Efficiency of response surface methodology to optimization ethanolic extract from Cordia‌ myxa and evaluation of antimicrobial activity of extract against a number of pathogenic microorganisms

Authors
Motahare Pirnia
Mohammad Reza Edalatian
Farideh Tabataba i Yazdi
Fakhri Shahidi
Abstract
With emerging of infectious diseases and spread of antibiotic resistant strains, use of antimicrobial compounds with plant origin seems necessary. In this study, ethanolic extract of Cordia myxa fruit was used to evaluate antimicrobial effects against microorganisms including Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella typhi, Aspergillus niger and Candida albicans. Ultrasound-assisted extraction was performed to investigate three independent variables: time (5- 40 min), temperature (20- 50°C) and sonic power (20- 100%). Response surface methodology was also employed to optimize multiple variables to predict the best process conditions. Antimicrobial activity was done by methods including disk diffusion agar, pour-plate, minimum inhibition concentration and minimum bactericide (fungicide) concentration. The results showed that the highest amount of extraction rate of ethanolic extract which was equal to 8.5%, was obtained in extraction time of 39.8 min, temperature of 42.2°C and sonic power of 94.4%.In all above-mentioned methods, inhibitory effect of optimum ethanolic extract was more significant againstBacillus cereus, Staphylococcus aureus and Candida albicansthan other strains (p≤0.05).
Keywords
Key words: Cordia myxa
Ethanolic extract
Antimicrobial activity
response surface methodology
Journal of food science and technology(Iran)
Volume 13, Issue 55
September 2015
Pages 55-67