غنی‌سازی ماست با روغن درون‌پوشانی‌شده و موسیلاژ دانه چیا: بهینه‌سازی و ارزیابی ویژگی‌های فیزیکی‌وشیمیایی و رئولوژیکی

نویسندگان
1 1دانش‌آموخته کارشناسی ارشد، فناوری مواد غذایی، دانشگاه زنجان
2 2دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده کشاورزی، دانشگاه زنجان، زنجان 38791-45371، ایران
3 3استادیار گروه مواد، انرژی‌ها و فناوری‌های کوآنتومی، دانشکده فناوری‌های همگرا، موسسه رهیافت‌های نوآور برتر پاسارگاد، تهران
چکیده
پژوهش حاضر با هدف بهینه‌سازی فرمولاسیون ماست غنی شده با روغن و موسیلاژ دانه چیا به‌منظور به حداکثر رساندن میزان اسید چرب آلفا-لینولنیک (امگا 3) و حداقل‌ تغییرات در ویژگی‌های فیزیکی‌و‌شیمیایی (pH، اسیدیته قابل‌تیتر، آب‌اندازی، ماده خشک، پراکسید و رنگ) نسبت به نمونه شاهد بود. ابتدا به منظور افزایش پایداری اکسایشی، روغن دانه چیا با استفاده از آلژینات سدیم و موسیلاژ دانه چیا و به کمک روش امولسیون‌‌سازی-ژلاسیون داخلی ریزپوشانی گردید. سپس نمونه‌های ماست حاوی 25/0-0 درصد موسیلاژ دانه چیا و 4-0 درصد روغن دانه چیا به دو صورت آزاد و ریزپوشانی‌‌شده تولید گردید. نمونه فاقد روغن و موسیلاژ دانه چیا به‌عنوان نمونه شاهد می‌باشد. نتایج تصاویر میکروسکوپ الکترونی روبشی نشان‌دهنده تشکیل موفقیت‌آمیز ریزپوشش آلژینات سدیم- موسیلاژ دانه چیا با ساختار پوسته یکنواخت بود. نتایج بدست آمده از ویژگی‌های فیزیکی‌وشیمیایی نشان داد که غنی‌سازی سبب کاهش معنی­دار آب‌اندازی (05/0) و افزایش معنی­دار (05/0) ظرفیت نگهداری و ویسکوزیته ماست گردید. به علاوه ماست حاوی 41/3 درصد روغن دانه چیا به‌صورت ریزپوشانی‌شده و 213/0 درصد موسیلاژ دانه چیا، به عنوان نمونه بهینه تعیین شد. نتایج نشان داد که نمونه بهینه از نظر تعداد باکتری‌های آغازگر نیز تفاوت معنی‌داری با نمونه شاهد ندارد (05/0˃p). بافت ماست­ها، جامد ویسکوالاستیک تشخیص داده شد و در تمام نمونه‌ها همواره در کل محدوده فرکانسی، مدول ذخیره () از مدول افت () بیشتر بود. شاخص رفتار جریان در کلیه نمونه‌هـا کمتر از یک بود که نشان دهنده رفتار غیرنیوتنی (هرشل بالکلی) نمونه‌ها می‌باشد. مدل هرشل بالکی در هردو نمونه بیشترین ضریب تبیین (99/0=R2 ) و کمترین ریشه میانگین مربعات خطا را دارا بود که نشان دهنده مناسب بودن این مدل برای مدل‌سازی رفتار رئولوژیکی نمونه‌ها است.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Fortification of yogurt with encapsulated chia seed oil and mucilage: optimization and evaluation of physicochemical and rheological properties

نویسندگان English

Seyed Zahra Mohamadifard 1
Soheila Zariinghalami 2
Mohsen Zandi 2
Maryam Pakpour 3
1 1Graduated M.s. student, Department of Food Science and Engineering, Faculty of Agriculture, University of Zanjan
2 2 Associate Professor, Department of Food Science and Engineering, Faculty of Agriculture, University of Zanjan, Zanjan 45371-38791, Iran
3 3 Assistant professor, Department of Materials, Energy and Quantum Technologies. Faculty of Convergent Technologies of Sience, Pasargad Institute for Advanced Innovative Solutions (PIAIS), Tehran
چکیده English

The aim of the present study was to optimization of enriched yogurt with chia seed oil and mucilage. This purpose was done with the aim of maximizing omega-3 level and minimizing changes in physical and chemical properties (pH, titrate acidity, syneresis, dry matter, peroxide values and color changes) compared to the control sample. First, in order to increase the oxidative stability, chia seed oil was encapsulated with sodium alginate and chia seed mucilage using emulsification/ internal gelation method. The physical characteristics results showed that the enrichment caused a decrease in the syneresis, an increase in the water holding capacity and viscosity. Then, yogurt was enriched and optimized with free and encapsulated chia seed oil (0 to 4%) and chia seed mucilage (0 to 0.25%). The sample without chia seed mucilage and oil used as a control sample. The results of scanning electron microscopy images showed that the successful alginate-sodium-mucilage microfiber coating of chia seeds has been made with a uniform structure. Yogurt contained 3.41% encapsulated chia seed oil and 0.213% chia seed mucilage was selected as an optimum treatment. The results showed that the number of starter bacteria of optimum sample were not significantly different from the control (p˃0.05). The texture of the yogurts was found to be viscoelastic. The flow behavior index in all samples was less than one, which indicates the non-Newtonian (pseudoplastic) behavior of the samples. Herschel Balky model had R2 and lowest RMSE in both samples, which indicates the suitability of this model to investigate the shear flow behavior in terms of shear velocity of samples.

