ارزیابی فراوانی باکتری های ویبریو کلرا جدا شده از آب و سبزیجات و حضور ژن های حدّت و الگوی مقاومت آنتی بیوتیکی در استان قم

نویسندگان
سمیه کرمانی 1
مجتبی بنیادیان 2
سعید شمس 3
حمدالله مشتاقی 4
1 ، گروه بهداشت و کنترل مواد غذایی ، دانشکده دامپزشکی، دانشگاه شهرکرد ، شهرکرد ، ایران.
2 گروه بهداشت و کنترل مواد غذایی، دانشکده دامپیزشکی، دانشگاه شهر کرد ، شهرکرد ، ایران،
3 مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی قم ، قم ، ایران
4 گروه بهداشت و کنترل مواد غذایی ، دانشکده دامپزشکی، دانشگاه شهرکرد ، شهرکرد ، ایران
10.22034/FSCT.21.154.128
چکیده
ویبریو کلرا یکی از پاتوژن های مهم انسانی است که از طریق آب و غذای آلوده منتقل می شود. بیماری های ناشی از ویبریو کلرا در استان قم به دلایل شرایط آب و هوایی خاص اندمیک است. این مطالعه با هدف بررسی میزان شیوع ویبریو کلرا در آب و سبزیجات استان قم، حضور ژن های حدّت و بررسی الگوی مقاومت آنتی بیوتیکی آن ها انجام شد. در یک دوره دو ساله (0041-1399) ، 120نمونه آب های کشاورزی(70 نمونه) و سبزیجات (50 نمونه) تولید شده در استان قم جمع آوری شدند. نمونه ها روی محیط اختصاصی کشت داده شدند. کلنی های مشکوک رنگ آمیزی گرم شده و آزمون ­های بیوشیمیایی روی آنها انجام شد و با آزمایش سرولوژی، سروتیپ ویبریو کلرا شناسایی شد. سپس بررسی حضور ژن های حدّت با روش PCR و همچنین ارزیابی الگوی مقاومت آنتی بیوتیکی در جدایه ها با روش انتشار در آگار بررسی شد. بطور کلی از 17 نمونه (16/14% (باکتری ویبریو کلرا جدا شد که همه Non-O1 بودند. میزان آلودگی آب و سبزیجات به این باکتری به ترتیب 28/14% (10مورد) و 00/14% (7 مورد ) بود. در ارزیابی ملکولی، میزان فراوانی ژن های حدّت شامل toxR(32 /88%( ، rtxA (82/58%)، hlyA (05/47%)، chxA (88/5%) بود، و100 % جدایه ها فاقد ژن هایctxA،ace و tcpAبودند. بیشترین مقاومت آنتی بیوتیکی مربوط به آمپی سیلین و آموکسی سیلین (29/34 %) و سپس به ترتیب سفوروکسیم (46/17%)، ایمی پنم (76/11%)، سفوکسیتین و تری متوپریم-سولفومتوکسازول (88/5%) بودند. نتایج این مطالعه نشان داد که ویبریو کلرا Non-O1 در آب و سبزیجات استان قم وجود دارد. لذا به عنوان یک منشاء مهم ایجاد بیماری برای انسان، نظارت بهداشتی مستمر بر آب و سبزیجات و ضد عفونی مناسب این مواد غذایی دارای اهمیت زیادی است.
کلیدواژه‌ها
ویبریو کلراNon-O1
ژن های حدّت
مقاومت آنتی بیوتیکی
آب
سبزیجات

موضوعات

عنوان مقاله English

Evaluation of the prevalence of Vibrio cholerae isolated from water and vegetables and the presence of virulence genes and antibiotic resistance pattern in Qom province

نویسندگان English

Somaye Kermani 1
Mojtaba Bonyadian 2
saeed Shams 3
Hamdollah Moshtaghi Boroujeni 4
1 Health and Food Quality Control.Faculty of Veterinary, Shahrekord University, Shahrekord, Iran
2 Department of Health and Food Quality Control, Faculty of Veterinary, Shahrekord University, Shahrekord, Iran.
3 Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.,
4 Department of Health and Food Quality Control, Faculty of Veterinary, Shahrekord University, Shahrekord, Iran
چکیده English

Vibrio cholerae is one of the important human pathogens that is transmitted through contaminated water and food. In Qom province, due to special weather conditions, diseases caused by Vibrio cholerae are endemic. The aim of this study was the prevalence of Vibrio cholerae in water and vegetables of Qom province and the presence of two virulence genes, hlyA and toxR. During two years (2020-2021), 120 samples of agricultural water (70) and vegetables (50) in Qom province were collected. The samples were cultured on specific media. Suspicious colonies were evaluated by Gram staining and biochemical tests and the serotype Vibrio cholerae was identified by serology test. Finally, Then, the presence of virulence genes was investigated by PCR method and also the antibiotic resistance pattern by disk diffusion method was evaluated in the isolates. Vibrio cholera bacteria were isolated from 17 samples (16.14%), all of which were Non-O1. The rate of contamination of water and vegetables was 28.14% (10 cases) and 14.00% (7 cases), respectively. In molecular evaluation, the abundance of virulence genes including: toxR (88.32%), rtxA (58.82%), hlyA (47.05%), chxA (5.88%), and 100% of isolates did not have ctxA, ace and tcpA genes. The most antibiotic resistance is related to ampicillin and amoxicillin (34.29%), followed by cefuroxime (17.46%), imipenem (11.76%), and cefoxitin and trimethoprim-sulfamethoxazole (5.88%). The results of this study showed that Vibrio cholerae Non-O1 is present in water and vegetables of Qom province, and as an important source of disease for humans therefore, continuous health monitoring of water and vegetables and proper disinfection of these foods is very important.

کلیدواژه‌ها English

Vibrio cholerae Non-O1
Virulence genes
Antibiotic resistance
Water
vegetables
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