Investigating the chemical properties and antibacterial activity of Tanacetum balsamita L. essential oil: a study in vitro

Authors
1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
Abstract
This study aimed to investigate the chemical composition, antioxidant properties, and antimicrobial activity of Tanacetum balsamita L. essential oil. The essential oil was extracted using the water distillation method, and then the total phenolic content (using the Folin-Ciocalteu method), total flavonoid content (using the aluminium chloride colorimetric method), antioxidant activity (using DPPH and ABTS free radical scavenging methods), and antimicrobial activity (using disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration methods) were evaluated. The total phenolic and flavonoid contents of the essential oil were found to be 29.50 mg gallic acid equivalent per gram and 14.90 mg quercetin equivalent per gram, respectively. The essential oil exhibited significant antioxidant activity, with the ability to scavenge 50.40% and 53.55% of free radicals DPPH and ABTS, respectively. According to the results of disc diffusion agar method, Bacillus subtilis and Salmonella typhi were the most sensitive and resistant bacterial strains, respectively, with zone of inhibition diameters of 16 mm and 10.50 mm. The zone of inhibition diameters for these bacteria in the well diffusion agar method were obtained as 16.30 mm and 10.80 mm, respectively. The minimum inhibitory concentration and minimum bactericidal concentration for B. subtilis were determined as 4 mg/mL and 32 mg/mL, respectively, and for S. typhi, they were 64 mg/mL and greater than 512 mg/mL, respectively. According to the results, T. balsamita essential oil can be used as a natural antioxidant and antimicrobial agent in food products.
Keywords

Subjects


[1] Noshad, M., Behbahani, B. A., Nikfarjam, Z., & Zargari, F. (2023). Antimicrobial activity between Coriandrum sativum seed and Cuminum cyminum essential oils against foodborne pathogens: A multi-ligand molecular docking simulation. LWT, 185, 115217. https://doi.org/https://doi.org/10.1016/j.lwt.2023.115217.
[2] Sureshjani, M. H., Yazdi, F. T., Mortazavi, S. A., Behbahani, B. A., & Shahidi, F. (2014). Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria" in vitro. Journal of Paramedical Sciences, 5(2), 115-120.
[3] Behbahani, B. A., Shahidi, F., Yazdi, F. T., & Mohebbi, M. (2013). Antifungal effect of aqueous and ethanolic mangrove plant extract on pathogenic fungus" in vitro". International Journal of Agronomy and Plant Production, 4(7), 1652-1658.
[4] Falah, F., Shirani, K., Vasiee, A., Yazdi, F. T., & Behbahani, B. A. (2021). In vitro screening of phytochemicals, antioxidant, antimicrobial, and cytotoxic activity of Echinops setifer extract. Biocatalysis and Agricultural Biotechnology, 35, 102102.
[5] Sharma, K., Guleria, S., Razdan, V. K., & Babu, V. (2020). Synergistic antioxidant and antimicrobial activities of essential oils of some selected medicinal plants in combination and with synthetic compounds. Industrial Crops and Products, 154, 112569. https://doi.org/https://doi.org/10.1016/j.indcrop.2020.112569 .
[6] Tabatabaei Yazdi, F., Alizadeh Behbahani, B., Vasiee, A., Mortazavi, S. A., & Yazdi, F. T. (2015). An investigation on the effect of alcoholic and aqueous extracts of Dorema aucheri (Bilhar) on some pathogenic bacteria in vitro. Archives of Advances in Biosciences, 6(1), 58-64.
[7] Alizadeh Behbahani, B., Yazdi, F. T., Mortazavi, A., Gholian, M. M., Zendeboodi, F., & Vasiee, A. (2014). Antimicrobial effect of Carboxy Methyl Cellulose (CMC) containing aqueous and ethanolic Eucalyptus camaldulensis L. leaves extract against Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis. Archives of Advances in Biosciences, 5(2), 59-69.
[8] Yazdi, F. T., Falah, F., Behbahani, B. A., Vasiee, A., & Mortazavi, S. A. (2019). Identification of Chemical Compounds, Antioxidant Potential, Phenolic Content and Evaluation of Inhibitory and Bactericidal/Fungicidal Effects of Ginger Essential Oil on Some Pathogenic Microorganisms in Vitro. Qom University of Medical Sciences Journal 13(3), 50-62.
