بررسی خواص آنتی‌اکسیدانی و عملکردی کلاژن و کلاژن هیدرولیز شده از پوست ماهی هامور (Epinephelus coioides)

نویسندگان
رضوان موسوی ندوشن 1
فاطمه عسگری مهرابادی 2
1 دانشیار گروه علوم و صنایع غذایی ، واحد تهران شمال، دانشگاه آزاد اسلامی ، تهران، ایران
2 کارشناس ارشد گروه علوم و صنایع غذایی ، واحد تهران شمال، دانشگاه آزاد اسلامی ، تهران، ایران.
10.22034/FSCT.19.126.387
چکیده
کلاژن فراوان‌ترین پروتئین با منشأ حیوانی و مهم‌ترین ترکیب ساختمانی بافت پیوندی است که در صنعت غذا و نوشیدنی‌ مورد استفاده قرار می‌گیرد. علاوه بر این تحقیقات اخیر ویژگیهای عملکردی، فراسودمند و پتانسیل مصرف کلاژن استخراج شده از ضایعات آبزیان را در قالب غذا-دارو و سامانه های رسانش ترکیبات حساس زیست فعال به اثبات رسانده است. لذا هدف از این تحقیق بررسی ویژگی‌های ساختاری، خواص آنتی‌اکسیدانی و عملکردی در کلاژن و کلاژن هیدرولیز شده از پوست ماهی هامور معمولی(Epinephelus coicoides) قرار داده شد. دراین تحقیق کلاژن به روش اسیدی و آنزیمی استخراج و سپس کلاژن های استخراج شده توسط آنزیم پپسین هیدرولیز گردید. ویژگیهای ساختاری آنها به‌وسیله‌ی (FTIR)، (SEM) و ویژگیهای عملکردی شامل DPPH , قدرت کاهندگی ، میزان تولید ، خصوصیات و پایداری کف حاصل از کلاژن و کلاژن هیدرولیز شده مورد ارزیابی قرار گرفت. تصاویر میکروسکوپ الکترونی نشانگر ساختار حفاظت شده ورقه ای ؛ فیبریلی و ساختار فضایی دوم و سوم بوده، شبکه ماتریکس از منافذ متحدالشکل تشکیل شده، در طیف (FTIR) حضور گروه های عاملی شاخص در کلاژن نوع I مشتمل بر پیوندهای آمید A و B همچنین امیدهای نوع II,IوIII به اثبات رسید. نتایج به‌دست‌آمده در مورد خواص آنتی اکسیدانی نشان داد با افزایش غلظت، و بویژه در کلاژن هیدرولیز شده قدرت کاهندگی و توانایی مهار رادیکال DPPH افزایش پیداکرده، همچنین بررسی کلاژن و نمونه هیدرولیز شده نشان داد که تنها کلاژن پوست ماهی هامور پتانسیل تولید کف دارد. در نهایت می توان گفت کلاژن و کلاژن هیدرولیز و استخراج شده از پوست ماهی هامور به عنوان یک ماهی پرمصرف دریایی با خواص آنتی اکسیدانی مطلوب، و ویژگیهای عملکردی مناسب میتواند در مقیاس انبوه تولید و به عنوان یک ترکیب زیست فعال و امولسیفایر در صنعت غذا و در تولید محصولات فراسودمند مورد استفاده قرار گیرد.
کلیدواژه‌ها
کلاژن
پوست
ماهی هامور معمولی(Epinephelus coicoides)
خواص انتی اکسیدانی
خواص عملکردی
کلاژن هیدرولیزشده

موضوعات

عنوان مقاله English

antioxidant and functional properties of collagen from fish skin hamur (Epinephelus. Coioides)

نویسندگان English

Rezvan Mousavi nadushan 1
Fatemeh asgari mehrabadi 2
1 Associate Professor, Department of food science and technology, North Tehran Branch, Islamic Azad University,Tehran, Iran.
2 MSc., Department of food science and technology, North Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده English

Collagen is the most abundant protein of animal origin and the most important compound in the connective tissue which is widely used in the food and beverage industry. In addition, recent research has demonstrated the functional, beneficial, and potential properties of collagen extracted from aquatic wastes as nutraceuticals and for delivery systems of sensitive bioactive compounds. Therefore, the aim of this research was considered to study the structural charachters, antioxidant and functional properties of collagen and collagen hydrolysate extracted from the skin of common grouper (Epinephelus coicoides). In the present experimental study, the collagen sample was extracted by acid and enzymatic method and structural characters were studied by Fourier-transform infrared (FTIR) spectroscopy, Scanning Electron Microscope (SEM) and polyacrylamide gel electrophoresis (SDS-PAGE). DPPH radical scavenging, reducing Power and foam producing capacity also assessed.The results of Scanning Electron Microscope confirmed schistose structure, filamentus network, secondary and third structure, and the good interconnection and uniform porus matrix was noticed. The typical characters for type I collagen, including five peaks (amide A, amide B, amide I, amide II and amide III are observed in the spectrogram of collagen and collagen hydrolysate, also the preserved triple-helical structure were comfirmed.


The results of the functional properties and antioxidant activity of collagen from orange-spotted grouper skin also showed that with increasing the concentration, reducing power and DPPH radical scavenging ability increased. Comparing collagen and hydrolyzed sample, the foam producing capacity was proved just for collagen. Finally, it can be said that collagen and hydrolyzed collagen extracted from the skin of orange-spotted grouper with suitable antioxidant properties, and appropriate functional properties can be mass-produced as a bioactive compound and as emulsifier may be used in the food industry and in the production of Functional foods.

کلیدواژه‌ها English

Collagen
skin
Epinephelus coicoides
Antioxidant properties
Functional properties
Collagen hydrolysate
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