بررسی قدرت آنتی‌اکسیدانی و اثر ضدمیکروبی عصاره پنیرک (Malva sylvestris L.) در کلوچه سنتی لاهیجان (بر پایه آرد گندم-ارزن)

نویسندگان
1 دانشگاه فردوسی مشهد، دانشکده کشاورزی، گروه علوم و صنایع غذایی
2 استادیار گروه مهندسی علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه زابل، کد پستی 9861335856.
چکیده
ارزن منبع غنی از اسیدهای آمینه ضروری، ویتامین‏ها و مواد معدنی است. همچنین عصاره پنیرک دارای ترکیبات فنولی، خواص آنتی‏اکسیدانی و ضدمیکروبی است. هدف از بخش اول این پژوهش بررسی اثر جایگزینی بخشی از آرد گندم (صفر، 15، 30، 45 و 60 درصد) با ارزن در کلوچه بود. در این بخش رطوبت، حجم مخصوص، تخلخل و بافت ارزیابی شد. در بخش دوم به منظور تولید کلوچه فراسودمند، فعالیت آنتی‏اکسیدانی، تغییرات کپک و مخمر و ویژگی‏های حسی نمونه منتخب تحت تأثیر غلظت‏ صفر، 100، 200، 300، 400 و 500 پی‏پی‏ام عصاره پنیرک قرار گرفت و با نمونه شاهد بخش اول (مجموعا 7 نمونه) مقایسه شد. نتایج بخش اول نشان داد نمونه حاوی 15 درصد آرد ارزن به عنوان نمونه برتر بخش اول با حجم مخصوص 73/1 سانتی‏متر مکعب بر گرم، تخلخل 1/22 درصد و رطوبت 15/10 درصد و سفتی بافت 28/3 نیوتن طی دوماه نگهداری بیشترین شباهت را با شاهد داشت. نتایج بخش دوم نیز حاکی از آن بود که با افزایش عصاره در فرمولاسیون، میزان فنول کل از 6/1 به 5/53 میلی‏گرم در گرم ماده خشک رسید. این حالی بود که قدرت آنتی‏اکسیدانی نمونه حاوی 500 پی‏پی‏ام عصاره در مقایسه با شاهد از 1/12 به 2/61 درصد رسید. لازم به ذکر است در نمونه‏های حاوی بیش از 200 پی‏پی‏ام عصاره پنیرک هیچگونه کپک و مخمری طی دو ماه نگهداری مشاهده نگردید. همچنین در ارزیابی حسی نمونه‏های حاوی 100 تا 300 پی‏پی‏ام بیشترین امتیاز رنگ، بافت، بو و مزه کسب نمودند. بنابراین می‏توان نمونه حاوی 85 درصد آرد گندم، 15 درصد آرد ارزن و 300 پی‏پی‏ام عصاره پنیرک را به عنوان یک کلوچه فراسودمند به بازار محصولات نانوایی پیشنهاد نمود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Evaluation of antioxidant activity and antimicrobial effect of Malva extract (Malva sylvestris L.) in traditional Lahijan cookies (based on wheat-millet flour)

نویسندگان English

Fatemeh Pourhaji 1
Bahareh sahraiyan 1
Gholam Hossein Haghayegh 2
1 Food Science & Technology, Department of Food Science & Technology, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Food Science and Technology, Agricultural Faculty, University of Zabol, Zabol, Iran, P.O.Box: 9861335856
چکیده English

Millet is a rich source of essential amino acids, vitamins and minerals. Also, Malva extract has phenolic compounds, antioxidant and antimicrobial properties. The purpose of the first part of this study was to investigate the effect of replacing part of wheat flour (0, 15, 30, 45 and 60%) with millet flour in cookies. In this section, technological characteristics (moisture, specific volume, porosity and texture) were evaluated. In the second part, in order to produce functional cookies, antioxidant and microbial activity and sensory properties of the selected sample in the first part were affected by 0, 100, 200, 300, 400 and 500 ppm of Malva extract. The results showed that the sample containing 15% of millet flour was the best cookie of the first part with the highest specific volume (1.73 cm3 / g), porosity (22.1%) and moisture (10.15%) and favorable texture (3.28 N) during two months of storage. The results of the second part showed the total phenol content increased from 1.6 to 53.5 mg / g by increasing Malva extract in the cookie formulation. However, the antioxidant activity of the sample containing 500 ppm of Malva extract increased from 12.1% to 61.2% compared to the control. The samples containing more than 200 ppm of Malva extract didn’t have mold and yeast during two months of storage. Also, the samples containing 100 to 300 ppm Malva extract obtained the highest color, texture, odor and taste score in the sensory evaluation. Therefore, samples containing 85% wheat flour, 15% millet flour and 300 ppm of Malva extract can be offered to the bakery industry as a functional cookie.