[9] Alizadeh Behbahani, B., Falah, F., Vasiee, A., & Tabatabaee Yazdi, F. (2021). Control of microbial growth and lipid oxidation in beef using a Lepidium perfoliatum seed mucilage edible coating incorporated with chicory essential oil. Food Science & Nutrition, 9(5), 2458-2467.
[10] Heydari, S., Jooyandeh, H., Alizadeh Behbahani, B., & Noshad, M. (2020). The impact of Qodume Shirazi seed mucilage‐based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study. Food Science & Nutrition, 8(12), 6497-6512.
[11] Tanavar, H., Barzegar, H., Alizadeh Behbahani, B., & Mehrnia, M. A. (2021). Investigation of the chemical properties of Mentha pulegium essential oil and its application in Ocimum basilicum seed mucilage edible coating for extending the quality and shelf life of veal stored in refrigerator (4°C). Food Science & Nutrition, 9(10), 5600-5615. https://doi.org/https://doi.org/10.1002/fsn3.2522
[12] Yazdi, F. T., & Behbahani, B. A. (2013). Antimicrobial effect of the aqueous and ethanolic Teucrium polium L. extracts on gram positive and gram negative bacteria “in vitro”. Archives of Advances in Biosciences, 4(4), 56-62.
[13] Jalil Sarghaleh, S., Alizadeh Behbahani, B., Hojjati, M., Vasiee, A., & Noshad, M. (2023). Evaluation of the constituent compounds, antioxidant, anticancer, and antimicrobial potential of Prangos ferulacea plant extract and its effect on Listeria monocytogenes virulence gene expression. Front Microbiol, 14, 1202228. https://doi.org/10.3389/fmicb.2023.1202228
[14] Tohidi, B., Rahimmalek, M., & Arzani, A. (2017). Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of Thymus species collected from different regions of Iran. Food Chemistry, 220, 153-161. https://doi.org/https://doi.org/10.1016/j.foodchem.2016.09.203
[15] Hassanpouraghdam, M.-B., Tabatabaie, S.-J., Nazemiyeh, H., Vojodi, L., Aazami, M.-A., & Shoja, A. M. (2008). Chrysanthemum balsamita (L.) Baill.: a forgotten medicinal plant. Facta Universitatis, Medicine and Biology, 15(3), 119-124.
[16] Ivashchenko, I. (2017). Antimicrobial properties of Tanacetum balsamita L.(Asteraceae) introduced in Ukrainian Polissya. Ukrainian Journal of Ecology, 7(1), 52-57.
[17] Bagci, E., Kursat, M., Kocak, A., & Gur, S. (2008). Composition and Antimicrobial Activity of the Essential Oils of Tanacetum balsamita L. subsp. balsamita and T. chiliophyllum (Fisch. et Mey.) Schultz Bip. var. chiliophyllum (Asteraceae) from Turkey. Journal of Essential Oil Bearing Plants, 11(5), 476-484. https://doi.org/10.1080/0972060X.2008.10643656 .
[18] Sharif, M., Najafizadeh, P., Asgarpanah, J., & Mousavi, Z. (2020). <i>In vivo</i> analgesic and anti-inflammatory effects of the essential oil from <i>Tanacetum balsamita</i> L. Brazilian Journal of Pharmaceutical Sciences, 56.
[19] Tabatabaei Yazdi, F., Falah, F., Alizadeh Behbahani, B., Vasiee, A., & Mortazavi, A. (2019). Antimicrobial effect of Citrus aurantium essential oil on some food-borne pathogens and its determination of chemical compounds, total phenol content, total flavonoids content and antioxidant potential. Journal of Food Science and Technology, 16(87), 291-304.
