بررسی ویژگی های شیمیایی و فعالیت ضدباکتریایی اسانس شاهسپران: مطالعه در شرایط برون تنی

نویسندگان
1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
چکیده
این مطالعه با هدف بررسی ترکیبات شیمیایی، اثر آنتی‌اکسیدانی و فعالیت ضدمیکروبی اسانس شاهسپران انجام شد. اسانس شاهسپران با کمک روش تقطیر با آب استخراج شد و سپس محتوای فنول کل (روش فولین سیوکالتو)، فلاونوئید کل (روش رنگ سنجی آلومینیوم کلرید)، اثر آنتی‌اکسیدانی (با استفاده از روش‌های مهار رادیکال آزاد DPPH و ABTS) و ضدمیکروبی (مطابق روش‌های دیسک دیفیوژن آگار، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی) اسانس ارزیابی گردید. محتوای فنول و فلاونوئید کل اسانس به ترتیب برابر با 50/29 میلی‌گرم گالیک اسید و 90/14 میلی‌گرم کوئرستین در گرم بود. فعالیت آنتی‌اکسیدانی اسانس قابل‌توجه بود و به ترتیب قادر به مهار 40/50 درصد و 55/53 درصد رادیکال‌های آزاد DPPH و ABTS بود. مطابق نتایج دیسک دیفیوژن آگار، باسیلوس سوبتیلیس و سالمونلا تیفی به ترتیب با قطر هاله عدم رشد 16 و 50/10 میلی‌متر حساس‌ترین و مقاوم‌ترین سویه‌های باکتریایی نسبت به اسانس بودند. قطر هاله عدم رشد برای این باکتری‌ها در روش چاهک آگار به ترتیب 30/16 و 80/10 میلی‌متر بدست آمد. نتایج حداقل غلظت مهارکنندگی رشد و حداقل غلظت کشندگی برای باکتری باسیلوس سوبتیلیس به ترتیب 4 و 32 میلی‌گرم در میلی‌لیتر و برای باکتری سالمونلا تیفی به ترتیب 64 و بزرگتر از 512 میلی‌گرم در میلی‌لیتر بدست آمد. مطابق نتایج، اسانس شاهسپران را می‌توان بعنوان عامل آنتی‌اکسیدان و ضد میکروب طبیعی در مواد غذایی استفاده نمود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigating the chemical properties and antibacterial activity of Tanacetum balsamita L. essential oil: a study in vitro

نویسندگان English

Behrooz Alizadeh behbahani
Mohammad Noshad
Hassan Barzegar
1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

This study aimed to investigate the chemical composition, antioxidant properties, and antimicrobial activity of Tanacetum balsamita L. essential oil. The essential oil was extracted using the water distillation method, and then the total phenolic content (using the Folin-Ciocalteu method), total flavonoid content (using the aluminium chloride colorimetric method), antioxidant activity (using DPPH and ABTS free radical scavenging methods), and antimicrobial activity (using disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration methods) were evaluated. The total phenolic and flavonoid contents of the essential oil were found to be 29.50 mg gallic acid equivalent per gram and 14.90 mg quercetin equivalent per gram, respectively. The essential oil exhibited significant antioxidant activity, with the ability to scavenge 50.40% and 53.55% of free radicals DPPH and ABTS, respectively. According to the results of disc diffusion agar method, Bacillus subtilis and Salmonella typhi were the most sensitive and resistant bacterial strains, respectively, with zone of inhibition diameters of 16 mm and 10.50 mm. The zone of inhibition diameters for these bacteria in the well diffusion agar method were obtained as 16.30 mm and 10.80 mm, respectively. The minimum inhibitory concentration and minimum bactericidal concentration for B. subtilis were determined as 4 mg/mL and 32 mg/mL, respectively, and for S. typhi, they were 64 mg/mL and greater than 512 mg/mL, respectively. According to the results, T. balsamita essential oil can be used as a natural antioxidant and antimicrobial agent in food products.

کلیدواژه‌ها English

Essential oil
Tanacetum balsamita L
Antimicrobial activity
Antioxidant activity
phenolic compounds
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