Identification and quantification of anthocyanins in pomegranate peel extract.

Zare Zadeh Mehrizi, Rahmat Allah; Emam Jomeh, Zahra; Shahedi Bagh Khandan, Mohammad; Loni, Elahe; Akhavan, Hamid Reza; Biyabani, Javad

Identification and quantification of anthocyanins in pomegranate peel extract.

Authors

Rahmat Allah Zare Zadeh mehrizi

Zahra Emam Jomeh

Mohammad Shahedi Bagh Khandan

Elahe Loni

Hamid Reza Akhavan

Javad Biyabani

Abstract

In present study, dried pomegranate peel of three dominant Iranian varieties was extracted by soxhlet extraction (SE) method via four solvents. In this study Five anthocyanins were identified and quantified in the extracts by high performance liquid chromatography (HPLC) coupled with ultraviolet-visible (UV-Vis) spectrometric detector at wavelength of 517 nm according to their retention times and external standard method. According to the results extraction yield of Poost Siyahe Shirine Ardestan (PSSA) variety is more than other two varieties and extraction yield of ethanolic extracts is more than other solvents in p≤0.05. HPLC analysis was indicated pomegranate peel include of more mono glucoside anthocyanins than di glucoside anthocyanins also the most of anthocyanins particularly mono glucoside anthocyanins were detected in ethanolic extract obtained from dried peel of PSSA variety.

Keywords

HPLC

Diglucoside Anthocyanin

Acetone

Soxhelt