کلیدواژه‌ها English

Chia seed oil
Chia seed mucilage
Microencapsulation
Enrichment
Yogurt
Optimization
1. Campos, B.E., et al., Optimization of the mucilage extraction process from chia seeds and application in ice cream as a stabilizer and emulsifier. LWT-Food Science and Technology, 2016. 65: p. 874-883.
2. Marcinek, K. and Z. Krejpcio, Chia seeds (Salvia hispanica): health promoting properties and therapeutic applications-a review. Roczniki Państwowego Zakładu Higieny, 2017. 68(2).
3. Kulczyński, B., et al., The chemical composition and nutritional value of chia seeds—Current state of knowledge. Nutrients, 2019. 11(6): p. 1242.
4. Grancieri, M., H.S.D. Martino, and E. Gonzalez de Mejia, Chia seed (Salvia hispanica L.) as a source of proteins and bioactive peptides with health benefits: A review. Comprehensive Reviews in Food Science and Food Safety, 2019. 18(2): p. 480-499.
5. Knez Hrnčič, M., et al., Chia Seeds (Salvia hispanica L.): an overview—phytochemical profile, isolation methods, and application. Molecules, 2020. 25(1): p. 11.
6. Ishak, I., et al., Optimization and characterization of chia seed (Salvia hispanica L.) oil extraction using supercritical carbon dioxide. Journal of CO2 Utilization, 2021. 45: p. 101430.
7. Özcan, M.M., et al., Effect of different microwave power setting on quality of chia seed oil obtained in a cold press. Food Chemistry, 2019. 278: p. 190-196.
8. Kowaleski, J., et al., Functional yogurt with strawberries and chia seeds. Food Bioscience, 2020. 37: p. 100726.
9. Sargi, S.C., et al., Antioxidant capacity and chemical composition in seeds rich in omega-3: chia, flax, and perilla. Food Science and Technology, 2013. 33: p. 541-548.
10. Ullah, R., et al., Nutritional and therapeutic perspectives of Chia (Salvia hispanica L.): a review. Journal of food science and technology, 2016. 53(4): p. 1750-1758.
11. Zandi, M., A. Ganjloo, and M. Bimakr, Encapsulation of musk willow (Salix aegyptiaca L.) essential oil with sodium alginate and whey protein: Characterization, controlled release and mathematical modeling. Journal of food science and technology (Iran), 2022. 19(125): p. 121-133.
12. Zandi, M. and M. Mohebbei, Investigation of encapsulated diacetyl colloidosome release profile as a function of sintering process and release media properties. Flavour and fragrance journal, 2014. 29(6): p. 364-370.
13. Zandi, M., et al., Evaluation of diacetyl encapsulated alginate–whey protein microspheres release kinetics and mechanism at simulated mouth conditions. Food Research International, 2014. 56: p. 211-217.
14. Maes, C., S. Bouquillon, and M.-L. Fauconnier, Encapsulation of essential oils for the development of biosourced pesticides with controlled release: A review. Molecules, 2019. 24(14): p. 2539.
15. Zandi, M., et al., Identification of cardamom encapsulated alginate–whey protein concentrates microcapsule release kinetics and mechanism during storage, stew process and oral consumption. Journal of Food Process Engineering, 2017. 40(1): p. e12314.
16. Zandi, M., Evaluation of the Kinetics of Ascorbic Acid (AA) Release from Alginate‐Whey Protein Concentrates (AL‐WPC) Microspheres at the Simulated Gastro–Intestinal Condition. Journal of Food Process Engineering, 2017. 40(1): p. e12334.
17. Zandi, M., Vitamin protection by Alginate-Whey Protein Micro Gel (AL-WPC MG) as a novel microcapsule against gastrointestinal condition; case study: B-complex vitamins. Iranian Food Science and Technology Research Journal, 2020. 16(3): p. 14.
18. de Campo, C., et al., Nanoencapsulation of chia seed oil with chia mucilage (Salvia hispanica L.) as wall material: Characterization and stability evaluation. Food chemistry, 2017. 234: p. 1-9.
19. Naozuka, J., Elemental enrichment of foods: Essentiality and toxicity. Nutrition & Food Science International Journal, 2018. 4(3): p. 80-84.
20. Mannar, M.V. and N. Khan, Food Fortification: Rationale and Methods. 2016.
21. Ozturkoglu-Budak, S., C. Akal, and A. Yetisemiyen, Effect of dried nut fortification on functional, physicochemical, textural, and microbiological properties of yogurt. Journal of Dairy Science, 2016. 99(11): p. 8511-8523.
22. Us‐Medina, U., et al., Salvia hispanica mucilage‐alginate properties and performance as an encapsulation matrix for chia seed oil. Journal of Food Processing and Preservation, 2017. 41(6): p. e13270.
23. Muñoz-Tébar, N., et al., Enrichment of sheep cheese with chia (Salvia hispanica L.) oil as a source of omega-3. LWT, 2019. 108: p. 407-415.
