Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Formulation of phytoniosomes from grape leaf extract (Vitis vinifera) and evaluation of its effect in fortified stirred yogurt on appetite reduction and weight loss in rat under a natural diet

Document Type : Original Article

Authors
Nahid Alidosti
Mohammad Ghorbani
Alireza Sadeghi Mahonak
hoda Shahiri Tabarestani
Gorgan University of Agricultural Sciences and Natural Resources
10.48311/fsct.2026.84094.0
Abstract
The identification of effective compounds and the anti-obesity potential of phytonisomal extract of grape leaves (genus Vitis) through appetite reduction has not been fully studied. Weight gain is known to be a risk factor for many chronic diseases, and the use of herbal supplements is one of the strategies for its management.However, limitations such as low absorption, instability, and oxidation of active compounds have challenged the application of these extracts.The use of nanocarriers such as niosomes can reduce these limitations. In this study, phytoniosomes of grape leaf extract (VLE) were prepared and their effect on appetite reduction was evaluated. The herbal extract was prepared from dried grape leaf powder of Asgari cultivar by green extraction method and its phytoniosomes were produced by thin layer hydration method.


The properties of the nanocarrier were analyzed by DLS, zeta potential and FTIR. The polyphenolic content of the extract was measured by the Folin-Ciocalty method at a wavelength of 750 nm. Finally, the effect of phytoniosomes-containing yogurt on the appetite and weight of 20 laboratory mice in four groups (control, plain yogurt, yogurt containing uncoated extract and yogurt enriched with nanocarrier containing 10 to 25% extract) was studied for six weeks. The optimized phytoniosomes formulation had an average particle size of 193.5 nm, an encapsulation efficiency of about 80%, a dispersion index of 0.37, and a zeta potential of -10.16 mV. The results showed that consumption of yogurt containing phytoniosomes led to a significant reduction in food intake and body weight in mice.
Keywords
Phytoniosome
grape leaf extract
lipid nanocarrier
fortified yogurt
appetite and weight loss
Subjects
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