Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Comparison of the Antimicrobial Effect of Hydroalcoholic Extracts of Fruit and Root of Prosopis farcta on Cariogenic Streptococci and Bacteria Causing Infection and Food Poisoning

Document Type : Original Article

Authors
1 no
2 Department of Food Science and Technology, ShQ.C., Islamic Azad University, Shahr-e Qods, Iran.
10.48311/fsct.2026.118888.83030
Abstract
Abstract
Nowadays, due to the indiscriminate use of drugs and the consequent increase in antibiotic resistance, the tendency towards the use of medicinal plants is increasing because of their fewer side effects. Most plants produce compounds called secondary metabolites that possess numerous properties, including antimicrobial effects. On the other hand, dental caries is a common oral disease, and various factors, including nutrition, influence it. In this study, the antimicrobial effects of hydroalcoholic extracts of fruit (HEPFF) and root of Prosopis farcta (HEPFR) on cariogenic Streptococci, including Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius, and Streptococcus sobrinus, as well as food poisoning bacteria (Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus) were investigated under laboratory conditions. The extracts were prepared using the ultrasound-assisted extraction method, and then their total phenolic content and total flavonoid content were determined separately. The antimicrobial properties of these extracts were evaluated by the well diffusion method and by determining (MIC) and (MBC) values. The results showed that (HEPFF) had higher total phenolic content and total flavonoid content compared to (HEPFR) (P < 0.05). Furthermore, (HEPFF) exhibited stronger antimicrobial activity against all examined strains compared to (HEPFR). Therefore, it can be concluded that (HEPFF) has desirable antimicrobial properties and can be utilized in the food and pharmaceutical industries.
Keywords
Subjects

1[- Theonodr, MR, Harold, OH, Ward, J. Swift JR. Art and science operative dentistry, 5th ed.
St. Louis Missouri: Mosby Elsevier 2006:pp: 67-70.
]2[- Hancock RE. Mechanisms of action of newer antibiotics for Gram-positive pathogens. Lancet
Infect Dis. 2005 Apr; 5(4):209-218.
]3[- Hamilton IR, Bowden GH. Oral microbiology, Encyclopedia of microbiology. San Diego: Academic Press, 2000: 466-480
]4[- Biswas S, Biswas I. Role of HtrA in surface protein expression and biofilm formation by Streptococcus mutans. Infect Immun 2005;73(10):6923-34.
]5[-Naserian R. Study of phyto chemical and antibacterial effect of Myrtus communis (Dissertation). Shiraz: Shiraz University of Medicinal Sciences; 1997. (Text in Persian)
]6[- Karimi, E., Aidy, A., & Abbasi, N. (2017). Quantitative HPLC analysis of phenolic compounds in Prosopis farcta from two different ecological zones of Iran. Chem Technol Ind J, 12(2), 116–124.
]7[- Luzhanin, V. G., Whaley, A. K., Ponkratova, A. O., Novikova, V. V., & Bezverkhniaia, E. A. (2022). Antimicrobial activity of polyphenolic compounds. Drug development & registration, 11(2), 65-72.
]8[- Dehghan, Shima, Kazemipoor, M, Mirhosseini, M, Daneshmand, F, Laboratory Comparison of the Anti-Bacterial Effect of Prosopis Farcta Extract and Hypochlorite Sodium on Enterococcus Faecalis Bacteria. (2023). Zanko journal of  medical science. 24-15
]9[- Prevete, G., Donati, E., Ruggiero, A. P., Fardellotti, S., Lilla, L., Ramundi, V., ... & Mazzonna, M. (2024). Encapsulation of Olea europaea Leaf Polyphenols in Liposomes: A Study on Their Antimicrobial Activity to Turn a Byproduct into a Tool to Treat Bacterial Infection. ACS Applied Materials & Interfaces, 16(50), 68850-68863.
 ]10[- Salari, S., Bahabadi, S.E., Samzadeh-Kermani, A., & Yosefzaei, F. (2019). In-vitro evaluation of antioxidant and antibacterial potential of green-synthesized silver nanoparticles using Prosopis farcta fruit extract. Iranian journal of pharmaceutical research: IJPR, 18(1): 430.
]11[- Mantzourani, I., Kazakos, S., Terpou, A., Mallouchos, A., Kimbaris, A., Alexopoulos, A., & Plessas, S. (2018). Assessment of volatile compounds evolution, antioxidant activity, and total phenolics content during cold storage of pomegranate beverage fermented by Lactobacillus paracasei K5. Fermentation, 4(4): 95-106.
]12[- Sharifi-Rad, J., Zhong, J., Ayatollahi, S.A., Kobarfard, F., Faizi, M., Khosravi-Dehaghi, N., & Suleria, H.A. (2021). LC-ESI-QTOF-MS/MS characterization of phenolic compounds from Prosopis farcta (Banks & Sol.) JF Macbr. And their potential antioxidant activities. Cellular and Molecular Biology, 67(1): 189-200.
 
