طراحی بسته بندی نانوکامپوزیت برپایه بیوپلیمر ژلاتین حاوی نانوذره دی اکسید تیتانیوم و عصاره زعفران به منظور کاربرد در بسته بندی مواد غذایی

نویسندگان
شمیمه عظیمی سلیم 1
مریم عزیزی لعل آبادی 2
میلاد توسلی 3
محمود علیزاده ثانی 4
1 کمیته تحقیقات دانشجویی ، دانشگاه علوم پزشکی کرمانشاه ، کرمانشاه ، ایران
2 مرکز تحقیقات عوامل موثر بر محیط زیست در سلامت (RCEDH) ، انستیتو سلامت، دانشگاه علوم پزشکی کرمانشاه ، کرمانشاه ، ایران
3 کمیته تحقیقات دانشجویی ، گروه علوم و صنایع غذایی ، دانشکده تغذیه و علوم غذایی ، دانشگاه علوم پزشکی تبریز ، تبریز ، ایران
4 دانشجوی دکتری تخصصی، رشته بهداشت و ایمنی مواد غذایی، گروه بهداشت و ایمنی مواد غذایی، دانشکده بهداشت، دانشگاه علوم پزشکی تهران.
10.52547/fsct.18.121.3
چکیده
در این مطالعه بسته بندی زیست تخریب پذیر برپایه بیوپلیمر ژلاتین (در غلظت 3 درصد وزنی-وزنی) حاوی نانو ذره دی اکسید تیتانیوم (در غلظت 1 درصد وزنی-وزنی) و عصاره زعفران (در غلظت 2 درصد حجمی-حجمی) با روش تبخیر-حلال سنتز شد. در این پژوهش خصوصیات فیزیکی (ضخامت، شفافیت، محتواب رطوبتی، میزان حلالیت و نفوذپذیری به بخار آب)، مکانیکی، خصوصیات ضد میکروبی و آنتی اکسیدانی، خصوصیات ساختاری و ظاهری فیلم ها مورد بررسی قرار گرفت. پس از آنالیز داده ها، نتایج نشان داد که تأثیر عصاره زعفران و نانو ذرات دی اکسید تیتانیوم بر تمام خصوصیات مورد بررسی معنی‌دار (P<0.05) بود. افزودن نانو ذرات دی اکسید تیتانیوم و عصاره زعفران باعث افزایش ضخامت، و بهبود خصوصیات مکانیکی و کاهش محتوای رطوبتی، نفوذپذیری به بخار، شفافیت، و حلالیت شد. همچنین فیلم های نانوکامپوزیت حاوی نانوذرات دی اکسید تیتانیوم و عصاره زعفران خصوصیات آنتی اکسیدانی (80%~) و اثرات ضد میکروبی قابل قبولی به ویژه بر علیه باکتری گرم مثبت استافیلوکوکوس اورئوس را نشان دادند. با توجه به نتایج بدست آمده از این مطالعه، این نوع بسته بندی می تواند به عنوان جایگزین مناسب برای بسته بندی های سنتزی پیشنهاد گردد.
کلیدواژه‌ها
فیلم تجزیه پذیر
عصاره زعفران
بسته بندی نانوکامپوزیت
نانوذرات دی اکسید تیتانیوم
خواص آنتی اکسیدانی

موضوعات

عنوان مقاله English

Design of nanocomposite packaging based on gelatin biopolymer containing titanium dioxide nanoparticles and saffron extract for use in food packaging

نویسندگان English

Shamimeh Azimi-salim 1
Maryam Azizi Lalabadi 2
Milad Tavassoli 3
Mahmood Alizadeh-Sani 4
1 Student research committee, Kermanshah university of medical sciences, Kermanshah, Iran.
2 Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Student Research Committee, Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4 PhD Student, Food Health and Safety, Department of Food Health and Safety, Faculty of Health, Tehran University of Medical Sciences.
چکیده English

