Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Green synthesis of silver nanoparticles using aqueous extract of dill leaves and evaluation of its antibacterial activity

Document Type : Original Research

Authors
1 Assistant Professor, Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
Abstract
Interest in the biosynthesis of nanoparticles has increased in recent years by researchers. Nanoparticles have numerous applications in various fields. Synthesis of nanoparticles by green methods is environmentally safe and should be widely investigated, because different plants have a high ability to form these nanoparticles. In this study, the aqueous extract of dill (Anethum graveolens) leaves was used for the biosynthesis of silver nanoparticles. The antimicrobial activity of silver nanoparticles against microorganisms Erwinia amylovora, Pseudomonas syringae, Xanthomonas campestris, Salmonella typhi, Staphylococcus epidermidis and Listeria monocytogenes was investigated according to the disk diffusion agar, well diffusion agar, and minimum inhibitory concentration and bactericidal concentration methods. Increasing the concentration of silver nanoparticles from 20 to 110 mg/ml increased the diameter of the zone of inhibition from 7.75 mm to 11.17 mm in the disk diffusion agar and from 8.05 mm to 11.85 mm in the well diffusion agar method. X. campestris and P. syringae were identified as the most sensitive strains, and L. monocytogenes and S. epidermidis were identified as the most resistant strains to silver nanoparticles. The results of this study showed that dill leaf extract is capable of synthesizing silver nanoparticles and the produced nanoparticles showed a suitable antimicrobial effect on pathogenic strains in vitro.
 
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