بررسی اثر مصرف رژیم غذایی پر چرب و لاکتوباسیلوس روتری بر وزن بدن، فلور روده و شاخص‌های متابولیکی موش C57BL/6

نویسندگان
احسان شاد 1
سید شهرام شکرفروش 2
سعید نظیفی 3
محمدهادی اسکندری 1
1 بخش علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران
2 بخش بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
3 بخش مطالعات بالینی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
10.22034/FSCT.19.126.269
چکیده
دراین مطالعه اثر رژیم غذایی و پروبیوتیک بر خصوصیات بدن موش­های C57BL /6 بررسی گردید. موش­ها به چهار گروه شامل گروه کنترل، گروه دریافت کننده پروبیوتیک لاکتوباسیلوس روتری DMC20016 ، گروه دریافت کننده جیره غذایی پرچرب (HFD) و گروه دریافت کننده پروبیوتیک و جیره پرچرب تقسیم شدند. تأثیر رژیم غذایی پرچرب و پروبیوتیک بر فلورمیکروبی روده، وزن بدن، فاکتورهای خونی، هورمون لپتین و لیپوپلی ساکارید پس از هشت هفته نگهداری در آن­ها مورد بررسی قرار گرفت. رژیم غذایی پرچرب باعث افزایش وزن توده بدن، چربی شکمی و کبد شد. گروه HFD بیشترین افزایش وزن بدن (g 02/1 ± 36/8) نسبت به سایرگروه­ها داشت و مصرف لاکتوباسیلوس روتری باعث کاهش وزن بصورت معنی­دار نگردید. همچنین رژیم غذایی پرچرب به طور قابل توجهی لیپوپلی ساکارید و لپتین را افزایش داد، اما لاکتوباسیلوس روتری باعث کاهش این شاخص­ها در مقایسه با گروه HFD شد. فراوانی و تنوع فلور میکروبی روده به رژیم غذایی و پروبیوتیک مصرفی بستگی داشت. با مصرف رژیم غذایی پرچرب میزان فرمیکوت (70%) افزایش و باکتریوئیدیت­ها (1%>) کاهش یافت. اما با مصرف لاکتوباسیلو روتری میزان اکتینوباکتری­ها (4%) و فرمیکوت­ها (16%) نسبت به نمونه کنترل کاهش و میزان پروتئوباکتری­ها (78%) افزایش یافت. با توجه به نتایج این مطالعه و تحقیقات مشابه، همه پروبیوتیک­ها بر شاخص­های چاقی موثر نیستند و باید براساس هدف مصرف، مکمل پروبیوتیک انتخاب گردد.
کلیدواژه‌ها
پروبیوتیک
تغییر وزن
چاقی
لاکتوباسیلوس روتری
فلور میکروبی

موضوعات

عنوان مقاله English

The Effects of High-fat Diet and lactobacillus reuteri on Body Weight, Metabolic profiles and Gut Microbiota of C57BL/6 Mice

نویسندگان English

Ehsan Shad 1
Seyed shahram Shekarforoush 2
nazifi s nazifi 3
Mohammad hadi Eskandari 1
1 Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, Iran
2 Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3 Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
چکیده English

This study investigated the effects of diet and probiotic on the body characteristics of C57BL/6 mice. Mice were divided into four groups: the control group, Lactobacillus reutrei DMC20016 probiotic group, high-fat diet (HFD) group, and probiotic with the high-fat diet group. After eight weeks of storage, a high-fat diet and probiotic effects on gut microbiota, body weight, blood factors, leptin hormone, and lipopolysaccharide were studied. A high-fat diet has increased body weight, fat mass, and liver weight. The HFD group had the highest body weight gain (8.36 ± 1.02 gr) compared to the other groups, and consumption of Lactobacillus reuteri did not show a significant effect on body weight. The high-fat diet also significantly increased lipopolysaccharide and leptin, but Lactobacillus reuteri decreased these parameters compared to the HFD group. The abundance and diversity of gut microbiota depended on diet and probiotics consumed. With the consumption of a high-fat diet, the number of Firmicutes ( 70%) increased and Bacteroidetes (<1%) decreased. However, the amount of Actinobacteria (4%) and Firmicutes (16%) decreased, and the amount of Proteobacteria (78%) increased in the H.LR group compared to the control sample. According to this study and similar research, not all probiotics are effective on obesity indicators, and probiotic supplements should be selected based on the purpose of use.

کلیدواژه‌ها English

Gut Microbiota
Lactobacillus reuteri
Obesity
Probiotic
Weight Change
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