بررسی اثر آنتی‌اکسیدانی و ضد میکروبی اسانس آویشن بر پنیر خامه‌ای

نویسندگان
1 استادیار گروه علوم و صنایع غذایی دانشگاه آزاد اسلامی واحد زرین دشت
2 دانشجوی کارشناسی ارشد گروه علوم و صنایع غذایی دانشگاه آزاد اسلامی واحد سروستان
چکیده
پنیر، فرآورده لبنی است که به آلودگی توسط میکروارگانیسم­های بیماریزا و فسادزا حساس است. آلودگی میکروبی می­تواند باعث کاهش عمر مفید آن و ایجاد خطرات جدی برای سلامت مصرف کنندگان شود. اسانس آویشن فعالیت آنتی­اکسیدانی و ضد میکروبی بالایی دارد و می­تواند منجر به بهبود و ارتقای ماندگاری سیستم­های غذایی گردد. به منظور بررسی تأثیر اسانس آویشن بر خصوصیات فیزیکوشیمیایی، میکروبی و حسی پنیر خامه­ای، غلظت­های مختلف اسانس آویشن (0، 005/0، 01/ و 015/0 درصد) به پنیر خامه­ای افزوده شد. نمونه های پنیر در هفته های اول، چهارم و هفتم از دوره نگهداری مورد آنالیز قرار گرفتند. نتایج نشان داد که تغییرات درصد اسانس بر میزان اسیدیته، ماده خشک، نیروی چسبندگی، IC50 و پراکسید پنیر تأثیر معنی­دار (05/0P<) دارد. با افزایش درصد اسانس روند کاهشی در میزان اسیدیته، نیروی چسبندگی، پراکسید و IC50 پنیر مشاهده شد. تغییرات درصد اسانس بر میزان pH، سختی بافت، میزان چربی و پروتئین پنیر تأثیر معنی­دار ندارد. نتایج نشان داد که زمان تأثیر معنی­داری (05/0P<) بر میزان اسیدیته، pH، ماده خشک، IC50، پراکسید و نیروی چسبندگی پنیر دارد در حالی که بر پروتئین، چربی و سختی بافت تأثیر معنی­داری نداشت. در طی دوره نگهداری اسیدیته، ماده خشک، پراکسید و IC50 افزایش و pH و چسبندگی کاهش یافتند. افزودن اسانس به پنیر تأثیر معنی­داری (05/0P<) بر تعداد کلی­فرم­ها نداشت اما موجب کاهش معنی­دار تعداد کپک و مخمر و شمارش کلی گردید. نتایج نشان داد که افزودن اسانس به پنیر بر رنگ و بافت تأثیر معنی­دار(05/0P<) ندارد، اما بر طعم و پذیرش کلی پنیر تأثیر معنی­داراست و موجب بهبود طعم و پذیرش کلی گردید. درصدهای بالای اسانس موجب کاهش امتیاز خصوصیات حسی پنیر گردید. در مجموع تیمار حاوی 005/0 درصد اسانس از نظر خصوصیات حسی بهترین تیمار بود.
کلیدواژه‌ها

عنوان مقاله English

Evaluation of antioxidant and antimicrobial effects of Thymus vulgaris essential oil on cream cheese

نویسندگان English

Hannan lashkari 1
Farzaneh Malek Zadeh Haghighi 2
1 Assistant Professor, Department of Food Science and Technology, zarindasht Branch,Islamic Azad University.
2 Department of Food Science and technology, Sarvestan Branch, Islamic Azad University.
چکیده English

Cheese is a dairy product that is susceptible to contamination by pathogenic and spoilage microorganisms. Microbial contamination can reduce its shelf life and Cause serious health risks to consumers. Thymus vulgaris essential oil has high antioxidant and antimicrobial activity and can improve and enhance the storage time of food systems. In order to evaluate the effect of thymus vulgaris essential oil on physicochemical, microbial and sensory properties of cream cheese, different percentages of thymus essential oil (0, 0.005, 0.01% and 0.015%) were added to cream cheese. Cheese sample were analyzed in the first, fourth and seventh weeks of storage. The results showed that Changes in essential oil percentage had significant effect (P<0.05) on acidity, dry matter, adhesiveness, IC50 and peroxide. With increasing essential oil percentage, a decrease in the level of acidity, adhesiveness, peroxide and IC50 was observed. Changes in essential oil percentage had no significant effect (P<0.05) on pH, fat, hardness and protein content of cheese. The results showed that storage period had a significant effect (p<0.05) on acidity, pH, dry matter, IC50, peroxide, adhesiveness, but had no significant effect (P<0.05) on protein, fat and hardness. During the storage time, Acidity, dry matter, protein and IC50 increased and pH and adhesiveness decreased. The addition of thymus essential oil did not have a significant effect (P<0.05) on coliforms but significantly decreased (P<0.05) mold and yeast and total count. The results showed that the addition of essential oil to cheese did not have a significant effect on color and texture but had a significant effect on taste and acceptance of cheese and improved the taste and acceptance. High percentage of essential oils reduced the sensory characteristics of cheese. In general, the treatment containing 0.005% essential oil was the best treatment for the sensory properties.