24. Tamjidi, F., A. Nasirpour, and M. Shahedi, Physicochemical and sensory properties of yogurt enriched with microencapsulated fish oil. Food science and technology international, 2012. 18(4): p. 381-390.
25. Rojas, V.M., et al., Formulation of mayonnaises containing PUFAs by the addition of microencapsulated chia seeds, pumpkin seeds and baru oils. Food chemistry, 2019. 274: p. 220-227.
26. Bakry, A.M., Y.Q. Chen, and L. Liang, Developing a mint yogurt enriched with omega‐3 oil: Physiochemical, microbiological, rheological, and sensorial characteristics. Journal of Food Processing and Preservation, 2019. 43(12): p. e14287.
27. Chan, E.-S., et al., Effects of starch filler on the physical properties of lyophilized calcium–alginate beads and the viability of encapsulated cells. Carbohydrate polymers, 2011. 83(1): p. 225-232.
28. Najafabadi, T. and S. Hoseini Ghaboos, The effect of fat content changes on chemical and rheological properties of yogurt contains Jerusalem artichoke powder during storage. Journal of Food Research, 2020. 29(4): p. 153-169.
29. Najaf Najafi, M., H. Shateri, and M. Kashaninejad, The effect of konjac gum, sage seed gum, homogenization rate and fat content on pH, texture, and color of low fat stirred yogurt. 2021.
30. Ghorbani, M., Yogurt enrichment with Common purslane oil (Portulacaoleracea) and its physicochemical, antioxidant and sensory properties. Journal of food science and technology (Iran), 2019. 16(92): p. 23-36.
31. Kwon, H., et al., Chia seed extract enhances physiochemical and antioxidant properties of yogurt. Journal of dairy science, 2019. 102(6): p. 4870-4876.
32. Karami, P., M. Zandi, and A. Ganjloo, Evaluation of key parameters during ohmic-assisted hydro-distillation of essential oil from aerial part of yarrow (Achillea millefolium L.). Journal of Applied Research on Medicinal and Aromatic Plants, 2022. 31: p. 100425.
33. Jafari, R., M. Zandi, and A. Ganjloo, Effect of ultrasound and microwave pretreatments on extraction of anise (Pimpinella anisum L.) seed essential oil by ohmic-assisted hydrodistillation. Journal of Applied Research on Medicinal and Aromatic Plants, 2022. 31: p. 100418.
34. Izadi, Z., et al., Optimization of producing enriched yogurt with phytosterols in order to reducing cholesterol content. Iranian Food Science & Technology Research Journal, 2011. 7(2): p. 156-164.
35. Atik, D.S., et al., Chia seed mucilage versus guar gum: Effects on microstructural, textural, and antioxidative properties of set-type yoghurts. Brazilian Archives of Biology and Technology, 2020. 63.
36. Ghorbanzade, T., et al., Nano-encapsulation of fish oil in nano-liposomes and its application in fortification of yogurt. Food chemistry, 2017. 216: p. 146-152.
37. Ashrafi, R. and N. Gheybi, Investigation the effect of Dill extract (Anethume graveolens) using on the antioxidant and physicochemial properties of set yogurt. JFST, 2019. 84(15): p. 203-215.
38. Nourmohammadi, N., S. Soleimanian‐Zad, and H. Shekarchizadeh, Effect of Spirulina (Arthrospira platensis) microencapsulated in alginate and whey protein concentrate addition on physicochemical and organoleptic properties of functional stirred yogurt. Journal of the Science of Food and Agriculture, 2020. 100(14): p. 5260-5268.
39. Gheybi, N. and R. Ashrafi, The Effect of Inulin and Quince Seed Gum Powder on the Physicochemical and Qualitative Properties of Low Fat Yogurt. Iranian Journal of Biosystems Engineering, 2020. 50(4): p. 963-975.
40. Marand, M.A., et al., Fortification of yogurt with flaxseed powder and evaluation of its fatty acid profile, physicochemical, antioxidant, and sensory properties. Powder Technology, 2020. 359: p. 76-84.
41. Kim, S.Y., et al., The quality characteristics, antioxidant activity, and sensory evaluation of reduced-fat yogurt and nonfat yogurt supplemented with basil seed gum as a fat substitute. Journal of dairy science, 2020. 103(2): p. 1324-1336.
42. Shahdadi, F., Study of various concentrations of resistant starch and chitosan on microstructure, rheological properties and viability of encapsulated probiotic bacteria in drinking yoghurt. Journal of Food Processing and Preservation, 2017. 9(2): p. 69-84.
43. Ein Ali Afjeh, M., et al., Effect of lactose, whey protein concentrat and skim milk powder on some rheological and sensory properties of set yoghurt with different somatic cell count. Journal of food science and technology (Iran), 2015. 12(49): p. 209-218.