بررسی خواص آنتی اکسیدانی پپتیدهای زیست فعال تخلیص شده از ضایعات حاصل از فرآوری ماهی هوور مسقطی(Katsuwonus pelamis)با استفاده از آنزیم های گوارشی پپسین و تریپسین

نویسندگان
شیما کاوه 1
علیرضا صادقی ماهونک 1
وحید عرفانی مقدم 2
محمد قربانی 1
علی اکبر غلامحسین پور 3
مجتبی رئیسی 2
1 گروه علوم و صنایع غذایی، دانشکده صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان
2 مرکز تحقیقات سلامت فرآورده‌های غذایی، دارویی و طبیعی، دانشگاه علوم پزشکی گلستان
3 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه جهرم
10.22034/FSCT.20.141.200
چکیده
در صنایع فرآوری ماهی­ها­ 70-50 % ماهی اولیه به‌عنوان ضایعات تولید می‌شوند درحالیکه منابع غنی از پروتئین و آمینواسیدهای ضروری هستند. استفاده­ی بهینه از این ضایعات و تولید ترکیبات با ارزش افزوده­ی بالا که خواص سلامتی بخش قابل توجهی داشته باشند از چالش‌های مهم صنایع فرآوری ماهی‌ها است. در این پژوهش تاثیر شرایط هیدرولیز (زمان: 300-30 دقیقه و غلظت آنزیم 3-5/0 درصد) و نوع پروتئاز (پپسین و تریپسین) بر درجه هیدرولیز و ویژگی­های آنتی اکسیدانی (مهار رادیکال DPPH، شلاته کنندگی یون آهن، مهار رادیکال نیتریک اکسید، ظرفیت ضداکسایشی کل و احیاء کنندگی یون آهن) پروتئین هیدرولیز شده حاصل از امعاء و احشا ماهی هوور مسقطی با استفاده از روش سطح پاسخ ارزیابی شد. نتایج نشان داد که شرایط بهینه برای تولید پروتئین هیدرولیز شده با بیشترین ویژگی­های آنتی اکسیدانی با آنزیم‌های پپسین و تریپسین به­ترتیب عبارت بودند از: زمان هیدرولیز 09/179 و 62/143 دقیقه و غلظت آنزیم 63/2 و 94/1 درصد؛ در این شرایط درجه هیدرولیز پروتئین­های هیدرولیز شده­ی حاصل از فعالیت تریپسین بیشتر از پپسین محاسبه شد. مقایسه ویژگی­های آنتی اکسیدانی هیدرولیز شده­های حاصل از دوآنزیم مورد استفاده نشان داد که پروتئین هیدرولیز شده حاصل از تریپسین از پتانسیل آنتی اکسیدانی قوی­تری نسبت به پپسین برخوردار بود. بنابراین می­توان بیان نمود که پروتئین هیدرولیز شده امعاء احشا ماهی هوور مسقطی با استفاده از آنزیم تریپسین به­عنوان یک ترگیب سلامتی بخش و با ارزش افزوده بالا قابلیت کاربرد در تولید محصولات غذایی فراسودمند و مکمل­های سلامتی بخش برای ورزشکاران و افراد سالمند را دارد.
کلیدواژه‌ها
آنتی اکسیدان
پپسین
پپتید زیست فعال
تریپسین
هوور مسقطی

موضوعات

عنوان مقاله English

Evaluation the antioxidant properties of purified bioactive peptides from the wastes of skipjack fish (Katsuwonus pelamis) processing, by pepsin and trypsin digestive enzymes

نویسندگان English

shima kaveh 1
Alireza Sadeghi Mahoonak 1
Vahid Erfani Moghadam 2
Mohammad Ghorbani 1
Aliakbar Gholamhosseinpour 3
Mojtaba Raeisi 2
1 Faculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Food, Drug and Natural Products Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
3 Department of Food Science and Technology, Faculty of Agriculture, Jahrom University, Jahrom, Iran.
چکیده English

In fish processing industries, 50-70% of primary fish are produced as waste, while they are rich sources of protein and essential amino acids. The optimal use of these wastes and the production of compounds with high added value that have significant health-giving properties is one of the important challenges of fish processing industries. In this research, the effect of hydrolysis conditions (time: 30-300 minutes and enzyme concentration 0.5-3%) and type of protease (pepsin and trypsin) on the degree of hydrolysis and antioxidant properties (DPPH radical scavenging activity, Fe chelating activity, No radical scavenging activity, total antioxidant capacity and Fe reducing power) of hydrolyzed protein obtained from Skipjack viscera were evaluated using response surface methodology. The results showed that the optimal conditions for the production of hydrolyzed protein with the maximum antioxidant properties with pepsin and trypsin enzymes were respectively: hydrolysis time of 179.09 and 143.62 minutes and enzyme concentration of 2.63 and 1.94 %; In this condition, the degree of hydrolysis of the hydrolyzed proteins resulting from the activity of trypsin was calculated to be higher than that of pepsin. Comparing the antioxidant properties of the hydrolysates obtained from the two enzymes used showed that the hydrolyzed protein obtained from trypsin had a stronger antioxidant potential than pepsin. Therefore, it can be stated that the hydrolyzed protein of the Skipjack viscera using trypsin enzyme as a health-giving supplement and with high added value can be used in the production of functional food products and health supplements for athletes and elderly people.

کلیدواژه‌ها English

Antioxidant
Pepsin
Bioactive peptides
Trypsin
Skipjack
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