کلیدواژه‌ها English

Thymus vulgaris essential oil
Cheese
sensory
Physicochemical
Microbial
Nguefack, J., Budde, B.B., Jakobsen, M. (2004). Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lettersin Applid Microbiology, 39: 359-400.
[2] Roshani, S., Gohari Ardebili, A, Arianfar, A. (2015). Investigation on antimicrobial and antioxidant effects of Thymus vulgaris on mozzarella cheese, Journal of Research and Innovation in Food Science and Technology, 4(3): 233-246.
[3] Law, B. A. and Tamime, A. Y. (2010). Technology of cheese making. 2nd ed. Wiley-blackwell. Westmorland.UK.
[4] Abdel-Salam, A.B. and Saad, M.F. (2016). Influence of selected essential oils on some pathogenic microorganisms in white soft cheese. International Journal of ChemTech Research, 9 (12): 214-220.
[5] Ahmed Algarni, E.H. (2016). Soft Cheese supplemented whit Thyme, Cumin and Turmeric herbs to increase shelf life during storage period. Advances in Environmental Biology, 10 (12): 227-236.
[6] Rashidinejad, A., Birch, E.J. and Everett, D.W. (2016). Antioxidant activity and recovery of green tea catechins in full-fat cheese following gastrointestinal simulated digestion. J. Food Compos. Anal, 48: 13–24.
[7] Ganjali Dashti, N., Mirlohi, M., Ganjali Dashti, M., Jafari, M. and Bahreini Esfahani, N. (2015). Antioxidant Effect of Thyme Essential Oil on Oxidative Stability of Chicken Nuggets. International Journal of Food Engineering, 1 (2): 115-120.
[8] Kavas, G., Kavas, N. and Saygili, D. (2015). The effects of Thyme and Clove essential Oil for tified edible films on the Physical, chemical and microbiological characteristics of Kashar Cheese. Journal of Food Quality, 38: 405-412.
[9] Marcial, G.E. (2016). Influence of oregano essential oil on tradional Argentinean cheese elaboration: effect on latic starter cultures. Revista Argentina de Microbiología, 48 (3): 229- 235.
[10] Moro, A. (2015). Dairy matrix effect on the transference of rosemary essential oil compounds during cheese making. Journal of the Science of Food and Agriculture, 95:1507-1513.
[11] Mohammadzadeh Milani, J., Khedmati, SH., Ghorbani Hasansarai, A., Golkar, A. (2017). The Effect of Tragacanth Gum in Physicochemical and Textural Properties of Lighvan Cheese during Ripening. Journal of Research and Innovation in Food Science and Technology, 6(1): 103-114.
[12] Al.Otaibi, M. and El.Demerdash, H. (2008). Improvement of the quality and shelf life of concentrated yoghurt (labneh) by the addition of some essential oils. African Journal of Microbiology Research, 2:156-161.
[13] El-Din, H.M.F., Ghita, E.I., Badran, S.M.A., Gad, A.S. and El-Said, M.M. (2012). Total Phenolic Compounds, Radical Scavenging And Ferric Reducing Activity Of Low Fat UF-Soft Cheese Supplemented With Thyme Extract. Journal of Applied Sciences Research, 8 (4): 2335-2341.
[14] Mashak Z, Moradi B, Akhondzadeh basti A, Abasifar A, Gandomi H. (2009). Fate of Listeria monocytogenes During the Manufacturing Process of Iranian white Brined Cheese as Affected by Zataria multiflora Boiss. Essential oil. Journal of Medicinal Plants. 4 (29): 114-122
[15] Nour-Eldin, M., Hamad, F., Taha, E.M. and Mohamed, W.M. (2016). Effect of fortification palm oil with some Egyptian spices on physico-chemical composition, microbiological analysis, sensory evaluation and economic study of Tallaga-like cheese. Journal of Agroalimentary Processes and Technologies, 22 (4): 263-275.
[16] Miladi, H., Ben Slama, R., Mili, D., Zouari, S., Bakhrouf, A. and Ammar, E. (2013). Essential oil of Thymus vulgaris L. and Rosmarinus officinalis L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens. Natural Science, 5 (6): 729-739.
[17] Choobkar, N., Soltani. M., Ebrahimzadeh Mousavi, H.A., Akhonzadeh Basti. A. and Matinfar, A. 2010. Effect of Zataria multiflora Boiss essential oil on the growth of Staphylococcus aureus in the light salted fillets of silver carp (Hypophthalmichthys molitrix). Iran Journal of Fish Scince, 9 (3): 352-359.
[18] Smith, P., Stewart, J. and Fyfe, L. 2001. The poten tial appliction of plant essental oils as natrural food preservatives in soft cheese. Food Microbiology, 18: 463 - 70.
[19] Mohammadi; KH Karim; G Hanifian; SH Tarinejad; A Gasemnezhad R. 2011. Antimicrobial effect of Zataria multiflora Boiss. essential oil on Escherichia coli O157:H7 during manufacture and ripening of white brined cheese, Journal of Food Hygiene. 1(2):69-78.