بررسی زنده مانی لاکتوباسیلوس اسیدوفیلوس ریزپوشانی شده در امولسیون دوگانه با صمغ فارسی در طول ذخیره سازی و شرایط شبیه سازدستگاه گوارش

نویسندگان
خزر پورعلی رشتی نژاد 1
محمد محسن زاده 2
محمد مالکی 3
1 کارشناس ارشد بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، ایران
2 استاد گروه بهداشت مواد غذایی و آبزیان، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، ایران.
3 پژوهشگر پسا دکتری تخصصی بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، ایران
10.22034/FSCT.19.134.61
چکیده
ریز پوشانی روشی رایج برای بهبود قابلیت زنده مانی باکتری‌های پروبیوتیک در برابر تنش‌های محیطی است. در این پژوهش با استفاده از امولسیون دوگانه با صمغ فارسی ، پایداری امولسیون، خصوصیات فیزیکوشیمیایی و مورفولوژیکی، راندمان ریز پوشانی و زنده مانی ریز پوشینه‌ها، در طول ذخیره‌سازی و محیط شبیه‌ساز دستگاه گوارش موردبررسی قرار گرفت. اندازه ذرات، پتانسیل زتا و شاخص پراکندگی به ترتیب معادل nm525، mv68/44- و 33/0 بود. راندمان ریز پوشانی 88 % و پایداری امولسیون بین 85 % تا 43/95 % بود. ریزپوشانی لاکتوباسیلوس اسیدوفیلوس در امولسیون‌ دوگانه (W/O/W) با صمغ فارسی باعث بهبود زنده مانی آن در شرایط نگهداری در دمای °C 4 به مدت 28 روز شد. جمعیت باکتریایی در تیمارهای ریز پوشانی نشده بعد از گذشت 7 روز به صفر رسید درحالیکه در تیمارهای ریز پوشانی شده فقط log 58/0 کاهش داشت. در محیط شبیه‌ساز معده تعداد باکتری‌ها برای تیمار ریز پوشانی شده log 97/1 و برای تیمار حاوی باکتری آزاد log 9/3 کاهش یافت، و در محیط شبیه‌ساز روده نیز برای تیمار ریز پوشانی شده log 45/0 و برای تیمار حاوی باکتری آزاد log 3/1 کاهش یافت. نتایج نشان داد که استفاده از امولسیون دوگانه صمغ فارسی باعث بهبود زنده‌مانی لاکتوباسیلوس اسیدوفیلوس در شرایط نگهداری در دمای °C 4 و محیط شبیه‌سازی‌شده دستگاه گوارش می‌شود.
کلیدواژه‌ها
پروبیوتیک
ریز پوشانی
زنده‌مانی
لاکتوباسیلوس اسیدوفیلوس
امولسیون

موضوعات

عنوان مقاله English

Investigating the survival of microencapsulated Lactobacillus acidophilus in double emulsion with Persian gum during storage and digestive simulating conditions

نویسندگان English

Khazar Pourali Rashti-Nejad 1
Mohammad Mohsenzadeh 2
Mohammad Maleki 3
1 Department of Food Hygiene and Aquaculture, Factulty of Veterinary Medicine, Ferdowsi University of Mashhad (FUM), Mashhad, Iran
2 Department of Food Hygiene and Aquaculture, Factulty of Veterinary Medicine, Ferdowsi University of Mashhad (FUM), Mashhad, Iran
3 Department of Food Hygiene and Aquaculture, Factulty of Veterinary Medicine, Ferdowsi University of Mashhad (FUM), Mashhad, Iran
چکیده English

Microencapsulation is a common method to improve the viability of probiotic bacteria against environmental stresses. In this research, by using double emulsion with Persian gum, emulsion stability, physicochemical properties, microcoating efficiency and microcoating viability were investigated during storage and in a simulated gastrointestinal conditions of the digestive system. Encapsulation of Lactobacillus acidophilus in double emulsion (W/O/W) with Persian gum improved the survival under storage conditions at 4 °C for 28 days, and only 0.58 log decreased and free bacteria after passage 7 days reached zero. The microcoating efficiency was 88% and the emulsion stability was between 85% and 95.43%. The optical microscope image showed a distinct double emulsion. In the simulating conditions of the stomach, the number of bacteria decreased by 1.97 log for the micro-coated treatment and by 3.9 log for the treatment containing free bacteria, and in the simulating conditions of the intestine, it decreased by 0.45 log for the micro-coated treatment and for the treatment containing free bacteria. 1.3 decreased. The particle size was (525 nm), zeta potential (-44.68 mv) and dispersion index (0.33). The results showed that the use of Persian gum double emulsion improves the survival of Lactobacillus acidophilus against storage conditions and the simulated environment of the digestive system.

کلیدواژه‌ها English

Probiotic
Microencapsulation
Survival
Lactobacillus acidophilus
Emulsion
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