Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Green synthesis of silver nanoparticles using dried tomato pomace and investigating its properties

Document Type : Original Article

Authors
1 Master's student, Food Science and Technology, Urmia University, Urmia, Iran
2 Professor, Food Science and Technology, Urmia University, Urmia, Iran
3 Assistant Professor, Food Science and Technology, Urmia University, Urmia, Iran
10.48311/fsct.2026.84084.0
Abstract
In this research, aqueous extract of dry tomato pomace was used as a reducing agent to make silver nanoparticles. By adding silver nitrate salt with a concentration of 25 mM to the extract, the reaction was carried out at a temperature of 65 °C and pH=11, and the color change of the solution from orange to dark brown indicated the beginning of the process of making silver nanoparticles. The characteristics of produced silver nanoparticles were investigated using UV-Vis spectroscopy, X-ray diffraction, infrared spectroscopy and scanning electron microscopy (FESEM). The results of UV-Vis spectroscopy showed that the plasmon absorption peak of silver nanoparticles is located at a wavelength of 420 nm. The X-ray diffraction pattern confirmed the formation of the crystalline structure of silver nanoparticles. Also, the analysis of FESEM images showed that the produced nanoparticles are often spherical and their size is less than 50 nm. In addition, the antibacterial properties of nanoparticles against Staphylococcus aureus and Escherichia coli bacteria were evaluated using the well method, which showed that these nanoparticles have a good inhibitory ability against the activity of these bacteria. The proposed green synthesis method has environmental advantages and can be used on a large scale to produce silver nanoparticles.
Keywords
Subjects

[1]Agarwal, H., Menon, S., Kumar, S. V., & Rajeshkumar, S. (2018). Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chemico-biological interactions, 286, 60-70.
[2]Dikshit, P. K., Kumar, J., Das, A. K., Sadhu, S., Sharma, S., Singh, S. & Kim, B. S. (2021). Green synthesis of metallic nanoparticles: Applications and limitations. Catalysts, 11(8), 902.
[3]Aksoylu Özbek, Z., Çelik, K., Günç Ergönül, P., & Hepçimen, A. Z. (2020). A promising food waste for food fortification: Characterization of dried tomato pomace and its cold pressed oil. Journal of Food Chemistry and Nanotechnology, 6(01), 2020-078.
[4]Machado, S., Grosso, J. P., Nouws, H. P. A., Albergaria, J. T., & Delerue-Matos, C. (2014). Utilization of food industry wastes for the production of zero-valent iron nanoparticles. Science of the Total Environment, 496, 233-240.
[5]Balčiūnaitienė, A., Viškelis, J., Urbonavičienė, D., & Viškelis, P. (2022). Tomatoes By-products Extracts Mediated Green Synthesis of Silver Nanoparticles and Their Application as Antimicrobial Agent. In Tato-From Cultivation to Processing Technology. IntechOpen.
[6]Alharbi, N. S., Alsubhi, N. S., & Felimban, A. I. (2022). Green synthesis of silver nanoparticles using medicinal plants: Characterization and application. Journal of Radiation Research and Applied Sciences, 15(3), 109-124.
[7]Zuorro, A., Iannone, A., Natali, S., & Lavecchia, R. (2019). Green synthesis of silver nanoparticles using bilberry and red currant waste extracts. Processes, 7(4), 193.
[8]Soto, K. M., Quezada-Cervantes, C. T., Hernández-Iturriaga, M., Luna-Bárcenas, G., Vazquez-Duhalt, R., & Mendoza, S. (2019). Fruit peels waste for the green synthesis of silver nanoparticles with antimicrobial activity against foodborne pathogens. Lwt, 103, 293-300.
[9]Vasyliev, G., Vorobyova, V., Skiba, M., & Khrokalo, L. (2020). Green synthesis of silver nanoparticles using waste products (apricot and black currant pomace) aqueous extracts and their characterization. Advances in Materials Science and Engineering, 2020, 1-11.
