بررسی خصوصیات ضد میکروبی، آنتی اکسیدانی، فیزیکی و مکانیکی فیلم نانوکامپوزیت (نانوکیتوزان/آلوئه ورا) به همراه پروتئین هیدرولیز شده دانه گوجه فرنگی

نویسندگان
مهسا فلاحتی 1
پیمان آریایی 1
ژاله خوشخو 2
غلامحسن اسدی 3
ابراهیم حسینی 3
1 گروه علوم و صنایع غذایی، واحد آیت ا... آملی، دانشگاه آزاد اسلامی،آمل، ایران
2 گروه علوم و مهندسی صنایع غذایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
3 گروه علوم و مهندسی صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
10.22034/FSCT.21.157.220
چکیده
این تحقیق با هدف بررسی ویژگی­های فیزیکی، مکانیکی، آنتی­اکسیدانی و ضد­میکروبی فیلم هوشمند نانوکیتوزان/آلوئه­ورا حاوی پروتئین هیدرولیز شده دانه گوجه فرنگی انجام شده است. بدین منظور ابتدا پروتئین هیدورلیز شده دانه گوجه فرنگی توسط آنزیم آلکالاز تحت تاثیر زمان­های مختلف (30، 60، 90، 120 دقیقه ) تهیه شد. سپس 5 فیلم خوراکی شامل، نانو کیتوزان، نانوکیتوزان و ژل ­آلوئه­ورا به همراه غلظت­های مختلف پروتئین هیدرولیز شده (0، 5/0، 1 و 5/1%) تهیه و ویژگی‌های فیلم­ها بررسی گردید. بر اساس نتایج مربوط به پروتئین هیدرولیز شده، که پروتئین هیدرولیز شده از میزان پروتئین و درجه هیدرولیز بالایی برخوردار بود. همچنین این پروتئین دارای اسیدهای آمینه هیدروفوب (78/31%) و اسیدهای آمینه آروماتیک (74/11) بالایی بود. نتایج آزمون مکانیکی فیلم نشان داد افزایش غلظت پروتئین سبب کاهش مقاومت کششی، افزایش کشش تا قبل از نقطه پارگی نانو فیلم شد و همچنین بر اساس نتایج مربوط به آزمون­های فیزیکی، افزایش غلظت پروتئین بر روی رطوبت و حلالیت تاثیر معنی­داری نداشت، اما سبب افزایش نفوذ پذیری بخار آب و افزایش کدورت شد (05/0>p). پروتئین هیدرولیز شده دانه گوجه فرنگی توانایی بالایی در فعالیت مهار رادیکال آزاد DPPH و افزایش غلظت تاثیر مثبتی بر این پارامتر داشت (05/0>p)، همچنین این فیلم­ها خاصیت ضد میکروبی بالایی علیه باکتری­های پاتوژن داشتند و خاصیت ضد میکروبی علیه باکتری استافیلوکوکوس اروئوس بالاتر از اشیرشیاکلی بود. فیلم نانوکامپوزیت حاوی 5/1% پروتئین هیدرولیز شده دارای بالاترین فعالیت آنتی­اکسیدانی و ضد­میکروبی را دارا بود (05/0>p). این مطالعه نشان داد که پروتئین هیدرولیز شده می­تواند خواص فیزیکی و مکانیکی فیلم­های بر پایه نانوکیتوزان/آلوئه­ورا را بهبود دهد. به طور خاص، فیلم­های حاوی 5/1% پروتئین هیدرولیز شده دانه گوجه فرنگی دارای خواص بهتری مانند قدرت آنتی­اکسیدانی و خاصیت ضد­میکروبی بودند.
کلیدواژه‌ها
پروتئین گیاهی
فیلم زیست تخریب پذیر
فعالیت ضد‌میکروبی
فعالیت آنتی‌اکسیدانی
باکتری پاتوژن

موضوعات

عنوان مقاله English

Investigation of antimicrobial, antioxidant, physical, and mechanical properties of a nano-composite film (nano-chitosan/ aloe vera) along with hydrolyzed tomato seed protein

نویسندگان English

mahsa falahati 1
peiman ariaii 1
Zhaleh Khoshkhoo 2
Gholamhasan Asadi 3
Ebrahim Hoseini 3
1 Department of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 Department of Food Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran
3 Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده English

This research aimed to investigate the physical, mechanical, antioxidant, and antimicrobial properties of a smart nanocomposite film based on chitosan/ aloe vera containing hydrolyzed tomato seed protein. For this purpose, the hydrolyzed tomato seed protein was first prepared using the Alcalase enzyme under different time conditions (30, 60, 90, 120 minutes). Then, 5 edible films including nano-chitosan, nano-chitosan and aloe vera gel with different concentrations of hydrolyzed protein (0, 0.5, 1 and 1.5%) were prepared, and the film properties were evaluated. Based on the results of the hydrolyzed protein, the hydrolyzed protein had a high protein content and degree of hydrolysis. This protein also had a high content of hydrophobic amino acids (31.78%) and aromatic amino acids (11.74%). The mechanical test results of the films showed that increasing the protein concentration led to a decrease in tensile strength and an increase in elongation at break of the polylactic acid films. According to the physical test results, increasing the protein concentration did not have a significant effect on moisture and solubility, but increased water vapor permeability and turbidity (p<0.05). the hydrolyzed tomato seed protein had high DPPH radical scavenging activities and increasing the concentration had a positive effect on these parameters (p<0.05). These films also had high antimicrobial activity against pathogenic bacteria, with higher antimicrobial activity against Staphylococcus aureus than Escherichia coli. The nanocomposite film containing 1.5% hydrolyzed protein had the highest antioxidant and antimicrobial activity (p<0.05). This study showed that hydrolyzed protein can improve the physical and mechanical properties of chitosan/aloe vera based films. Specifically, films containing 1.5% hydrolyzed protein had better properties such as higher antioxidant activity and antimicrobial activity.

کلیدواژه‌ها English

Plant-based protein
Biodegradable film
Antimicrobial activity
Antioxidant activity
Pathogenic bacteria
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