Diversity of Lactic Acid Bacteria communities in âAsh kardeh

Tabataba I Yazdi, Farideh; Vasi I, Ali Reza; Ali Zadeh Behbahani, Behrooz; Mortazavi, Seyed Ali; Tabataba I Yazdi, Foruzan

Diversity of Lactic Acid Bacteria communities in âAsh kardeh" with using 16s rRNA gene sequence analysis and antimicrobial activity evaluation of like-bacteriocin compounds

Authors

Farideh Tabataba i Yazdi

Ali Reza Vasi i

Behrooz Ali Zadeh Behbahani

Seyed Ali Mortazavi

Foruzan Tabataba i Yazdi

Abstract

The aim of this work was identified lactic floraas a traditional fermented food and then evaluates antimicrobial activities of some strains. A total of 140 Gram-positive and catalase-negative isolates were subjected to grouping by physiological and biochemical tests and carbohydrates fermentation. Based on the resultsthese 140 isolateswere dividedinto 9 groups. Two or three isolated were selected from each group and 16S rRNA was amplified using universal primers. Diversity of lactic acid bacteria in horreh was as followings:Lactobacillus fermentum (30.00%),Lactobacillus plantarum (28.57%), Lactobacillus brevis (15.00%), Weissellacibaria(8.57%),Enterococcus (faecium and faecalis) (7.14 %), Leuconostoc (citreumand mesenteroides subsp.Mesenteroides) (6.42%) and Pediococcus pentosaceus (4.28%). Antagonistic activity of 20 isolates (strains) of lactic acid bacteriaobtained fromhorreh was evaluated against food- borne bacteria. Sixteen isolates in Agar spot method and 14 isolates in well diffusion assayshowed antibacterial activity against at least one of these indicators. Eight isolates including:Ent. faecium (1), Ent. faecalis (1), P. pentosaceus(1) and Lb. plantarum (2) exhibited the highest antagonistic activity toward Listeria innocoa. Antagonistic activity of cell free supernatant (CFS) from Lb. plantarum showed the highest thermal stability. Also, two isolates belonging to:Ent. faecium, Ent. Faecalis presented antibacterial activity at pH=7. Only, the supernatant of Lb. plantarum was not influenced by proteinase K.The results showed that the supernatant of some isolatestestedcan be used as a bio preservative in food products.

Keywords

16s rRNA

Ash kardeh

lactic acid bacteria

Antagonistic activity

Bacteriocin