Efficiency of response surface methodology to optimization ethanolic extract from Cordiaâ myxa and evaluation of antimicrobial activity of extract against a number of pathogenic microorganisms

Pirnia, Motahare; Edalatian, Mohammad Reza; Tabataba I Yazdi, Farideh; Shahidi, Fakhri

Efficiency of response surface methodology to optimization ethanolic extract from Cordiaâ myxa and evaluation of antimicrobial activity of extract against a number of pathogenic microorganisms

Authors

Motahare Pirnia

Mohammad Reza Edalatian

Farideh Tabataba i Yazdi

Fakhri Shahidi

Abstract

With emerging of infectious diseases and spread of antibiotic resistant strains, use of antimicrobial compounds with plant origin seems necessary. In this study, ethanolic extract of Cordia myxa fruit was used to evaluate antimicrobial effects against microorganisms including Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella typhi, Aspergillus niger and Candida albicans. Ultrasound-assisted extraction was performed to investigate three independent variables: time (5- 40 min), temperature (20- 50°C) and sonic power (20- 100%). Response surface methodology was also employed to optimize multiple variables to predict the best process conditions. Antimicrobial activity was done by methods including disk diffusion agar, pour-plate, minimum inhibition concentration and minimum bactericide (fungicide) concentration. The results showed that the highest amount of extraction rate of ethanolic extract which was equal to 8.5%, was obtained in extraction time of 39.8 min, temperature of 42.2°C and sonic power of 94.4%.In all above-mentioned methods, inhibitory effect of optimum ethanolic extract was more significant againstBacillus cereus, Staphylococcus aureus and Candida albicansthan other strains (p≤0.05).

Keywords

Key words: Cordia myxa

Ethanolic extract

Antimicrobial activity

response surface methodology