کلیدواژه‌ها English

Functional cookie
millet
Malva extract
Antioxidant
Antimicrobial activity
[1] Park, J., CHIO, I. and Kim, Y. (2015). Cookies formulated from fresh Okara using starch, soy flour and hydroxypropyl methylcellulose have high quality and nutritional value. LWT-Food Science and Technology. 63: 660-666.
[2] Chhavi, A. & Sarita, S. (2012). Evaluation of composite millet breads for sensory and nutritional qualities and gly-cemicresponse. Malaysian journal of Nutrition, 18 (1): 89-101.
[3] Naas, (2012). Integration of millets in fortified foods, National Academy of Agricultural Sciences, New Delhi. Policy Paper NO. 54: 15P.
[4] Chappalwar, V. M., Peter, D., Bobde, H. & John, S. M. (2013). Quality characteristics of cookies prepared from oats and finger millet based composite flour. IRACST-Engineering Science and Technology: An International Journal (ESTIJ), 3: 677-683.
[5] Beswa, D. (2008).Assessment of the feasibility of using a wheat-finger millet composite flour for bread making, Submitted in fulfillment of requirement for the degree master technology in food technology in the faculty of science at the university of Johannesburg.
[6] Subedi, I., Harnisch, C., Kater, G. & Adhikari, T. (2012). Millet (Eleusinecoracana) flour fortification in composite bread. Journal of Food Science and Technology. Nepal, 7: 86-89.
[7] Iordachescu, G., Neagu, C. & Costea, T. (2013). Sensory evaluation of functional bread obtained rice and millet flour basis. Inside Food Symposium.
[8] Karuppasamy, P., Malathi, D., Banumath, P., Varadharaju, N. & Seetharaman, K. (2013). Evaluation of quality characteristics of bread from kodolittle and foxtail millets. International Journal of Food and Nutritional Sciences, 2: 35-39.
[9] Mamata, M., Nirmala, Y. &Valerie, O. (2015). Quality evaluation of little millet (Panicum miliare) incorporated functional bread. Journal of Food Science and Technology. 52(12): 8357-8363.
[10] Iva, B., Marian, T., Jan, M., Ludek, H., Oldrich, F. & Viera, S. (2017). The comparison of the effect of added amaranth, buckwheat, chickpea, corn, millet and quinoa flour on rice dough rheological characteristics, textural and sensory quality of bread. Journal of Cereal Science, 75: 158-164.
[11] Devani, B. M., Jani, B. L., Mansukhal, B. K. and Devendra, M. (2016). Study on quality of white bread enriched with finger millet flour. International Journal of Agriculture, Environment and Biotechnology, 9(5): 903-907.
[12] Vijayakumar, T.P. & Mohankumar, J. B. (2009).Formulation and characterization of millet flour blend incorporated composition flour. International Journal of Agriculture Sciences, 1: 46-54.
[13] Fathi, B., Aalami, M., Kashaninezhad, M. and Sadeghi Mahoonak, A. (2016). Utilization of the heat moisture treated Proso millet flour in production of gluten-free pound cake. Journal of food quality, 39: 611-619.
[14] Shaabani, S. Yarmand, M. S., Kiani, H. and Emam-Djomeh, Z. (2018). Protein isolate in combination with transglutaminase and xanthan on the physical and rheological characteristics of gluten-free muffins and batter based on millet flour. LWT, 90: 362-372.
[15] Chandi, G. K., Lok, C. W., Jie, N. Y. and Seetharaman, K. (2015). Functionality of kamut and millet flours in macro wire cut cookie systems. Journal of Food Science and Technology, 52(1): 556-561.
[16] Patel, P. and Thorat, S. S. (2019). Studies on chemical, textural and color Characteristics of decorticated finger millet (Eleusine coracana) fortified cake. The Pharma Innovation Journal, 8(3): 64-67.
[17] Riedl, I. (1976). Malvaceae. In: Rechinger, K. H., editor. Flora Iranica. Graz: Akad.Druck-und Verlag-Anst.
[18] Pakravan, M. (2006). New findings of genus Malva L. (Malvaceae) in Iran. Iranian Journal of Botany, 11(2): 247-249[In Persian].
