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Soybean seeds are one of the major sources of vegetable oils produced on a commercial scale And the source of compounds such as isoflavones and phenolic compounds. In this study, the effect of two methods of ultrasound assisted extraction bath and probe ultrasound in two intensities (50%w, 75%w) by using three different types of solvents hexane, isopropanol and hexane:isopropanol composition and four levels of sonication time (30, 60, 90 and 120) on total phenolic compounds, alpha-tocopherol and extraction efficiency of soybean oil were investigated. The results showed that the highest oil extraction efficiency (8.41%) was obtained at 50% intensity of probe ultrasound at 120 min by using hexan solvent. The highest phenolic content (1.57 g GAE /kg soyben oil) and tocopherole (2.66 g α-TocoPherol/kg soyben oil) were obtained at 50% intensity of probe ultrasound at 120 min by using isopropanol solvent. In bath ultrasound extraction method the highest extraction efficiency (10.76%) was related to 30 min time of sonication and isopropanol:hexane (1:1) solvent. The highest phenol (1.48 g GAE /kg soyben oil) and tocopherol (1.68 g α-TocoPherol/kg soyben oil) were observed at 90 and 120 min of sonication, isopropanol and isopropanol:hexane solvent respectively. In all extraction methods the isopropanol solvent was the best solvent to extraction phenolic compounds. The results of this study showed that extraction by using bath and probe ultrasound had different effect on extraction oil and bioactive compounds of soybean. In each extraction method the amount of mentioned compounds was related to type of solvent and extraction method.
 

Keywords: bath ultrasound, prob ultrasound, phenolic compounds, tocopherol

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