In this study, biodegradable packaging based on gelatin biopolymer (at a concentration of 3% w/w) containing nanoparticles of titanium dioxide (at a concentration of 1% w/w) and saffron extract (at a concentration of 2% v) by evaporation method was synthesized. In this study, physical properties (thickness, transparency, moisture content, solubility and water vapor permeability), mechanical, antimicrobial and antioxidant properties, structural and transparency properties of films were investigated. After analyzing the data, the results showed that the effect of saffron extract and titanium dioxide nanoparticles on all the studied properties was significant (P <0.05). Addition of titanium dioxide nanoparticles and saffron extract increased the thickness, improved the mechanical properties and reduced the moisture content, water vapor permeability, transparency, and solubility. Also, nanocomposite films containing titanium dioxide nanoparticles and saffron extract showed antioxidant properties (% 80%) and acceptable antimicrobial effects, especially against gram-positive Staphylococcus aureus bacteria. According to the results of this study, this type of packaging can be suggested as a suitable alternative to synthetic packaging.

کلیدواژه‌ها English

Biodegradable film
Saffron extract
Nanocomposite packaging
Titanium dioxide nanoparticles
Antioxidant properties
- منابع
1. Basumatary, I.B., et al., Biopolymer-based nanocomposite films and coatings: recent advances in shelf-life improvement of fruits and vegetables. Critical Reviews in Food Science and Nutrition, 2020: p. 1-24.
2. Jamróz, E., et al., Furcellaran nanocomposite films: The effect of nanofillers on the structural, thermal, mechanical and antimicrobial properties of biopolymer films. Carbohydrate polymers, 2020. 240: p. 116244.
3. Mohsenzadeh, M., et al., Fabrication of biocomposite films based on sodium caseinate reinforced with gellan and Persian gums and evaluation of physicomechanical and morphology properties. Food Science and Technology, 2021. 18(113): p. 187-196.
4. Ahmadi, A., et al., Functional biocompatible nanocomposite films consisting of selenium and zinc oxide nanoparticles embedded in gelatin/cellulose nanofiber matrices. International Journal of Biological Macromolecules, 2021. 175: p. 87-97.
5. Azizi-Lalabadi, M., et al., Nanocomposite films consisting of functional nanoparticles (TiO2 and ZnO) embedded in 4A-Zeolite and mixed polymer matrices (gelatin and polyvinyl alcohol). Food Research International, 2020. 137: p. 109716.
6. Sani, M.A., A. Ehsani, and M. Hashemi, Whey protein isolate/cellulose nanofibre/TiO2 nanoparticle/rosemary essential oil nanocomposite film: Its effect on microbial and sensory quality of lamb meat and growth of common foodborne pathogenic bacteria during refrigeration. International journal of food microbiology, 2017. 251: p. 8-14.
7. Azizi-Lalabadi, M., et al., Polyvinyl alcohol/gelatin nanocomposite containing ZnO, TiO2 or ZnO/TiO2 nanoparticles doped on 4A zeolite: Microbial and sensory qualities of packaged white shrimp during refrigeration. International journal of food microbiology, 2020. 312: p. 108375.
8. Kamkar, A., et al., Nanocomposite active packaging based on chitosan biopolymer loaded with nano-liposomal essential oil: Its characterizations and effects on microbial, and chemical properties of refrigerated chicken breast fillet. International Journal of Food Microbiology, 2021. 342: p. 109071.
9. Othman, S.H., Bio-nanocomposite materials for food packaging applications: types of biopolymer and nano-sized filler. Agriculture and Agricultural Science Procedia, 2014. 2: p. 296-303.
