اثر دما و زمان نگهداری بر فعالیت زیستی و پایداری فیزیکوشیمیایی نانووزیکول‌های حاوی پروتئین هیدرولیز شده‌ی گرده زنبور عسل

نویسندگان
علیرضا صادقی ماهونک 1
حسین محب الدینی 2
1 استاد، گروه علوم وصنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران
2 دانشیار، گروه علوم دامی، دانشگاه محقق اردبیلی، اردبیل، ایران
10.22034/FSCT.20.142.31
چکیده
در این مطالعه، اثر دما و زمان نگهداری بر فعالیت زیستی و پایداری فیزیکوشیمیایی نانووزیکول­های (لیپوزومها و نیوزومها) حاوی پروتئین هیدرولیز شده­ی گرده زنبور عسل حاصل از آنزیم آلکالاز و پپسین ارزیابی شد. از کیتوزان 2/0 درصد برای پوشش­دهی نانولیپوزوم­ها استفاده گردید. قدرت مهار رادیکالDPPH، قدرت احیا کنندگی یون آهن ، قدرت مهار ACE ، اندازه ذرات، شاخص بس­پاشیدگی، پتانسیل زتا، بازدهی ریزپوشانی و میزان رهایش پروتئین­های هیدرولیز شده از نانووزیکول­ها در مدت 28 روز نگهداری در دمای یخچال و محیط بررسی­شد. نتایج DLS نشان ­داد اندازه نانووزیکول­ها با بارگیری با پروتئین­ هیدرولیز­شده و پوشش­دهی ­با کیتوزان افزایش معنی­دار یافت (P<0.05). نانولیپوزوم­های حاوی­ پوشش­کیتوزان بیشترین­مقدار PDI را داشتند. پتانسیل­زتای نانووزیکول­ها با پوشش­دهی ­با­­ کیتوزان، به بیشترین ­مقدار رسید. نانولیپوزوم­های حاوی پوشش­کیتوزان بیشترین بازدهی ریزپوشانی را داشتند. بعد از 28 روز اندازه نانووزیکول­های با­ پوشش و بدون ­پوشش، 2 تا 26 برابر افزایش­ یافتند. میزان بازدهی­ریزپوشانی نانونیوزوم­ها و نانولیپوزوم­های بدون ­پوشش به­ ترتیب کمترین و بیشترین کاهش را نشان­ دادند. مقادیر فاکتور­های اندازه­گیری شده در طول نگهداری در دمای ­یخچال، به طور معنی­داری کمتر ­از دمای­ محیط بود (P<0.05). با بارگذاری ­پروتئین­های هیدرولیز­شده در نانووزیکول­ها و پوشش­دهی­آنها با­ کیتوزان از کاهش ­فعالیت ­آنتی­اکسیدانی­شان در طول مدت 28 روز نگهداری جلوگیری شد. میزان فعالیت مهارACE در نانولیپوزوم­ها به­طور معنی­داری کمتر از نانونیوزوم­ها بود (P<0.05). بعد از گذشت 28 روز در فعالیت مهار ACE هیدرولیز­شده­های بارگذاری شده در نانولیپوزوم­های بدون پوشش کیتوزان کاهش جزیی مشاهده گردید. این یافته­ها برای طراحی و توسعه غذاهای­کاربردی حاوی پروتئین ­هیدرولیز شده از اهمیت بالایی برخوردار است.
کلیدواژه‌ها
نانووزیکول‌ها
پروتئین هیدرولیز شده
گرده زنبور عسل
فعالیت زیستی
پایداری فیزیکوشیمیایی

موضوعات

عنوان مقاله English

Effect of temperature and storage time on bioactivity and physicochemical stability of nanovesicles containing hydrolyzed bee pollen protein

نویسندگان English

Alireza Sadeghi Mahoonak 1
Hossein Mohebodini 2
1 Professor, Department of Food Sciences and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Iran
2 Associate Professor, Department of Animal Sciences, Mohaghegh Ardabili University, Ardabil, Iran.
چکیده English

In this study, the effect of temperature and storage time on biological activity and physicochemical stability of nanovesicles (liposomes and niosomes) containing bee pollen hydrolyzed protein obtained from alcalase and pepsin enzymatic hydrolysis was evaluated. 0.2% chitosan was used to coat nanoliposomes. DPPH radical scavenging power, ferric ion reducing power, ACE scavenging power, particle size, particle dispersion index, zeta potential, encapsulation efficiency and release rate of hydrolyzed proteins from nanovesicles during 28 days storage at refrigerator and ambient temperature were investigated. Results of DLS showed that the size of nanovesicles increased significantly by loading with hydrolyzed protein and coated with chitosan (P<0.05). Chitosan coated nanoliposomes had the highest amount of PDI. The zeta potential of nanovesicles reached the highest value by coating with chitosan. Chitosan coated nanoliposomes had the highest encapsulation efficiency. After 28 days, the size of coated and uncoated nanovesicles increased 2-26 times.The encapsulation efficiency of nanonisomes and uncoated nanoliposomes showed the lowest and highest decrease, respectively. The values of the measured factors during storage at the refrigerator were significantly lower than ambient temperature (P<0.05). The decline in the antioxidant activities of nanovesicles was significantly prevented by loading hydrolyzed proteins and coating the nanovesicles with chitosan. The ACE inhibition was lower in the nanoliposomes as compared with the nanoniosomes. After 28 days, the ACE inhibition activity of the loaded in nanoliposomes without coating chitosan decreased slightly. These findings are of great importance for designing and developing nutritious foods containing hydrolyzed protein.

کلیدواژه‌ها English

: Nanovesicles
Hydrolyzed protein
Bee pollen
Biological activity
Physicochemical stability
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