[10]Figueiredo, C. C. M., da Costa Gomes, A., Zibordi, L. C., Granero, F. O., Ximenes, V. F., Pavan, N. M., ... & da Silva, R. M. G. (2023). Biosynthesis of silver nanoparticles of Tribulus terrestris food supplement and evaluated antioxidant activity and collagenase, elastase and tyrosinase enzyme inhibition: In vitro and in silico approaches. Food and Bioproducts Processing, 138, 150-161.
[11]Vankudoth, S., Dharavath, S., Veera, S., Maduru, N., Chada, R., Chirumamilla, P., ... & Taduri, S. (2022). Green synthesis, characterization, photoluminescence and biological studies of silver nanoparticles from the leaf extract of Muntingia calabura. Biochemical and Biophysical Research Communications, 630, 143-150.
[12]Asimuddin, M., Shaik, M. R., Adil, S. F., Siddiqui, M. R. H., Alwarthan, A., Jamil, K., & Khan, M. (2020). Azadirachta indica based biosynthesis of silver nanoparticles and evaluation of their antibacterial and cytotoxic effects. Journal of King Saud University-Science, 32(1), 648-656.
[13]Ibrahim, H. M. (2015). Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms. Journal of radiation research and applied sciences, 8(3), 265-275.
[14]Khalil, M. M., Ismail, E. H., El-Baghdady, K. Z., & Mohamed, D. (2014). Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian Journal of chemistry, 7(6), 1131-1139.
[15]Kredy, H. M. (2018). The effect of pH, temperature on the green synthesis and biochemical activities of silver nanoparticles from Lawsonia inermis extract. Journal of Pharmaceutical Sciences and Research, 10(8), 2022-2026.
[16]Alafandi, L., Nasaruddin, R. R., Engliman, S., & Mastuli, M. S. (2021). Green synthesis of silver nanoparticles using coffee extract for catalysis. Malaysian NANO-An International Journal, 1(2), 13-25.
[17]Verma, A., & Mehata, M. S. (2016). Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity. Journal of radiation Research and applied sciences, 9(1), 109-115.
[18]Stavinskaya, O., Laguta, I., Fesenko, T., & Krumova, M. (2019). Effect of temperature on green synthesis of silver nanoparticles using Vitex agnus-castus extract. Chemistry Journal of Moldova, 14(2), 117-121.
[19]Birla, S. S., Gaikwad, S. C., Gade, A. K., & Rai, M. K. (2013). Rapid synthesis of silver nanoparticles from Fusarium oxysporum by optimizing physicocultural conditions. The Scientific World Journal, 2013(1), 796018.
[20]Heydari, R., & Rashidipour, M. (2015). Green synthesis of silver nanoparticles using extract of oak fruit hull (Jaft): synthesis and in vitro cytotoxic effect on MCF7 cells. International journal of breast cancer, 2015(1), 846743.
[21]Nahar, K., Rahaman, M., Khan, G. M. A., Islam, M., & Al-Reza, S. M. (2021). Green synthesis of silver nanoparticles from Citrus sinensis peel extract and its antibacterial potential. Asian Journal of Green Chemistry, 5(1), 135-150.
[22]Riaz, M., Mutreja, V., Sareen, S., Ahmad, B., Faheem, M., Zahid, N., ... & Park, J. (2021). Exceptional antibacterial and cytotoxic potency of monodisperse greener AgNPs prepared under optimized pH and temperature. Scientific Reports, 11(1), 2866.
[23]Zivyar, N., Bagherzade, G., Moudi, M., & Manzari Tavakoli, M. (2021). Evaluation of the green synthesis, characterization and antibacterial activity of silver nanoparticles from corm extract of Crocus sativus var. Haussknechtii. Journal of Horticulture and Postharvest Research, 4(Special Issue-Recent Advances in Saffron), 19-32.
[24]Bergal, A., Matar, G. H., & Andaç, M. (2022). Olive and green tea leaf extracts mediated green synthesis of silver nanoparticles (AgNPs): comparison investigation on characterizations and antibacterial activity. BioNanoScience, 12(2), 307-321.
