Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Impact of Mixing Camel Milk with Other Species Milk on the Physiochemical Properties and Sensory Acceptability of Yogurt

Document Type : Original Article

Authors
1 Tikrit University, Faculty of Food Science / Al-Shirqat, Department of Dairy Science and Technology, 34005, Salah Al Deen, Iraq.
2 Tikrit University Faculty of Food Science Department of Dairy Science and Technology
3 Tikrit University, Faculty of Food Science / Al-Shirqat, Department of Food Science and Technology, 34005, Salah Al Deen, Iraq.
Abstract
This study was conducted during 2022–2023 in the laboratories of Tikrit and Kirkuk Universities to evaluate the possibility of manufacturing yogurt by mixing camel milk with cow, sheep, goat, and buffalo milk at ratios of 25–75% and 50–50%. The physiochemical and organoleptic properties of the produced yogurt were examined, including protein content, titratable acidity, viscosity, water retention, and whey-off over three weeks of cold storage. Statistically, camel milk blends with sheep, goat, and buffalo milk showed superior chemical characteristics, with protein values ranging between 5.25–5.48%, ash content between 0.92–0.97%, and dry matter between 11.64–13.58%. Blends of camel and cow milk exhibited reduced viscosity and a drop in pH, leading to weaker texture. Increasing camel milk levels decreased yogurt viscosity and delayed coagulation due to differences in protein composition. In contrast, camel–goat milk blends improved texture, viscosity, and product stability. Shelf-life tests showed that camel–goat and camel–buffalo blends had the best water retention and lowest whey-off compared with other treatments. Sensory evaluation indicated superior taste, texture, and overall acceptability for blends containing goat and buffalo milk, while camel–cow blends were less preferred. Sensorial properties decreased gradually during storage, with the 25–75% camel–cow blend showing relatively higher quality and stability among cow-based treatments. Overall, the findings confirm that mixing camel milk with non-bovine species, especially goat and buffalo, improves yogurt quality and strengthens its structural network compared with cow milk. Further research is recommended to enhance structural properties by incorporating natural thickening agents or fruit juices to improve texture and consumer acceptability.
Keywords

Subjects


[1]  A.O.A.C. 2023. Official Methods of Analysis,22nd., Association of Official Analytical Chemists. Inc. Virginia, USA. https://www.aoac.org/official-methods-of-analysis/
[2]  Abusheliabi, A., & Ayyash, M. (2017). Growth inhibition of foodborne pathogens in camel milk: Staphylococcus aureus, Listeria monocytogenes, Salmonella spp. and E. coli O157: H7. Czech Journal of Food Sciences, 35(4), 311. DOI: 10.17221/84/2017-CJFS 
[3]  Agustini, T. W., Soetrisnanto, D., & Ma'ruf, W. F. (2017). Study on chemical, physical, microbiological and sensory of yoghurt enriched by Spirulina platensis. International food research journal24(1). http://www.ifrj.upm.edu.my/24%20(01)%202017/(48).pdf
[4]  Ahmadoon, H. G. A. (2012). Quality changes in yoghurt made from camel milk and mixed camel milk with cow milk during preservation. Red Sea University Journal, Refereed scientific biannual Journal, 2, 24-37.
