Investigation of the potential of Lactobacillus sp. isolated from traditional dairy products in aflatoxin B1 elimination Tajabadi Ebrahimi, M. 1 , Bahrami, H. 2, Hashemi, M. 3, Mazaheri, M. 4, Jaafari, P. 5

Aflatoxins are among the secondary metabolites of moulds that have the most toxicity between the mycotoxins. Different physical, chemical and biological approaches have been evaluated to detoxify the aflatoxins. One of the biological methods to detoxify the aflatoxin is using beneficial microorganisms such as lactic acid bacteria to remove the toxin. A lot of studies believed that physical attachment of lactobacilli to mutagens and removing them is the main protection factor. In this study the potential of some of lactobacilli that have been isolated from traditional dairy products to absorb and remove aflatoxin B₁ was investigated through ELISA and HPLC methods. The results obtained from screening the lactobacilli through ELISA method indicated the difference potential of lactobacilli in detoxification of aflatoxin B₁. Comparison the results of ELISA and HPLC methods obtained point out that ELISA as a rapid and easy method is a suitable approach in initial screening of isolates with potential of aflatoxin B₁ detoxification but for next steps more sensitive methods such as HPLC should be used. Complementary in vitro and in vivo studies could lead to introduce Iranian native isolates that have potential to apply in related industries. Such isolates could be used in food and feed industries to detoxify the aflatoxin B₁.
بررسی توانایی سویه های لاکتوباسیل جدا شده از فرآورده­های لبنی سنتی در حذف آفلاتوکسین B₁

مریم تاج آبادی ابراهیمی¹ *، هدی بهرامی² ، مریم هاشمی³، منصوره مظاهری⁴، پروانه جعفری⁵



* مسئول مکاتبات: m.tajabadi@iauctb.ac.ir