Isolation and Identification of Lactic Microbiota from Kimchi, produced in Iran, based on Biochemical and Molecular Methods

Tabataba I Yazdi, Farideh; Vasi I, Ali Reza; Alizade Behbahani, Behrooz

Isolation and Identification of Lactic Microbiota from Kimchi, produced in Iran, based on Biochemical and Molecular Methods

Authors

Farideh Tabataba i Yazdi

Ali Reza Vasi i

Behrooz Alizade Behbahani

Abstract

Kimchi is a general term for fermented vegetables.The aim of this study was to isolate and identify the Lactic Acid Bacteria involved in spontaneous fermentation of this product used biochemical and molecular methods. In this study, after successive culture on specific media, in order to classify of 85 selected isolates that according to preliminary experiments seemed Lactic Acid Bacteria, physiological and biochemical tests were done and then fermentation of 10 different carbohydrates were performed. Based on these tests, 85 isolates were divided into 10 groups. Some isolates were selected from each group and 16S rRNA was amplified using universal primers. Diversity of lactic acid bacteria in Kimichi was as followings: Lactobacillus plantarum (41.17%), Lactobacillus fermentum (9.41%), Enterococcus faecalis (3.52%), Enterococcus faecium (7.06%), Leuconostoc citreum (2.35%), Leuconostoc mesenteroides (3.52%), Pediococcus pentosaceus (8.23%), Wisella cibaria (24.70%). This fermented product has a wide microbial diversity which originates from natural microbiota presented in the raw vegetable. Lactic acid bacteria can produce a variety of acids and enzymes which play an important role in development of unique flavor and tasteTherefore, this strains that isolated from Kimchi can be used in the food industry.

Keywords

Key words: Kimchi

Lactic flora

Biochemical tests

16s rRNA