[19] Lust, J. (1974). The Herb Book: The most complete catalog of herbs ever published. 1st ed. Toronto: Courier Corporation.
[20] Henry, A.G. and Piperno, D. R. (2008). Using plant microfossils from dental calculus to recover human diet: a case study from Tell al-Raqa. I, Syria. Journal of Archaeology Science, 35: 1943-1950.
[21] Tabaraki, R., Yosefi, Z. Asadi Gharneh, H. A. (2012). Chemical composition and antioxidant properties of Malva sylvestris L. Journal of Agriculture Research, 8(1): 59-68.
[22] Scherrer, A.M. Motti, R. and Weckerle, C. S. (2005). Traditional plant use in the areas of Monte Vesole and Ascea, Cilento National Park (Campania, Southern Italy). Journal of Ethno pharmacology. 97(1): 129-143.
[23] Kultur, S. Medicinal plants used in Kirklareli province (Turkey). (2007). Journal of Ethno pharmacology. 111(2): 341-364.
[24] Taha Nezhad, M., Barzegar, M., Sahari, M. A. and Naghdi Badi, H. A. (2012). Evaluation antiradical activity of Mallo (Malva sylvestris L.) extracts and its application in the oil system. Iranian Journal of Med Plants, 11(42): 86-97 [In Persian].
[25] Arzi, A., Nazari Khorasgani, Z. and Rahmani, M. (2013). Study the effects of Malva sylvestris hydro-alcoholic extract on carrageenan-induced inflammation in made rat paw. Jentashapir, 4(1): 1-10.
[26] Cleverson Gasparettoa, J., Ferreira Martinsa, C. A., Hayashia, S. S., Feleith Otukyb, M. and Pontaroloa, R. (2012). Ethno botanical and scientific aspects of Malva sylvestris L.: a millennial herbal medicine. Journal of Pharma Pharmacol, 64(2): 172-189.
[27] Marouane, W., Soussi, A., Murat, J. Bezzine, S. and El Feki, A. (2011). The protective effect of Malva sylvestris L. on rat kidney damaged by vanadium. Journal of Lipids Heath Disease, 10: 65-70.
[28] Mohammad Eini, A., Shayegh, J. and Moharrami Fard, M. (2014). Comparison of antibacterial effect of Malva sylvestris L. (Aerial and Root Oranges) by MIC. Journal of Shaheed Sadoughi University Medical Science, 21(6): 816-822 [In Persian].
[29] Basaran, D. and Ahmet, G. Antimicrobial activity of certain plants used in Turkish traditional medicine. Asian Journal of Plant Science. 3(1): 104-107 [In Persian].
[30] Alizadeh Behbahani. B., Tabatabaei Yazdi, F., Mortazavi, S. A., Zendeboodi, F., Gholian, M. M. and Vasiee, A. (2013). Effect of aqueous and ethanolic extract of Eucalyptus camaldulensis L. on food infection and intoxication microorganisms “in vitro”. Journal of Paramedical Science, 4(3):89-99.
[31] Azarhoosh, Kh., Sharifi, A. and Estiri, S. H. (2017). The effect of sugar substitution with wild persimmon syrup on antioxidant, phenolic component and sensory properties of functional cookie. Journal of Innovation in Food Science and Technology, 9(1): 123-131[In Persian].
[32] AACC. (2000). Approved Methods of the American Association of Cereal Chemists, 10th Ed., Vol. 2. American Association of Cereal Chemists, St. Paul, MN.
[33] Sabanis, D., Tzia, C. and Papadakis, S. (2008). Effect of different raisin juice preparations on selected properties of gluten-free bread. Food and Bioprocess Technology, 1(4): 374-383.
[34] Bárcenas, M. E. and Rosell, C. M. )2006(. Different approaches for improving the quality and extending the shelf life of the partially baked bread: Low temperatures and HPMC addition. Journal of Food Engineering, 72: 92–99.
[35] Kooshali Safari, Z., Ghotbi, M. and Nasirani Roozbeh, L. (2018). The effect of flour substitution with grapefruit fiber on physicochemical and sensory characteristics of cookie. Journal of Innovation in Food Science and Technology, 11(4):37-49 [In Persian].
[36] Ordoeez, A. A., Gomez, J. D. Vattuone, M. A. and Isla, M. I. (2006). Antioxidant activates of sechium edule (Jacq) swarts extracts. Food Chemistry, 97: 452-458.