[5]  Anli, E. A., Gursel, A., Gursoy, A., & Mert, B. (2023). Assessment of the Quality Attributes of Oat β-glucan Fortified Reduced-Fat Goat Milk Yogurt Supported by Microfluidization. Foods12(18), 3457. https://doi.org/10.3390/foods12183457
[6]  Al-Hamdni, E. R., Hussein, F. F., & Jumaa, N. (2025, April). Studying The possibility of Fortifying Camel Milk with Animal Gelatin and Its Effect on the Sensory and Chemical Properties of Yogurt Made from It. In IOP Conference Series: Earth and Environmental Science (Vol. 1487, No. 1, p. 012120). Doi:10.1088/1755-1315/1487/1/012120
[7]  Ayoub, M. A., Yap, P. G., Mudgil, P., Khan, F. B., Anwar, I., Muhammad, K., et al. (2024). Invited review: Camel milk–derived bioactive peptides and diabetes—molecular view and perspectives. Journal of Dairy Science, 107(2), 649–668.https://doi.org/10.3168/JDS.2023-23733 
[8]  Ayyash, M., Al-Nuaimi, A. K., Al-Mahadin, S., & Liu, S. Q. (2018). In vitro investigation of anticancer and ACE-inhibiting activity, α-amylase and α-glucosidase inhibition, and antioxidant activity of camel milk fermented with camel milk probiotic: A comparative study with fermented bovine milk. Food chemistry, 239, 588-597.                                                                Doi: 10.1016/j.foodchem.2017.06.149 
[9]  Balthazar, C. F., Pimentel, T. C., Ferrão, L. L., Almada, C. N., Santillo, A., Albenzio, M., & Cruz, A. G. (2017). Sheep milk: Physicochemical characteristics and relevance for functional food development. Comprehensive reviews in food science and food safety, 16(2), 247-262. Doi: 10.1111/1541-4337.12250.
[10]             Bhawna S., Mustapha M., Huda A.,Alya A.,Aysha A, Muna A,Haleimahs.,Tholkappiyan R.,Fathalla H.&Afaf K.Eldin (2020); Rhedogical characteristics and consumer acceptance of camel milk yogurts as affected by bovin proteins and hydrocolloids .International Journal of food properties ,vol.23,No.1,1347-1360.http://doi.org/10.1080/10942912.2020.1797785 
[11]             Bulca, S., Dumanoğlu, B., & Özdemir, Ö.C. (2019). A Study on Mixing Camel Milk with Cow, Sheep and Goat Milk in Different Proportions in Yoghurt Production. Turkish Journal of Agriculture: Food Science and Technology, 7, 2095-2102. DOI:10.24925/turjaf.v7i12.2095-2102.2823
[12]             Choudhary, V., Bais, B., Kumar, Y., Goklaney, D., Saran, D., Kanwar, P., ... & Kumar, A. (2024). COMPARATIVE ANALYSIS OF PHYSICO-CHEMICAL PROPERTIES OF BUFFALO, CAMEL AND BLENDED (BUFFALO 70%: CAMEL 30%) MILK. Journal of Camel Practice and Research, Vol 31 No 1, p 77-81.  DOI: 10.5958/2277-8934.2024.00013.4.
[13]             Deshwal GK, Tiwari S, Kadyan S (2021) Applications of emerging processing technologies for quality and safety enhancement of non-bovine milk and milk products. Lwt 149:111845. https://doi.org/10.1016/j.lwt.2021.111845
[14]             Eissa, E. A., Yagoub, A. E. A., Babiker, E. E., & Ahmed, I. M. (2011). Physicochemical, microbiological and sensory characteristics of yoghurt produced from camel milk during storage.
[15]             El Zubeir, I. E. M., Basher, M. A. E., Alameen, M. H., Mohammed, M. A. S., & Shuiep, E. S. (2012). The processing properties, chemical characteristics and acceptability of yoghurt made from non-bovine milks. Development, 24(3), 50. http://www.lrrd.org/lrrd24/3/zube24050.htm 
[16]             El-Fakharany, E. M., Abu-Serie, M. M., Litus, E. A., Permyakov, S. E., Permyakov, E. A., Uversky, V. N., & Redwan, E. M. (2018). The use of human, bovine, and camel milk albumins in anticancer complexes with oleic acid. The Protein Journal, 37, 203-215.                           Doi: 10.1007/s10930-018-9770-1 
[17]             El-Hatmi, H., Jrad, Z., Salhi, I., Aguibi, A., Nadri, A., & Khorchani, T. (2015). Comparison of composition and whey protein fractions of human, camel, donkey, goat and cow milk. Dairy/Mljekarstvo, 65(3) 65:159–67. https://doi.org/10.15567/mljekarstvo.2015.0302
[18]             Guinee TP, Caric M, Kalab M and Hynes ER. (2015). Relationships between the composition, coagulation and rheological properties of milk from different species: a review. International Dairy Journal.; 51:1-12.
