The probiotic, Lactobacillus acidophilus PTCC 1643 was encapsulated in calcium alginate beads with the objective of enhancing survival during exposure to the adverse conditions of the gastro-intestinal tract. The probitic was incubated in simulated gastro-intestinal conditions for 0, 30, 60, 90 and 120 min. at 37 oC. The survivability of the probiotic, L. acidophilus PTCC 1643 was expressed as the destructive value (D-value). Particle size distribution was measured using laser diffraction technique. bead appearance was observed by scanning electron microscopy (SEM). The alginate coat prevented acid-induced reduction of the probiotic in simulated gastric juice (pH 1.5, 2 h), resulting in significantly (P < 0.05) higher numbers of survivors due to retarding the permeation of the gastric fluid into the cells. After sequential incubation in simulated gastric (60 min) and intestinal juices (pH 7.25, 2 h), number of surviving cells were 6.5 log cfu ml -1 for encapsulated L. acidophilus while 2.3 log cfu ml -1 was obtained for free cells.
(2010). The influence of alginate microencapsulation on survivability of microencapsulated Lactobacillus acidophilus PTCC 1643 under simulated gastrointestinal condition. Journal of food science and technology(Iran), 7(25), 51-60.
MLA
. "The influence of alginate microencapsulation on survivability of microencapsulated Lactobacillus acidophilus PTCC 1643 under simulated gastrointestinal condition", Journal of food science and technology(Iran), 7, 25, 2010, 51-60.
HARVARD
(2010). 'The influence of alginate microencapsulation on survivability of microencapsulated Lactobacillus acidophilus PTCC 1643 under simulated gastrointestinal condition', Journal of food science and technology(Iran), 7(25), pp. 51-60.
CHICAGO
, "The influence of alginate microencapsulation on survivability of microencapsulated Lactobacillus acidophilus PTCC 1643 under simulated gastrointestinal condition," Journal of food science and technology(Iran), 7 25 (2010): 51-60,
VANCOUVER
The influence of alginate microencapsulation on survivability of microencapsulated Lactobacillus acidophilus PTCC 1643 under simulated gastrointestinal condition. Journal of food science and technology(Iran), 2010; 7(25): 51-60.
