بررسی خواص آنتی‌اکسیدانی محدوده‌های مختلف وزن مولکولی پروتئین آبکافتی حاصل از سر ماهی کپور معمولی (Cyprinus carpio)

نویسندگان
دانشگاه علوم کشاورزی و منابع طبیعی ساری
چکیده
در مطالعه حاضر خواص آنتی­اکسیدانی محدوده­های مختلف وزن مولکولی و غلظت­های مختلف پروتئین آبکافتی حاصل از سر ماهی کپور معمولی (Cyprinus carpio) مورد بررسی قرار گرفت. ابتدا آبکافت سر ماهی کپور معمولی با آنزیم آلکالاز (غلظت 1 درصد حجمی/وزنی) در دمای 55 درجه سانتی­گراد و pH 8 در زمان­ 180 دقیقه انجام شد. سپس این پروتئین­ آبکافتی با استفاده از اولترافیلترهای 3 و 10 کیلودالتون به سه محدوده وزن مولکولی کمتر از 3، بین 3 تا 10 و بیشتر از 10 کیلودالتون تقسیم گردید. نتایج نشان داد که در فعالیت مهار رادیکال DPPH بین محدوده­های مختلف وزن مولکولی اختلاف معنی­دار وجود داشت و بیشترین و کمترین میزان به­ترتیب در وزن مولکولی 3-10 و کمتر از 3 کیلودالتون مشاهده شد (05/0p<). همچنین کمترین مقدار IC50 در مهار رادیکال DPPH برای محدوده وزن مولکولی 3-10 کیلودالتون بدست آمد (015/0±19/1میلی­گرم/میلی­لیتر). قدرت کاهندگی آهن سه ظرفیتی با افزایش غلظت، در تمامی وزن­های مولکولی افزایش یافت. همچنین بالاترین قدرت کاهندگی یون آهن در محدوده وزن مولکولی بالاتر از 10 کیلودالتون مشاهده شد (05/0p<). قدرت مهارکنندگی رادیکال ABTS، در تمامی غلظت­ها بین وزن­های مختلف مولکولی تفاوت معنی­داری نشان داد و بیشترین و کمترین میزان به­ترتیب در وزن مولکولی 3-10 و کمتر از 3 کیلودالتون مشاهده شد (05/0p<). کمترین مقدار IC50 در مهار رادیکال ABTS برای محدوده وزن مولکولی 3 تا 10 کیلودالتون بدست آمد (01/0±1/1 میلی­گرم/میلی­لیتر). به طور کلی، با توجه به تاثیر مثبت پروتئین آبکافتی سر ماهی کپور معمولی در حذف رادیکال­های آزاد DPPH، ABTS و قدرت کاهندگی آهن سه ظرفیتی در غلظت­ها و وزن­های مولکولی مختلف، می­توان اظهار داشت که این پروتئین آبکافتی می­تواند به عنوان یک آنتی­اکسیدان طبیعی، به منظور کاربرد در صنایع غذایی و یا خوراک دام، طیور و آبزیان مورد توجه قرار گیرد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

An investigation on antioxidative properties of different molecular weight fractions of protein hydrolysate produced from Common carp (Cyprinus carpio) head

نویسندگان English

Sakineh Yeganeh
Mina Esmaeili Kharyeki
Hamed Ahmadi
Sari Agricultural Sciences and Natural Resources University
چکیده English

In the present study, the antioxidant properties of different molecular weight fractions and different concentrations of protein hydrolysate from Common carp (Cyprinus carpio) head were investigated. Fish heads were hydrolyzed by Alcalase enzyme (1% v/w) at 55˚C and pH 8 during 180 min. The supernatant was fractionated to three different molecular weight fractions of <3, between 3 and 10 and >10 KDa by ultrafiltration. The results showed a significant difference between DPPH radical scavenging activity of the different molecular weight fractions, and the highest and the lowest values ​​were observed in the fractions of 3-10 and less than 3 KDa, respectively (p <0.05). The fraction with molecular weight of 3-10 KDa showed the lowest IC50 (1.15±0.015 mg/ml) for DPPH scavenging activity. As the concentration increased, ferric ion reducing power of all molecular weight fractions were increased, and the highest value was observed in the molecular weight of more than 10 kDa (p <0.05). Significant differences of ABTS radical scavenging activity were observed between the different molecular weight fractions at all concentrations, and the highest and the lowest values ​​were observed in the molecular weight fractions of 3-10 and less than 3 kDa, respectively (p <0.05). The molecular weight fraction of 3-10 kDa, exhibited the lowest IC50 value for ABTS radical scavenging activity (1.1±0.01 mg/ml). In general, according to the positive function of Common carp head protein hydrolysate at different concentrations and different molecular weights on DPPH and ABTS radical scavenging activity and ferric ion reducing power, it can be stated that this protein hydrolysate can be considered as a natural antioxidant for use in the food industry or animal, poultry and aquatic feed.

کلیدواژه‌ها English

Protein hydrolysate
Molecular weight
Common carp head
antioxidative properties
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