خصوصیات ضدمیکروبی، ضد بیوفیلمی و فارماکوکینتیکی برخی از اسانس های خانواده نعناعیان علیه استافیلوکوکوس اورئوس مقاوم به متی سیلین و ونکومایسین

نویسندگان
محمد ولیان 1
ماتیا سادات برهانی 2
اسماعیل باباخانزاده سجیرانی 3
عیسی جرجانی 4
سهراب بوذر پور 4
1 دانشجوی کارشناسی ارشد رشته ژنتیک، دانشکده علوم پایه و فنی مهندسی، دانشگاه گنبدکاووس، گنبدکاووس، ایران
2 استادیار میکروبیولوژی، دانشکده علوم پایه و فنی مهندسی، دانشگاه گنبدکاووس، گنبدکاووس، ایران
3 استادیار گیاهان داروئی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان سمنان (شاهرود)، ایران
4 استادیار ژنتیک، دانشکده علوم پایه و فنی مهندسی، دانشگاه گنبدکاووس، گنبدکاووس، ایران
10.22034/FSCT.20.142.82
چکیده
چکیده:

وجود استافیلوکوکوس اورئوس مقاوم به متی­سیلین (MRSA) و ونکومایسین (VRSA) در مواد غذایی، یک نگرانی جدی برای سلامت عمومی ایجاد کرده است. هدف از این مطالعه، بررسی فعالیت ضدمیکروبی و ضدبیوفیلمی برخی از اسانس‌های خانواده نعناعیان شامل بادرنجبویه، مریم گلی و نعنا فلفلی، بر سویه‌های MRSA و برای اولین بار بر VRSA است. بدین منظور، تست­های دیسک دیفیوژن، تست حداقل غلظت مهاری رشد (MIC)، حداقل غلظت باکتریسیدالی (MBC)، حداقل غلظت مهار تشکیل بیوفیلم (MBIC) و حداقل غلظت موردنیاز برای مهار باکتری‌های موجود در بیوفیلم (MBEC) تعیین شد. سپس ترکیبات مؤثره اسانس­ بادرنجبویه علیه پروتئین­های PBP2a، agrA و Bap با استفاده از اتوداک وینا موردبررسی قرار گرفت و درنهایت، خصوصیات فارماکوکینتیک آن­ها با استفاده از سرورهای ADMETsar و SwissADME بررسی شد. بر اساس نتایج به‌دست‌آمده، میزان MIC و MBC اسانس بادرنجبویه بر روی سویه MRSA به ترتیب برابر با 05/0 و 112/0 میلی‌گرم/میلی‌لیتر و بر روی سویه VRSA به ترتیب برابر با 8/1 و 5/2میلی‌گرم/میلی‌لیتر به دست آمد. میزان MBIC و MBEC اسانس بادرنجبویه در سویه MRSA به ترتیب برابر با 03/0میلی‌گرم/میلی‌لیتر و 112/0میلی‌گرم/میلی‌لیتر و در سویه VRSA به ترتیب برابر با 9/0میلی‌گرم/میلی‌لیتر و 2/3 میلی‌گرم/میلی‌لیتر شد. نتایج داکینگ مولکولی نشان داد که ترکیب بتاکاریوفیلن چه در جایگاه فعال و چه در جایگاه آلوستریک پروتئین PBP2a انرژی اتصالی بالاتری از بقیه ترکیبات موردبررسی نشان می‌دهد (6/6- کیلوکالری بر مول). از طرفی، ترکیبات مؤثره این اسانس، مخصوصاً سیترونلال، تیمول و سیترال ازنظر خصوصیات فارماکوکینتیک قابل‌قبول بودند. از آنجایی که آنتی‌بیوتیک‌های طبیعی می‌توانند جایگزین مناسبی برای آنتی‌بیوتیک‌های معمولی در درمان بیماری‌های ناشی از موادغذایی استافیلوکوکوس اورئوس باشند، نتایج این مطالعه نشان داد که اسانس بادرنجبویه بر رشد و بیوفیلم سویه‌های MRSA و VRSA مؤثر است و می تواند به عنوان یک کاندید داروئی در پیشگیری و درمان عفونت­های ناشی از سویه­های دارای مقاومت آنتی­بیوتیکی این باکتری مورد استفاده قرار گیرد.
کلیدواژه‌ها
استافیلوکوکوس ارئوس
بادرنجبویه
بیوفیلم
داکینگ مولکولی
مقاومت آنتی‌بیوتیکی

موضوعات

عنوان مقاله English

Antibacterial, antibiofilm, and pharmacokinetic properties of some Lamiaceae essential oils against methicillin- and vancomycin-resistant staphylococcus aureus

نویسندگان English

Mohammad Valian 1
Matia Sadat Borhani 2
Esmail Babakhanzadeh Sajirani 3
Eisa Jorjani 4
Sohrab Boozarpour 4
1 MSc student of Genetics, Biology Department, Faculty of Sciences, Gonbad Kavous University, Golestan, Iran.of Genetics, Biology Department, Faculty of Sciences, Gonbad Kavous University, Golestan, Iran.
2 Assistant professor of Microbiology, Biology Department, Faculty of Sciences, Gonbad Kavous University, Golestan, Iran
3 Assistant Professor of Medicinal Plants, Agricultural and Natural Resources Research and Education Center of Semnan Province (Shahrood), AREEO, Shahrood, Iran
4 Assistant professor of Genetics, Biology Department, Faculty of Sciences, Gonbad Kavous University, Golestan, Iran.
چکیده English

The presence of methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) Staphylococcus aureus in food raises a public health concern. This study aimed to investigate the antibacterial and anti-biofilm activity of some Lamiaceae essential oils including Melissa Officinalis, Salvia officinalis, and Mentha piperita against MRSA and for the first time on VRSA strains. For this purpose, the disk diffusion test, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) were determined. Then, M. Officinalis essential oil compounds were investigated against PBP2a, agrA, and Bap proteins using AutoDocK Vina. Finally, pharmacokinetic properties were investigated using ADMETsar and SwissADME servers. Based on the obtained results, the MIC and MBC values of M. Officinalis essential oil against MRSA strain were equal to 0.05 and 0.112 mg/ml, and against VRSA strain were equal to 1.8 and 2.5 mg/ml, respectively. The MBIC and MBEC of M. Officinalis essential oil against MRSA strain were equal to 0.03 mg/ml and 0.112 mg/ml, and against VRSA strain were equal to 0.9 mg/ml and 3.2 mg/ml, respectively. The results of molecular docking showed that β-Caryophyllene had a greater binding affinity to PBP2a protein either in the active site or in the allosteric site (-6.6kcal/mol). On the other hand, the effective compounds of this essential oil, especially citronellol, thymol, and citral, were acceptable in terms of pharmacokinetic properties. Since natural antibiotics can be an alternative to conventional antibiotics in the treatment of Staphylococcus aureus food-borne diseases, the results of this study showed that Melissa Officinalis essential oil is effective on the growth and biofilm of MRSA and VRSA strains, and it can be used as a drug candidate in the prevention and treatment of infections caused by antibiotic-resistant strains of this bacterium.

کلیدواژه‌ها English

Staphylococcus aureus
Melissa Officinalis
Biofilm
molecular docking
Antibiotic resistance
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