ارزیابی پتانسیل آنتی‌اکسیدانی، فنل و فلاونوئید کل و فعالیت ضدمیکروبی اسانس لرگ بر باکتری های بیماری زا: مطالعه در شرایط آزمایشگاهی

نویسندگان
1 1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
2 دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
چکیده
افزودن نگهدارنده های شیمیایی باعث افزایش ماندگاری محصولات غذایی می‌شود، اما استفاده طولانی مدت و بی‌رویه از نگهدارنده های شیمیایی باعث افزایش مقاومت میکروارگانیسم‌ها و خطرات سلامتی مرتبط با جذب آنها می‌شود. گیاهان دارویی هم در دنیا و هم در ایران تنوع زیادی داشته و قابلیت استفاده بعنوان جایگزین ترکیبات شیمیایی را دارا می‌باشند. در این مطالعه، اثر ضد میکروبی اسانس لرگ (Pterocarya fraxinifolia) بر علیه باسیلوس سوبتیلیس، استرپتوکوکوس پیوژنز، استافیلوکوکوس اورئوس، شیگلا دیسانتری، انتروباکتر ائروژنز و سالمونلا تیفی توسط دیسک دیفیوژن آگار، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی بررسی شد. محتوای فنول کل و فلاونوئید اسانس به ترتیب با استفاده از روش‌های فولین سیوکالتو و کلرید آلومینیوم تعیین گردید. برای ارزیابی فعالیت آنتی‏اکسیدانی اسانس از دو روش مهار رادیکال‌های آزاد DPPH و ABTS استفاده شد. محتوای فنول و فلاونوئید کل اسانس 63/38 میلی‌گرم گالیک اسید در گرم و محتوای فلاونوئید آن 20/19 میلی‌گرم کوئرستین در گرم بود. اسانس لرگ قادر به مهار رادیکال‌های آزاد DPPH (60/58 درصد) و ABTS (80/59 درصد) بود. نتایج فعالیت ضد میکروبی اسانس به روش دیسک دیفیوژن آگار و چاهک آگار به خوبی نشان داد که باسیلوس سوبتیلیس و انتروباکتر ائروژنز به ترتیب حساس‌ترین و مقاوم‌ترین سویه‌های میکروبی نسبت به اسانس می‌باشند. حداقل غلظت مهارکنندگی رشد برای این باکتری‌ها به ترتیب 2 و 64 میلی‌گرم در میلی‌لیتر و حداقل غلظت کشندگی به ترتیب 32 و بزرگ‌تر از 512 میلی‌گرم در میلی‌لیتر به دست آمد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Evaluation of antioxidant potential, total phenol and flavonoid and antimicrobial activity of Pterocarya fraxinifolia essential oil on pathogenic bacteria: “in vitro”

نویسندگان English

Hassan Barzegar 1
Behrooz Alizadeh behbahani 2
Mohammad Noshad 1
1 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

Adding chemical preservatives increases the shelf life of food products, but long-term and indiscriminate use of chemical preservatives increases the resistance of microorganisms and health risks associated with their absorption. Medicinal plants have a great diversity both in the world and in Iran and have the potential to be used as alternatives to chemical compounds. In this study, the antimicrobial effect of Pterocarya fraxinifolia essential oil against Bacillus subtilis, Streptococcus pyogenes, Staphylococcus aureus, Shigella dysenteriae, Enterobacter aerogenes, and Salmonella typhi was investigated by disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration. The total phenol and flavonoid content of the essential oil was determined using Folin Ciocalteu and aluminium chloride methods, respectively. The antioxidant activity of the essential oil was evaluated using two methods of inhibiting free radicals DPPH and ABTS. The total phenol and flavonoid content of the essential oil was 38.63 mg of gallic acid/g and the flavonoid content was 19.20 mg of quercetin/g. The essential oil of P. fraxinifolia was able to inhibit free radicals DPPH (58.60%) and ABTS (59.80%). The results of the antimicrobial activity of the essential oil by the disk diffusion agar and well diffusion agar methods showed that B. subtilis and E. aerogenes are the most sensitive and resistant microbial strains to the essential oil, respectively. The minimum inhibitory concentration for these bacteria was 2 and 64 mg/mL, respectively, and the minimum bactericidal concentration was 32 and > 512 mg/mL, respectively.

کلیدواژه‌ها English

Pterocarya fraxinifolia essential oil
natural preservative
Antimicrobial
Antioxidant
Bioactive compounds
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