10. Jafarian, M., et al., Producing an intelligent packaging based on peach gum and curcumin to detect the fish spoilage. Food Science and Technology, 2021. 18(117): p. 171-182.
11. Khodaei, D., K. Oltrogge, and Z. Hamidi-Esfahani, Preparation and characterization of blended edible films manufactured using gelatin, tragacanth gum and, Persian gum. LWT, 2020. 117: p. 108617.
12. Farhadvand, Z., et al., Effect of edible gelatin- mint essential oil (Mentha spicata) coating on microbial, chemical and sensory characteristics of fresh chicken fillet during storage at 4 ℃. Journal of food science and technology(Iran), 2021. 18(118): p. 249-261.
13. Alizadeh-Sani, M., et al., Kinetics analysis and susceptibility coefficient of the pathogenic bacteria by titanium dioxide and zinc oxide nanoparticles. Advanced pharmaceutical bulletin, 2020. 10(1): p. 56.
14. Azizi-Lalabadi, M. and S.M. Jafari, Bio-nanocomposites of graphene with biopolymers; fabrication, properties, and applications. Advances in colloid and interface science, 2021. 292: p. 102416.
15. Razavi, S.E. and S.M. Jafari, Effect of corm age on the antioxidant, bactericidal and fungicidal activities of saffron (Crocus sativus L.) stigmas. Food Control, 2021. 130: p. 108358.
16. Popović-Djordjević, J.B., A.Ž. Kostić, and M. Kiralan, Antioxidant activities of bioactive compounds and various extracts obtained from Saffron, in Saffron. 2021, Elsevier. p. 41-97.
17. Khazaei, K.M., et al., Optimization of anthocyanin extraction from saffron petals with response surface methodology. Food Analytical Methods, 2016. 9(7): p. 1993-2001.
18. ASTM, A., E96/E96M-16. Standard test methods for water vapor transmission of materials. Annual Book of ASTM Standards; American Society for Testing and Materials: West Conshohocken, PA, USA, 2016: p. 719-725.
19. Pulla-Huillca, P.V., et al., Wettability of gelatin-based films: The effects of hydrophilic or hydrophobic plasticizers and nanoparticle loads. Journal of Food Engineering, 2021. 297: p. 110480.
20. International, A., ASTM D882-12, Standard test method for tensile properties of thin plastic sheeting. 2012: ASTM International.
21. Luo, Z., Y. Qin, and Q. Ye, Effect of nano‐TiO2‐LDPE packaging on microbiological and physicochemical quality of Pacific white shrimp during chilled storage. International Journal of Food Science & Technology, 2015. 50(7): p. 1567-1573.
22. De Dicastillo, C.L., et al., Antioxidant films based on cross-linked methyl cellulose and native Chilean berry for food packaging applications. Carbohydrate Polymers, 2016. 136: p. 1052-1060.
23. Shankar, S., et al., Preparation, characterization, and antimicrobial activity of gelatin/ZnO nanocomposite films. Food Hydrocolloids, 2015. 45: p. 264-271.
24. Lian, Z., Y. Zhang, and Y. Zhao, Nano-TiO2 particles and high hydrostatic pressure treatment for improving functionality of polyvinyl alcohol and chitosan composite films and nano-TiO2 migration from film matrix in food simulants. Innovative food science & emerging technologies, 2016. 33: p. 145-153.
25. El Miri, N., et al., Bio-nanocomposite films reinforced with cellulose nanocrystals: Rheology of film-forming solutions, transparency, water vapor barrier and tensile properties of films. Carbohydrate Polymers, 2015. 129: p. 156-167.
26. Hosseini, S.F., et al., Fabrication of bio-nanocomposite films based on fish gelatin reinforced with chitosan nanoparticles. Food hydrocolloids, 2015. 44: p. 172-182.
27. Ahmad, J., K. Deshmukh, and M.B. Hägg, Influence of TiO2 on the chemical, mechanical, and gas separation properties of polyvinyl alcohol-titanium dioxide (PVA-TiO2) nanocomposite membranes. International Journal of Polymer Analysis and Characterization, 2013. 18(4): p. 287-296.
