite films: Effect of quercetin-loaded chitosan nanoparticles. Food Hydrocolloids, 2021. 121: p. 107065.
49. Yu, X., et al., A hydroxypropyl methylcellulose/hydroxypropyl starch nanocomposite film reinforced with chitosan nanoparticles encapsulating cinnamon essential oil: Preparation and characterization. International Journal of Biological Macromolecules, 2023. 242: p. 124605.
50. Zeng, Y.-F., et al., Preparation and characterization of lotus root starch based bioactive edible film containing quercetin-encapsulated nanoparticle and its effect on grape preservation. Carbohydrate Polymers, 2024. 323: p. 121389.
51. Al-Maqtari, Q.A., et al., Fabrication and characterization of chitosan/gelatin films loaded with microcapsules of Pulicaria jaubertii extract. Food Hydrocolloids, 2022. 129: p. 107624.
52. Venegas, R., et al., Development and characterization of plantain (Musa paradisiaca) flour-based biopolymer films reinforced with plantain fibers. Polymers, 2022. 14(4): p. 748.
53. Sanches, M., et al., Active packaging with starch, red cabbage extract and sweet whey: Characterization and application in meat. LWT–Food Sci. Technol. 2021; 135: 110275. 2020.
54. Ji, S., et al., Preparation, characterization, and evaluation of tamarind seed polysaccharide-carboxymethylcellulose buccal films loaded with soybean peptides-chitosan nanoparticles. Food Hydrocolloids, 2023. 141: p. 108684.
55. Ferreira, R.R., et al., Essential oils loaded-chitosan nanocapsules incorporation in biodegradable starch films: A strategy to improve fruits shelf life. International Journal of Biological Macromolecules, 2021. 188: p. 628-638.
56. Piryaei, M. and S. Azimi, Preparation and evaluation of smart food packaging films with anthocyanin Sardasht black grape based on Astragalus gummifer and chitosan nanoparticles. International Journal of Biological Macromolecules, 2024. 254: p. 127974.
57. Antoniou, J., et al., Characterization of tara gum edible films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study. Food Hydrocolloids, 2015. 44: p. 309-319.
58. Kopczyńska, K., et al., The profile of selected antioxidants in two courgette varieties from organic and conventional production. Antioxidants, 2020. 9(5): p. 404.
59. Thanh, N.C., et al., Antioxidant, anti-inflammatory and anti-proliferative activities of green and yellow zucchini (Courgette). Applied Nanoscience, 2023. 13(3): p. 2251-2260.
60. Kuai, L., et al., Regulation of nano-encapsulated tea polyphenol release from gelatin films with different Bloom values. Food Hydrocolloids, 2020. 108: p. 106045.
61. Liu, T., et al., Development and characterization of novel active chitosan films containing fennel and peppermint essential oils. Coatings, 2020. 10(10): p. 936.
62. Mutlu‐Ingok, A., et al., Antioxidant and antimicrobial activities of fennel, ginger, oregano and thyme essential oils. Food Frontiers, 2021. 2(4): p. 508-518.
63. Sun, Y., et al., Fennel essential oil loaded porous starch-based microencapsulation as an efficient delivery system for the quality improvement of ground pork. International Journal of Biological Macromolecules, 2021. 172: p. 464-474.
64. Soltanzadeh, M., et al., Active gelatin/cress seed gum-based films reinforced with chitosan nanoparticles encapsulating pomegranate peel extract: Preparation and characterization. Food Hydrocolloids, 2022. 129: p. 107620.
65. Fang, M., et al., Fabrication of carboxymethyl chitosan films for cheese packaging containing gliadin-carboxymethyl chitosan nanoparticles co-encapsulating natamycin and theaflavins. International Journal of Biological Macromolecules, 2023. 246: p. 125685.