Investigation of the CaMV35s gene in baby food and infant formula in Tehran by real-time PCR technique

Authors
ramin beheshti zadeh 1
shila Safaeian 1
elham moslemi 2
Kasra Esfahani 3
Rezvan Mousavi nadushan 1
1 Department of Food Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
3 Department of Plant Bioproducts, Institute of Agricultural Biotechnology (IAB), National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
10.22034/FSCT.19.133.167
Abstract
Corn and soybeans have the largest area of ​​GM crops in the world. Milk powder and baby food are processed foods that contain corn and soybeans. Therefore, tracking transgenic corn and soy in processed food is one of the research problems. First, 40 samples of baby food and milk powder were collected from pharmacies and supermarkets in Tehran. All the samples were extracted using Azmaelixir DNA kit, and the nucleic acid concentration was checked with a nanodrop device, and internal control genes for soy (Lectin) and corn (Zein) were checked to ensure the extraction. Then, the presence of CaMV35S transgenic gene was checked by Real-time PCR technique. The results showed the presence of transgenic genes in 2.5% of baby food samples and 10% of samples in infant formula. Therefore, in this article, the amount of transgenic gene penetration in baby food and infant formula has been investigated.
Keywords
Detection
Real-time PCR
GMO
Infant formula

Subjects

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