28. He, Q., et al., Fabrication of gelatin–TiO2 nanocomposite film and its structural, antibacterial and physical properties. International Journal of Biological Macromolecules, 2016. 84: p. 153-160.
29. Wang, Q., et al., The effects of EGCG on the mechanical, bioactivities, cross-linking and release properties of gelatin film. Food chemistry, 2019. 271: p. 204-210.
30. Voon, H.C., et al., Effect of addition of halloysite nanoclay and SiO 2 nanoparticles on barrier and mechanical properties of bovine gelatin films. Food and Bioprocess Technology, 2012. 5(5): p. 1766-1774.
31. Kuang, Q., et al., Lamellar structure change of waxy corn starch during gelatinization by time-resolved synchrotron SAXS. Food hydrocolloids, 2017. 62: p. 43-48.
32. Zolfi, M., et al., The improvement of characteristics of biodegradable films made from kefiran–whey protein by nanoparticle incorporation. Carbohydrate polymers, 2014. 109: p. 118-125.
33. El-Wakil, N.A., et al., Development of wheat gluten/nanocellulose/titanium dioxide nanocomposites for active food packaging. Carbohydrate polymers, 2015. 124: p. 337-346.
34. Espitia, P.J. and C.G. Otoni, Nanotechnology and edible films for food packaging applications. Bio-based materials for food packaging, 2018: p. 125-145.
35. Ghadermazi, R., et al., Effect of various additives on the properties of the films and coatings derived from hydroxypropyl methylcellulose—A review. Food science & nutrition, 2019. 7(11): p. 3363-3377.
36. Shahbazi, Y. and M.-H. Moosavy, Physico-mechanical and antimicrobial properties of quince seed mucilage supplemented with titanium dioxide and silicon oxide nanoparticles. Nanomedicine Research Journal, 2019. 4(3): p. 157-163.
37. Zhai, X., et al., Novel colorimetric films based on starch/polyvinyl alcohol incorporated with roselle anthocyanins for fish freshness monitoring. Food Hydrocolloids, 2017. 69: p. 308-317.
38. Ezati, P. and J.-W. Rhim, pH-responsive chitosan-based film incorporated with alizarin for intelligent packaging applications. Food Hydrocolloids, 2020. 102: p. 105629.
39. Kanmani, P. and J.-W. Rhim, Physical, mechanical and antimicrobial properties of gelatin based active nanocomposite films containing AgNPs and nanoclay. Food Hydrocolloids, 2014. 35: p. 644-652.
40. Azizi-Lalabadi, M., et al., Nanoparticles and zeolites: Antibacterial effects and their mechanism against pathogens. Current pharmaceutical biotechnology, 2019. 20(13): p. 1074-1086.
41. Ando, H., et al., Biodegradation of a poly (ε-caprolactone-co-l-lactide)–visible-light-sensitive TiO2 composite with an on/off biodegradation function. Polymer Degradation and Stability, 2015. 114: p. 65-71.
42. Slavin, Y.N., et al., Metal nanoparticles: understanding the mechanisms behind antibacterial activity. Journal of nanobiotechnology, 2017. 15(1): p. 1-20.
43. Paidari, S. and H. Ahari, The effects of nanosilver and nanoclay nanocomposites on shrimp (Penaeus semisulcatus) samples inoculated to food pathogens. Journal of Food Measurement and Characterization, 2021: p. 1-12.
44. Efatian, H., et al., Fabrication and characterization of LDPE/silver-copper/titanium dioxide nanocomposite films for application in Nile Tilapia (Oreochromis niloticus) packaging. Journal of Food Measurement and Characterization, 2021. 15(3): p. 2430-2439.
45. Zhang, X., et al., Development of multifunctional food packaging films based on chitosan, TiO2 nanoparticles and anthocyanin-rich black plum peel extract. Food hydrocolloids, 2019. 94: p. 80-92.
46. Yong, H., et al., Development of antioxidant and intelligent pH-sensing packaging films by incorporating purple-fleshed sweet potato extract into chitosan matrix. Food Hydrocolloids, 2019. 90: p. 216-224.