Investigation of metabolism of Mortierella alpina in optimal condition for oil and arachidonic acid production using response surface methodology (RSM) Samad loui, H. R. 1, Hamidi, Z. 2ïª, Alavi, S. M. 3, Sahari, S. A. 4, Abbasi, S. 2

Investigation of metabolism of Mortierella alpina in optimal condition for oil and arachidonic acid production using response surface methodology (RSM) Samad loui, H. R. 1, Hamidi, Z. 2ïª, Alavi, S. M. 3, Sahari, S. A. 4, Abbasi, S. 2

Abstract

In this reasearch, optimal condition on oil and arachidonic acid production by mortierella alpina CBS 754.68 was investigated using response surface methodology. Tow factors which influencing arachidonic acid and oil production; including carbon source (glucose) in five levels (45.8, 50, 60, 70 and 74.14 gl) and nitrogen source (soybean meal) in five levels (7.92, 10, 15, 20 and 22.07 gl) were studied. Arachidonic acid and oil yield varied msignificantly in response to changes in concentration of soybean meal and glucose. The model predicted that, maximum production of AA (that is, up to %56.40) and oil (that is, up to %50.13) could be achieved using the medium containing (glucose 50.35 and soybean 18.30 gl) and (glucose 70 and soybean 10 gl), respectively. In the next stage change the medium composition and biomass during fermentation was investigated in two medium optimized. The results showed that oil and arachidonic acid production significant increase with fungi and storage oil stopped respectively. Phenolic compounds are directly related with cell growth rate and the amount of fungi oil.

Keywords

Mortierella alpine

Fermentation

Arachidonic acid

Oil

response surface methodology