ارزیابی روش های مختلف استخراج ترکیبات فیتوشیمیایی و آنتی اکسیدانی عصاره برگ مورینگا الیفرا

نویسندگان
سعید دغاغله 1
علیرضا کیاست 2
سید محمد صفی الدین اردبیلی 3
رویا میرزاجانی 4
1 دانشجوی کارشناسی ارشد، دانشکده علوم پایه، گروه شیمی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 استاد، دانشکده علوم پایه، گروه شیمی، دانشگاه شهید چمران اهواز، اهواز، ایران
3 استادیار، دانشکده کشاورزی، گروه مهندسی بیوسیستم، دانشگاه شهید چمران اهواز، اهواز، ایران
4 دانشیار، دانشکده علوم پایه، گروه شیمی، دانشگاه شهید چمران اهواز، اهواز، ایران
10.52547/fsct.18.121.13
چکیده
مورینگا الیفرا (Moringa oleifera) به عنوان یک منبع غنی و مقرون به‌صرفه از ترکیبات فیتوشیمیایی شناخته شده می‌باشد و دارای کاربردهای فراوانی در صنعت، تصفیه‌آب، داروسازی و تولید سوخت‌های زیستی است، برگ گیاه مورینگا الیفرا توسط مرکز گیاهی تایوان از میان 120 نوع گونه مواد غذایی مورد مطالعه به عنوان گیاهی دارای بالاترین محتوای تغذیه‌ای معرفی شد. در این پژوهش تاثیر روش‌های مختلف استخراج از قبیل ماسراسیون و سوکسله برمیزان محتوای فنولی، فلاونوئیدی و خواص آنتی‌اکسیدانی، همچنین اثر این عصاره‌ها بر روی سلول‌های سرطانی A549 ریه به وسیله آزمون MTT بررسی گردید. طبق نتایج بدست آمده از برگ گیاه مورینگا الیفرا بیشترین بازده استخراج مربوط به عصاره‌گیری به روش سوکسله بود. بیش‌ترین محتوای فنولی و فلاونوئیدی مربوط به روش استخراج ماسراسیون بود. نتایج حاصل از بررسی خواص آنتی‌اکسیدانی عصاره نشان داد که بیشترین خواص آنتی‌اکسیدانی مربوط به عصاره حاصل از روش سوکسله بود. آزمون MTT نشان داد که از بین عصاره‌های مختلف کم‌ترین IC50 مربوط به عصاره استخراجی توسط روش سوکسله می‌باشد. این مطالعه نشان می دهد که برگ های مورینگا الیفرا یک منبع تغذیه ای مناسب است.
کلیدواژه‌ها
مورینگا الیفرا
استخراج
محتوای فنولی
خواص آنتی‌اکسیدانی

موضوعات

عنوان مقاله English

Evaluation of different extraction methods of phytochemical and antioxidant compounds of Moringa oleifera leaf extract

نویسندگان English

Saied Daghaghele 1
Ali Reza Kiasat 2
saeid mohammad safiodin ardebili 3
Roya Mirzajani 4
1 MSc Student in Phytochemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Professor, Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 Assistant Professor, Department of Biosystems Engineering, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
4 Associate Professor, Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده English

Moringa oleifera is known as a rich and cost-effective source of phytochemical compounds and has many applications in industry, water treatment, pharmacy and biofuels The leaves of Moringa oleifera were introduced by the Taiwan Plant Center as one of the 120 food species studied as the plant with the highest nutritional content. In this study, the effect of different extraction methods such as Maceration and Soxhlet on phenolic content, flavonoid content and antioxidant properties, The effect of these extracts on A549 lung cancer cells was also evaluated by MTT assay. This plant was studied. According to the results obtained from the leaves of Moringa oleifera, the highest extraction efficiency was related to Soxhlet extraction. The highest phenolic and flavonoid content was related to Maceration extraction method. The results of studying the antioxidant properties of the extract showed that most of the antioxidant properties were related to the extract obtained by Soxhlet method. MTT test showed that among the various extracts, the lowest IC50 was related to the extract extracted by Soxhlet method. This study shows that Moringa oleifera leaves are a good nutritional source.

کلیدواژه‌ها English

Moringa oleifera
cancer cells
Extract
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