Fabrication of an amperometric aptasensor for determination of antibiotic amount residues in milk

Authors
Ayat Mohammad-Razdari 1
Mahdi Ghasemi-Varnamkhasti 2
sajad rostami 3
Ali. A Ensafi 4
Zahra Izadi 5
Mojtaba Bonyadian 6
1 Ph.D. student, Department of Mechanical Engineering Biosystems, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran
2 Associate Professor, Department of Mechanical Engineering of Biosystems,Faculty of Agriculture, Shahrekord University.
3 Associate Professor, Department of Mechanical Engineering of Biosystems, Faculty of Agriculture, Shahrekord University.
4 Professor, Department of Chemistry, Isfahan University of Technology.
5 Assistant Professor, Department of Mechanical Engineering of Biosystems, Faculty of Agriculture, Shahrekord University.
6 Associate Professor, Department of Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University
Abstract
Quantitative detection of drug residues in animal food stuffs is very important. Excessive use of veterinarian veterinarians, like antibiotics are a serious threat to consumers, due to the residence of livestock products such as meat, milk, eggs. Rapid detection of antibiotics is essential by using an efficient, fast, affordable, and specific tool for risk reduction and food safety control. In the present study, an aptasensor based on pencil graphite electrode modified with nanomaterial including grapheme and gold, for rapid detection of tetracycline antibiotic was developed in milk samples. Cycle voltammetry and differential pulse voltammetry (DPV) techniques were used for response evaluation of aptasensor. In order to modification the graphite pencil electrode, the scanrate (40 mV/s) and the number of cycles (10) and immobilization time of graphene (90 min) were optimized. Under optimum conditions, using differential pulse voltammetry technique was found to increase linearly in the range of 1 × 10-12 to 1 × 10-5 M, with increasing concentration (R² = 0.985). The detection limit of the aptasensor was found to be 1.4× 10-13 M. A review of functional characteristics including repeatability, reproducibility, satability, and selectivity suggests acceptable performance for aptasensor. Overall, the fabricated aptasensor has efficiency required to detect tetracycline in milk samples.
Keywords
Aptasensor
Amperometric Antibiotic
Nanomaterial
Differential Pulse Voltammetry (DPV)

Subjects

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Journal of food science and technology(Iran)
Volume 16, Issue 89
July 2019
Pages 47-57