]13[- Tsai T, Tsai T, ChienY, Lee C, Tsai P. In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus differentherbs. Food Chem. 2008;110(4):859-64.
 ]14[- Sun, W., & Shahrajabian, M. H. (2023). Therapeutic potential of phenolic compounds in medicinal plants—Natural health products for human health. Molecules, 28(4), 1845.
 ]15[- Zafari, S., & Sharifi, M. (2020). Optimization of solvent systems for the extraction of vitexin as the major bioactive flavonoid in Prosopis farcta. American Journal of Plant Sciences, 11(5), 595–603.
 ]16[-Farboodniay-Jahromi, M. A. Etemadfard, H. & Zebarjad, Z. (2018). Antimicrobial and Antioxidant Characteristics of Volatile Components and Ethanolic Fruit Extract of Prosopis farcta (Bank & Soland.). Trends Pharm Sci, 4 (3), pp. 177-186.
]17[- De Rossi, L., Rocchetti, G., Lucini, L., & Rebecchi, A. (2025). Antimicrobial potential of polyphenols: Mechanisms of action and microbial responses—A narrative review. Antioxidants, 14(2), 200.
]18[- Álvarez-Martínez, F. J., Barrajón-Catalán, E., Encinar, J. A., Rodríguez-Díaz, J. C., & Micol, V. (2020). Antimicrobial capacity of plant polyphenols against gram-positive bacteria: A comprehensive review. Current medicinal chemistry, 27(15), 2576-2606.
]19[- Alizadeh Behbahani, B., Rahmati-Joneidabad, M., Noshad, M. (2024). Ethanolic Extract of Prosopis farcta Root: Determination of Total Phenols and Flavonoids, Radical Scavenging Ability and Its Antimicrobial Effect on Some Bacteria Causing Infection and Food Poisonin. Iranian Food Science and Technology,2024,  Vol. 20, No. 1, Mar.-Apr., 2024, p. 35-46
]20[- Davidova, S., Galabov, A. S., & Satchanska, G. (2024). Antibacterial, antifungal, antiviral activity, and mechanisms of action of plant polyphenols. Microorganisms, 12(12), 2502.
 ]21[- Harzallah-Skhiri F. Jannet H. Hammami S. and Mighri Z. 2006. Variation of volatile compounds in two prosopis farcta Eig. (Fabales, Fabaceae=leguminosae populations. Flavour Frag J. 21: 484-487.
]22[- Pajohi AM, Yadollahi BM, Bazargani GB. (2016)The effect of water extract of Rhus coriaria l. on the pathogenic bacteria at different temperatures. J Babol Univ Med Sci; 18(2):417.
]23[- Salimi-Sabour E, Fattahi M, Rezaei K, Lotfali E, Khademian A. Prosopis farcta: Potent Antifungal Activity Against Trichophyton mentagrophytes Strains; A Research Based on an Ethnobotanical Study. Iran J Med Microbiol 2022; 16 (2) :127-133
]24[- Haji Ghasemi, M1, Govahi, M, Ranjbar, M. Evaluation of Antibacterial Activity of Aqueous and Hydroalcoholic Extracts of Physalis alkekengi Fruit against Four Standard Strains in vitro; Journal of Ardabil University of Medical Sciences. Vol. 22, No. 4, Winter 2023, Pages 323-332
]25[- Alizadeh Behbahani, B, Noshad, M, Mehrnia,  M, A. Investigating the antioxidant potential and antimicrobial effect of Roman (Anthemis nobilis) chamomile essential oil: “in vitro”, JFST No. 159, Vol. 22, May 2025.