[25]He, Y., Wei, F., Ma, Z., Zhang, H., Yang, Q., Yao, B., ... & Zhang, Q. (2017). Green synthesis of silver nanoparticles using seed extract of Alpinia katsumadai, and their antioxidant, cytotoxicity, and antibacterial activities. RSC advances, 7(63), 39842-39851.
[26]Joshi, S. J., Geetha, S. J., Al-Mamari, S., & Al-Azkawi, A. (2018). Green synthesis of silver nanoparticles using pomegranate peel extracts and its application in photocatalytic degradation of methylene blue. Jundishapur Journal of Natural Pharmaceutical Products, 13(3).
[27]Singh, M., Sinha, I., & Mandal, R. K. (2009). Role of pH in the green synthesis of silver nanoparticles. Materials Letters, 63(3-4), 425-427.
[28]Ahmad, N., & Sharma, S. (2012). Green synthesis of silver nanoparticles using extracts of Ananas comosus.
[29]Jain, S., & Mehata, M. S. (2017). Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Scientific reports, 7(1), 15867.
[30]Shaik, M. R., Khan, M., Kuniyil, M., Al-Warthan, A., Alkhathlan, H. Z., Siddiqui, M. R. H., ... & Adil, S. F. (2018). Plant-extract-assisted green synthesis of silver nanoparticles using Origanum vulgare L. extract and their microbicidal activities. Sustainability, 10(4), 913.
[31]Ahmed, S., Ahmad, M., Swami, B. L., & Ikram, S. (2016). Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of radiation research and applied sciences, 9(1), 1-7.
[32]Anandalakshmi, K., Venugobal, J., & Ramasamy, V. J. A. N. (2016). Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Applied nanoscience, 6, 399-408.
[33]Bar, H., Bhui, D. K., Sahoo, G. P., Sarkar, P., De, S. P., & Misra, A. (2009). Green synthesis of silver nanoparticles using latex of Jatropha curcas. Colloids and surfaces A: Physicochemical and engineering aspects, 339(1-3), 134-139.
[34]Balashanmugam, P., Santhosh, S., Giyaullah, H., Balakumaran, M. D., & Kalaichelvan, P. T. (2013). Mycosynthesis, characterization and antibacterial activity of silver nanoparticles from Microporus xanthopus: a macro mushroom. Int. J. Innov. Res. Sci. Eng. Technol, 2(11), 6262-6270.
[35]Palithya, S., Gaddam, S. A., Kotakadi, V. S., Penchalaneni, J., Golla, N., Krishna, S. B. N., & Naidu, C. V. (2022). Green synthesis of silver nanoparticles using flower extracts of Aerva lanata and their biomedical applications. Particulate Science and Technology, 40(1), 84-96.
[36]Akintelu, S. A., Bo, Y., & Folorunso, A. S. (2020). A review on synthesis, optimization, mechanism, characterization, and antibacterial application of silver nanoparticles synthesized from plants. Journal of Chemistry, 2020(1), 3189043.
[37]Mehata, M. S. (2021). Green route synthesis of silver nanoparticles using plants/ginger extracts with enhanced surface plasmon resonance and degradation of textile dye. Materials Science and Engineering: B, 273, 115418.
[38]Mehata, M. S. (2021). Green synthesis of silver nanoparticles using Kalanchoe pinnata leaves (life plant) and their antibacterial and photocatalytic activities. Chemical Physics Letters, 778, 138760.
[39]AlMasoud, N., Alhaik, H., Almutairi, M., Houjak, A., Hazazi, K., Alhayek, F., ... & Awad, M. A. (2021). Green nanotechnology synthesized silver nanoparticles: Characterization and testing its antibacterial activity. Green Processing and Synthesis, 10(1), 518-528.
[40] Lafta, M. Z., Al-Samarrai, R. R. H., & Bouaziz, M. (2025). Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity. Results in Chemistry, 102028.
[41] Pirsa, S. (2016). Fast determination of water content of some organic solvents by smart sensor based on PPy-Ag nanoco. Nanoscience & Nanotechnology-Asia, 6(2), 119-127.