ارزیابی فنل و فلاونوئید کل، قدرت آنتی‌اکسیدانی و فعالیت ضدمیکروبی اسانس برازمبل بر تعدادی از باکتری‌های گرم مثبت و گرم منفی: یک مطالعه آزمایشگاهی

نویسندگان
1 1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
2 1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده
هدف از این مطالعه تعیین محتوای فنول و فلاونوئید کل و بررسی فعالیت آنتی‌اکسیدانی و ضد میکروبی اسانس برازمبل (Perovskia abrotanoides) بود. محتوای فنول کل با استفاده از روش فولین سیوکالتو، محتوای فلاونوئید کل با کمک روش رنگ سنجی کلرید آلومینیوم، پتانسیل آنتی‌اکسیدانی با استفاده از روش‌های مهار رادیکال آزاد DPPH و ABTS و فعالیت ضد میکروبی با استفاده از روش‌های دیسک دیفیوژن آگار، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت باکتری‌کشی ارزیابی گردید. میزان فنول کل، فلاونوئید کل، مهار رادیکال آزاد DPPH و مهار رادیکال آزاد ABTS اسانس به ترتیب برابر با 26/24 میلی‌گرم گالیک اسید در گرم، 15/13 میلی‌گرم کوئرستین در گرم، 49/52 درصد و 79/56 درصد به دست آمد. نتایج ضدمیکروبی نشان داد که اثر ضدمیکروبی اسانس در برابر باکتری‌های گرم مثبت نسبت به انواع گرم منفی بالاتر بود و استافیلوکوکوس اورئوس و سالمونلا تیفی به ترتیب حساس‌ترین و مقاوم‌ترین سویه‌های میکروبی در برابر اسانس برازمبل بودند. قطر هاله عدم رشد در آزمون دیسک دیفیوژن آگار و چاهک آگار و همچنین حداقل غلظت مهارکنندگی و کشندگی برای باکتری استافیلوکوکوس اورئوس به ترتیب 50/16 میلی‌متر، 30/17 میلی‌متر، 2 میلی‌گرم در میلی‌لیتر و 128 میلی‌گرم در میلی‌لیتر بود و این مقادیر برای باکتری سالمونلا تیفی به ترتیب 60/7 میلی‌متر، 20/9 میلی‌متر، 16 میلی‌گرم در میلی‌لیتر و 512 میلی‌گرم در میلی‌لیتر بود. بطور کلی، اسانس برازمبل می‌تواند بعنوان یک ترکیب آنتی‌اکسیدان و ضد میکروب طبیعی استفاده شود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Evaluation of total phenol and flavonoid, antioxidant power and antimicrobial activity of Perovskia abrotanoides essential oil for study from Gram-positive and Gram-negative laboratories: a laboratory study

نویسندگان English

Mohammad Noshad 1
Behrooz Alizadeh Behbahani 2
Hassan Barzegar 1
1 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

The aim of this study was to determine the total phenol and flavonoid content and investigate the antioxidant and antimicrobial activity of Perovskia abrotanoides essential oil. The total phenol content was determined using the Folin Ciocalteu method, the total flavonoid content using the colorimetric aluminum chloride method, the antioxidant potential using the DPPH and ABTS free radical inhibition methods, and the antimicrobial activity using the disc diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration methods. The total phenol, total flavonoid, DPPH free radical inhibition, and ABTS free radical inhibition of the essential oil were found to be 24.26 mg of gallic acid per gram, 13.15 mg of quercetin per gram, 52.49%, and 56.79%, respectively. The antimicrobial results showed that the antimicrobial effect of the essential oil was higher against Gram-positive bacteria than Gram-negative types, and Staphylococcus aureus and Salmonella typhi were the most sensitive and resistant microbial strains against P. abrotanoides essential oil, respectively. The diameter of the growth inhibition zone in the disc diffusion agar and well diffusion agar tests, as well as the minimum inhibitory and bactericidal concentrations for S. aureus, were 16.50 mm, 17.30 mm, 2 mg/mL, and 128 mg/mL, respectively, and these values for S. typhi were 7.60 mm, 9.20 mm, 16 mg/mL, and 512 mg/mL, respectively. In general, P. abrotanoides essential oil can be used as a natural antioxidant and antimicrobial compound.

کلیدواژه‌ها English

Perovskia abrotanoides
Essential oil
Pathogen
Oxidation
natural preservative
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