Screening of effective factors in the growth of Paenibacillus polymyxa using Plackett - Burman experimental desig

Authors
Alireza Vasiee 1
Mahboobe Sarabi-Jamab 2
Fereshteh Falah 3
Seyed Ali Mortazavi 3
1 Department of Food Safety and Quality Control, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran
2 Department of Food Biotechnology, Research Institute of Food Science and Technology, RIFST, Mashhad, Iran
3 Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
10.22034/FSCT.22.158.213
Abstract
Paenibacillus polymyxa is one of the microorganisms that has the ability to produce extracellular exopolysaccharides and antibiotics. Several factors, including culture medium content, carbon and nitrogen sources, pH, temperature, air velocity, and culture conditions, have an effect on the growth and production of higher cell mass, as well as the production of microbial metabolites. The purpose of this study was to investigate the growth rate of P. polymyxa in a culture medium containing molasses and to screen five components of the culture medium along with four factors of the fermentation conditions using the Plackett -Burman method to maximize cell mass production. The results showed that among the investigated variables, molasses brix, time, percentage of inoculation, amount of ammonium sulfate, stirring speed, and the amount of glucose and urea, as a first-order equatino, had a significant positive effect on bacterial growth and biomass production. Molasses brix medium was found to be more effective than other variables; however, pH and the amount of low-use elements had a negative effect on cell growth. The findings of this study indicated that molasses-based culture medium can be used as a cost-effective and suitable option for the growth of P. polymyxa.


Keywords
Paenibacillus polymyxa
cell growth
Plackett -Burman

Subjects

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