[19]             Hara, K., Ikeda, M., Saito, S., Matsumoto, S., Numata, K., Kato, N., ... & Sekihara, H. (2002). Lactoferrin inhibits hepatitis B virus infection in cultured human hepatocytes. Hepatology Research, 24(3), 228-235. Doi: 10.1016/s1386-6346(02)00088-8 
[20]             Hinz K, O'Connor PM, Huppertz T, Ross RP, Kelly AL. Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk. J Dairy Res. (2012) 79:185–91. Doi: 10.1017/S0022029912000015
[21]             Ho, T. M., Zou, Z., & Bansal, N. (2022). Camel milk: A review of its nutritional value, heat stability, and potential food products. Food Research International, 153, Article 110870. https://doi.org/10.1016/J.FOODRES.2021.110870
[22]             Ibrahim, A., Naufalin, R., Muryatmo, E., & Dwiyanti, H. (2021, April). Comparative study between cow and goat milk yogurt based on composition and sensory evaluation. In IOP Conference Series: Earth and Environmental Science (Vol. 746, No. 1, p. 012001). IOP Publishing. Doi:10.1088/1755-1315/746/1/012001.
[23]             IDF (2009). Milk and milk products - Determination of fat content - General guidance on the use of butyrometric methods. IDF 152:2009. International Dairy Federation, Brussels, Belgium. https://cdn.standards.iteh.ai/samples/53829/1966dfc8e65742ffb92f61ab7d9bae02/ISO-11870-2009.pdf
[24]             Jandal AJM. (2013). The effect of adding anhydrous acetic acid to prepare whey protein concentrates to improve its functional properties. Diyala Agric. Sci. J. (DASJ), 5(2), 10-24. https://journal.djas.uodiyala.edu.iq/index.php/dasj/article/view/2746   
[25]             Kaskous, S. (2016). Importance of camel milk for human health. Emirates Journal of Food and Agriculture, 28(3), 158-163. DOI: https://doi.org/10.9755/ejfa.2015-05-296 
[26]             Khakhariya, R., Basaiawmoit, B., Sakure, A. A., Maurya, R., Bishnoi, M., Kondepudi, K. K., et al. (2023). Production and characterization of ACE inhibitory and anti-diabetic peptides from Buffalo and camel milk fermented with Lactobacillus and yeast: A comparative analysis with in vitro, in silico, and molecular interaction study. Foods, 12(10), 2006. https://doi.org/10.3390/FOODS12102006/S1
[27]             Khalel, A. M., Salih, G. M., & Al-Janabi, A. M. (2023, December). Effect of Adding Different Concentrations of Olevera Extract on the Microbial and Sensory Characteristics of Manufactured Yogurt During Storage Periods. In IOP Conference Series: Earth and Environmental Science (Vol. 1262, No. 6, p. 062021). Doi:10.1088/1755-1315/1262/6/062021
[28]             Khalifa, M. I., & Zakaria, A. M. (2019). Physiochemical, sensory characteristics and acceptability of a new set yogurt developed from camel and goat milk mixed with buffalo milk. Anim. Vet. Sci, 7(3), 172-177.  http://dx.doi.org/10.17582/journal.aavs/2019/7.3.172.177
[29]             Khaliq, A., Mishra, A. K., Niroula, A., Baba, W. N., Shaukat, M. N., & Rabbani, A. (2024). An updated comprehensive review of camel milk: Composition, therapeutic properties, and industrial applications. Food Bioscience62, 10553.https://doi.org/10.1016/j.fbio.2024.105531 
[30]             Kiyanisefat, M. B., Sedaghati, M., & Shakouri, M. J. (2025). Assessment of the physicochemical, antioxidant, microbial, and sensory properties of camel milk fermented with Lactobacillus plantarum and Lactobacillus rhamnosus. Journal of Agricultural Science and Technology, e24103. https://jast.modares.ac.ir/article_24103_ee071bf2ba766690d86388d4a3d870a0.pdf
[31]             Kumar, D., Chatli, M. K., Singh, R., Mehta, N., & Kumar, P. (2016). Antioxidant and antimicrobial activity of camel milk casein hydrolysates and its fractions. Small Ruminant Research, 139, 20-25.  DOI: 10.1016/j.smallrumres.2016.05.002
[32]             Lee, W. J., & Lucey, J. A. (2010). Formation and physical properties of yogurt. Asian-Australasian journal of animal sciences, 23(9), 1127-1136. https://doi.org/10.5713/ajas.2010.r.05
[33]             Li, B., Ye, L., Zhao, Y., Liu, Y., Chen, Y., & Zhang, H. (2025). A comprehensive review of probiotic yogurt: Nutritional modulation, flavor improvement, health benefits, and advances in processing techniques. Agricultural Products Processing and Storage1(1), 17.
