ارزیابی فعالیت آنتی اکسیدانی، فنول و فلاونوئید کل و فعالیت ضدباکتریایی بابونه آلمانی

نویسندگان
محمد نوشاد
بهروز علیزاده بهبهانی
محمدامین مهرنیا
1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
10.22034/FSCT.21.154.139
چکیده
بابونه آلمانی (Matricaria chamomilla L.) یک گیاه دارویی شناخته شده است که در سراسر جهان توزیع می‌شود و در طب سنتی برای درمان انواع بیماری‌ها کاربرد فراوانی دارد. در این پژوهش، اسانس بابونه آلمانی توسط روش تقطیر با آب استخراج گردید و محتوای فنول کل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی و اثر ضدمیکروبی آن بررسی شد. میزان فنول کل اسانس برابر با mg GAE/g 70/39 و فلاونوئید کل آن برابر با mg QE/g 80/18 بود. فعالیت آنتی‏اکسیدانی اسانس بر پایه دو روش مهار رادیکال‌های آزاد DPPH و ABTS مورد ارزیابی قرار گرفت؛ اسانس قادر به مهار رادیکال‌های آزاد DPPH (60/58 درصد) و ABTS (60/61 درصد) بود. اثر ضد میکروبی اسانس بر علیه باسیلوس سرئوس، استرپتوکوکوس پیوژنز، استافیلوکوکوس اورئوس، شیگلا دیسانتری، انتروباکتر ائروژنز و سالمونلا تیفی­موریوم توسط دیسک دیفیوژن آگار، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی مورد بررسی قرار گرفت. یافته‌های فعالیت ضد میکروبی اسانس به روش دیسک دیفیوژن آگار و چاهک آگار نشان داد که استرپتوکوکوس پیوژنز و انتروباکتر ائروژنز به ترتیب حساس‌ترین و مقاوم‌ترین سویه‌های میکروبی نسبت به اسانس بابونه آلمانی بودند. نتایج این پژوهش نشان داد که اسانس بابونه آلمانی فعالیت آنتی‌اکسیدانی و ضد میکروبی قوی نشان داده است که می‌تواند کاندیدای بالقوه برای تهیه داروهای آنتی‌اکسیدان و ضد میکروب باشد.
کلیدواژه‌ها
بابونه آلمانی
اسانس
ضد میکروب
آنتی‌اکسیدان
ترکیبات فنولی

موضوعات

عنوان مقاله English

Evaluation of antioxidant activity, total phenol and flavonoid and antibacterial activity of German chamomile

نویسندگان English

Mohammad Noshad
Behrooz Alizadeh behbahani
Mohammad Amin Mehrnia
1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

German chamomile (Matricaria chamomilla L.) is a well-known medicinal plant that is distributed worldwide and is widely used in traditional medicine for the treatment of various diseases. In this study, the essential oil of M. chamomilla was extracted by water distillation method and its total phenol content, total flavonoid content, antioxidant activity, and antimicrobial effect were investigated. The total phenol content of the essential oil was 39.70 mg GAE/g and its total flavonoid content was 18.80 mg QE/g. The antioxidant activity of the essential oil was evaluated based on two methods of inhibiting free radicals DPPH and ABTS; the essential oil was able to inhibit free radicals DPPH (58.60 %) and ABTS (61.60 %). The antimicrobial effect of the essential oil against Bacillus cereus, Streptococcus pyogenes, Staphylococcus aureus, Shigella dysenteriae, Enterobacter aerogenes, and Salmonella typhimurium was investigated by disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration. The findings of the antimicrobial activity of the essential oil by the disk diffusion agar and well diffusion agar, showed that S. pyogenes and E. aerogenes were the most sensitive and resistant microbial strains to M. chamomilla essential oil, respectively. The results of this study showed that the essential oil of M. chamomilla has shown strong antioxidant and antimicrobial activity, which can be a potential candidate for the preparation of antioxidant and antimicrobial drugs.

کلیدواژه‌ها English

German chamomile
Essential oil
Antimicrobial
Antioxidant
phenolic compounds
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