[37] Burits, M. and Bucar, F. (2000). Antioxidant activity of Nigella sativa essential oil. Phytotheraphy Research, 14,pp: 323-323.
[38] Nobors, L. (2000). Sweet choices: Sugar replacement for foods and beverages. Food Technology, 56: 28-35.
[39] Sahraiyan, B., Naghipour, F., Karimi, M. & Ghiafeh davoodi, M. (2013). Evaluation of Lepidium sativum seed and guar gum to improve dough rheology and quality parameters in composite rice-wheat bread. Food Hydrocolloid. 30: 698-703.
[40] Del Vecchio, A. J. (1975). Emulsifiers and their use in soft wheat products. Bakers Digest, 49(4): 28-36.
[41] Maria, P., Massimiliano, R., Martina, C., Francesca, F., and Emma C. (2016). Chestnut flour addition in commercial gluten-free bread: A shelf-life study. LWT – food science and technology, 70: 88-95.
[42]Unver, A. Arsalan, D., Ozcan, M. M. and Akbulut, M. (2009). Phenolic content and antioxidant activity of some spices. World Appl. Science. Journal, 6: 373-377.
[43] Farina, A. Doldo, A. Cotichini, V. Rajevic, M., Quaglia, M. G. and Mulinacci, N. (1995). HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L. a comparison with gradient-elution revered-phase hplc, Jornal of Pharm Biomed Anal, 14(1-2): 203-2012.
[44] Zargari, A. [Medicinal plants]. (1995). 5th ed. Tehran: Tehran University: 34-38 [In Persian].
[45] Mustafa, A. and Ali, M. (2011). New steroidal lactones and homomonoterpenic glucoside from fruits of Malva sylvestris L. Journal of Acta Pol Pharm, 68(3): 393-401.
[46] Shokrollahi, Sh. And Heshmati, Gh. A. (2016). Different aspects of Mallow (Malva sylvestris) and results of new research finding: A Review. Journal of Neyshabur University of Medical Science, 4(1): 1-8 [In Persian].
[47] Gandomi, H., Misaghi, A., Akondzadeh, A., Bolaei, S., Khosravi, A., Abbasifar, A. and Jabelli, A. 2009. Effect of ZatariamultifloraBolss, Essential oil on growth and aflatoxin formation by Aspergillus Flavus in culture media and cheese. Journal of Food and Chemical Toxicology, 47: 2397-2400.
[48] Walter, C., Shinwari, Z. K., Afzal, I. and Malik, R. N. (2011). Antibacterial activity in herbal products used in Pakistan. Pak J Bot. 43:155-62.
[49] Dulger, B. and Gonuz A. (2004). Antimicrobial activity of certain plants used in Turkish traditional medicine. Asian Journal of Plant Science, 3(1):104-7.
[50] Islam M, Ali E, Saeed MA, Jamshaid M, Khan MTJ. Antimicrobial and Irritant activities of the extracts of Malva Parviflora L., Malvastrum Coromandelianum L. and Amaranthus viridis L.–A Preliminary Investigation. Pak J Pharmacy. 2010;20:3-6.
[51] Hayder, N., Bouhlel, I., Skandrani, I., Kadri, M., Steiman, R. and Guiraud, P. (2008). In vitro antioxidant and antigenotoxic potentials of myricetin-3-o-galactoside and myricetin-3-o-rhamnoside from Myrtus communis: modulation of expression of genes involved in cell defence system using cDNA microarray. Toxicology in vitro, 22(3):567-81.
[52] Cakir, A., Kordali, S., Zengin, H., Izumi, S. and Hirata, T. (2004). Composition and antifungal activity of essential oils isolated from Hypericum hyssopifolium and Hypericum heterophyllum. Flavour and Fragrance Journal. 19(1):62-8.
[53] Leon, K., Mery, D., Pedreschi, F. and Leon, J. (2006). Color measurement in L* a*b* units from RGB digital images. Food Research International, 39: 1084-1091.
[54] Pedreschi, F., Leon, J., Mery, D. and Moyano, P. (2006). Development of a computer vision system to measure the color of potato chips. Food Research International, 39: 1092-1098.
[55] Romani, S., Rocculi, P., Mendoza, F. and Rosa, M. D. (2009). Image characterization of potato chip appearance during frying. Journal of Food Engineering, 93: 487-494.
[56] Sahin, S. and Sumnu, S. G. (2010). Advances in deep-fat drying of foods. CRC press, new York.