[34]                  https://doi.org/10.1007/s44462-025-00023-7
[35]             Mbye, M., Mohamed, H., Ramachandran, T., Hamed, F., AlHammadi, A., Kamleh, R., & Kamal-Eldin, A. (2021). Effects of pasteurization and high-pressure processing of camel and bovine cheese quality, and proteolysis contribution to camel cheese softness. Frontiers in Nutrition, 8, 642846.  https://doi.org/10.3389/fnut.2021.642846
[36]             Mudgil, P., Kamal, H., Yuen, G. C., & Maqsood, S. (2018). Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates. Food chemistry, 259, 46-54. Doi: 10.1016/j.foodchem.2018.03.082
[37]             Mustafa, E., Bhagiel, T., Tabidi, M., & Ahmed, M. (2015). The effect of mixing different percentages of cow milk on the physiochemical characteristics of camel milk yoghurt and the sensory evaluation of yoghurt. World J. Pharmac. Pharma. Sci4(09), 180-190. http://www.wjpps.com/wjpps_controller/abstract_id/3687
[38]             Nguyen HTH, Afsar S, Day L. Differences in the microstructure and rheological properties of low-fat yoghurts from goat, sheep and cow milk. Food Res Int. (2018) 108:423–9.                       Doi: 10.1016/j.foodres.2018.03.040 
[39]             Omar, A., Harbourne, N., & Oruna-Concha, M. J. (2018). Effects of industrial processing methods on camel skimmed milk properties. International Dairy Journal, 84, 15-22. DOI: 10.1016/j.idairyj.2018.03.011.
[40]             Pak, V. V., Khojimatov, O. K., Abdiniyazova, G. J., & Magay, E. B. (2019). Composition of camel milk and evaluation of food supply for camels in Uzbekistan. Journal of Ethnic Foods, 6(1), 1–8. https://doi.org/10.1186/S42779-019-0031-5/FIGURES/5   
[41]             SAS (2001). Statistical Analysis System. SAS Institute Inc. Cary, Nc. USA.
[42]             Tamime, A. Y. and R. K. Robinson. (1999). Yoghurt: Science and Technology. 2nd edn. CRC Press, Boca Raton, FL.
[43]             Terzioğlu, M. E., Bakırcı, İ., Oz, E., Brennan, C. S., Huppertz, T., Amarowicz, R., ... & Oz, F. (2023). Comparison of camel, buffalo, cow, goat, and sheep yoghurts in terms of various physicochemical, biochemical, textural and rheological properties. International Dairy Journal146, 105749. http://dx.doi.org/10.2139/ssrn.4431399
[44]             Wang, H., Zhang, Y., Han, Y., Hou, J., Zuo, Y., Li, Y., & Wu, H. (2025). The Physicochemical, Sensory, and Functional Properties of Yogurt Containing Millet and Milk. Foods14(20), 3491. https://doi.org/10.3390/foods14203491