Investigation of the extracts antibacterial effect of Hibiscus Sabdariffa against strains of antibiotic resistance on pathogenic bacteria âin vitroâ

Tabataba I Yazdi, Farideh; Ali Zadeh Behbahani, Behrooz; Vasi I, Ali Reza; Mortazavi, Seyed Ali; Moradi, Samira

Investigation of the extracts antibacterial effect of Hibiscus Sabdariffa against strains of antibiotic resistance on pathogenic bacteria âin vitroâ

Authors

Farideh Tabataba i Yazdi

Behrooz Ali Zadeh Behbahani

Ali Reza Vasi i

Seyed Ali Mortazavi

Samira Moradi

Abstract

Infectious diseases created by strains of Antibiotic resistance Escherichia coli and Staphylococcus aureus, is increasing in many countries such as Iran. Therefore, many efforts are performing in order to find a new composition as replacement for antibiotics. In this experimental research, an antimicrobial effect of the ethanolic and aqueous extract of Hibiscus Sabdariffa was investigated on several strains of Escherichia coli and Staphylococcus aureus resistant to common clinical antibiotics.The extract of Hibiscus Sabdariffa was prepared using rotary. Twenty strains of Escherichia coli and Staphylococcus aureus were provided from Mashhad University of Medical Sciences. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using Serial Dilution Method in six concentrations onto strains of Escherichia coli and Staphylococcus aureus in broth media.The results showed that Escherichia coli was resistant to antibiotics of penicillin (75.9%), erythromycin (58.3%), tetracycline (56.9%), and cefixime (37%), and Staphylococcus aureus showed resistant to antibiotics of penicillin (83.5%), cefixime (80%), erythromycin (55.6%), and tetracycline (26.1%), respectively. Also, the ethanolic extract of Hibiscus Sabdariffa has acceptable effect on antibiotic resistant strains of Escherichia coli and Staphylococcus aureus that MIC of the Hibiscus Sabdariffa ethanolic extract was 4 and 16 mg/mL for Escherichia coli and Staphylococcus aureus, respectively.The overall, the Hibiscus Sabdariffa extract has inhibitory ability on Antibiotic resistant Escherichia coli and Staphylococcus aureus strains. So, the ethanolic and aqueous extract of Hibiscus tea can be used in in pharmaceutical industry for medical treatments with perfect study.

Keywords

Hibiscus Sabdariffa

Escherichia coli

Staphylococcus aureus

Antibacterial effect

Extract