[20] Alizadeh Behbahani, B., Falah, F., Lavi Arab, F., Vasiee, M., & Tabatabaee Yazdi, F. (2020). Chemical Composition and Antioxidant, Antimicrobial, and Antiproliferative Activities of <i>Cinnamomum zeylanicum</i> Bark Essential Oil. Evidence-Based Complementary and Alternative Medicine, 2020, 5190603. https://doi.org/10.1155/2020/5190603
[21] Noshad, M., Alizadeh Behbahani, B., Jooyandeh, H., Rahmati-Joneidabad, M., Hemmati Kaykha, M. E., & Ghodsi Sheikhjan, M. (2021). Utilization of Plantago major seed mucilage containing Citrus limon essential oil as an edible coating to improve shelf-life of buffalo meat under refrigeration conditions. Food Science & Nutrition, 9(3), 1625-1639. https://doi.org/https://doi.org/10.1002/fsn3.2137
[22] Barzegar, H., Alizadeh Behbahani, B., & Mehrnia, M. A. (2020). Quality retention and shelf life extension of fresh beef using Lepidium sativum seed mucilage-based edible coating containing Heracleum lasiopetalum essential oil: an experimental and modeling study. Food Science and Biotechnology, 29(5), 717-728. https://doi.org/10.1007/s10068-019-00715-4
[23] Gevrenova, R., Zengin, G., Sinan, K. I., Zheleva-Dimitrova, D., Balabanova, V., Kolmayer, M., Voynikov, Y., & Joubert, O. (2023). An In-Depth Study of Metabolite Profile and Biological Potential of Tanacetum balsamita L. (Costmary). Plants, 12(1). DOI: 10.3390/plants12010022.
[24] Bączek, K. B., Kosakowska, O., Przybył, J. L., Pióro-Jabrucka, E., Costa, R., Mondello, L., Gniewosz, M., Synowiec, A., & Węglarz, Z. (2017). Antibacterial and antioxidant activity of essential oils and extracts from costmary (Tanacetum balsamita L.) and tansy (Tanacetum vulgare L.). Industrial Crops and Products, 102, 154-163. https://doi.org/https://doi.org/10.1016/j.indcrop.2017.03.009
[25] Gecibesler, I. H., Kocak, A., & Demirtas, I. (2016). Biological activities, phenolic profiles and essential oil components of Tanacetum cilicicum (BOISS.) GRIERSON. Natural Product Research, 30(24), 2850-2855. https://doi.org/10.1080/14786419.2016.1163692
[26] Nooshkam, M., Varidi, M., & Alkobeisi, F. (2022). Bioactive food foams stabilized by licorice extract/whey protein isolate/sodium alginate ternary complexes. Food Hydrocolloids, 126, 107488. https://doi.org/https://doi.org/10.1016/j.foodhyd.2022.107488
[27] Shirani, K., Falah, F., Vasiee, A., Yazdi, F. T., Behbahani, B. A., & Zanganeh, H. (2022). Effects of incorporation of Echinops setifer extract on quality, functionality, and viability of strains in probiotic yogurt. Journal of Food Measurement and Characterization, 16(4), 2899-2907. https://doi.org/10.1007/s11694-022-01399-x .
[28] Bakota, E. L., Winkler-Moser, J. K., Berhow, M. A., Eller, F. J., & Vaughn, S. F. (2015). Antioxidant Activity and Sensory Evaluation of a Rosmarinic Acid-Enriched Extract of Salvia officinalis. Journal of Food Science, 80(4), C711-C717. https://doi.org/https://doi.org/10.1111/1750-3841.12837
[29] Shahidi, F., Tabatabaee Yazdi, F., Nooshkam, M., Zareie, Z., & Fallah, F. (2020). Chemical modification of chitosan through non-enzymatic glycosylation reaction to improve its antimicrobial and anti-oxidative properties. Iranian Food Science and Technology Research Journal 16(1), 117-129. https://doi.org/https://doi.org/10.22067/ifstrj.v16i1.75740 .
[30] Tabatabaei Yazdi, F., Nooshkam, M., Shahidi, F., Asadi, F., & Alizadeh-Behbahani, B. (2018). Evaluation of antimicrobial activity and antioxidant potential of chitosan Maillard-based conjugates in vitro. Applied Microbiology In Food Industries, 4(3), 1-15.
[31] Yousefzadi, M., Ebrahimi, S. N., Sonboli, A., Miraghasi, F., Ghiasi, S., Arman, M., & Mosaffa, N. (2009). Cytotoxicity, Antimicrobial Activity and Composition of Essential Oil from Tanacetum balsamita L. Subsp. Balsamita. Natural Product Communications, 4(1), 1934578X0900400126. https://doi.org/10.1177/1934578X0900400126