Isolation and identification of thermostable amylase producing bacteria from the wastewater of the canning factory

Authors
Soudeh Yousefi fakhr 1
nafiseh davati 2
Dariush Minai-Tehrani3 3
1 MSc Student, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2 Assistant professor, Department of Food Science and Technology, Faculty of Food Industry, Bu-Ali SinaUniversity, Hamedan, Iran.
3 Associate professor, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
10.22034/FSCT.20.139.138
Abstract
The aim of this study was to isolate thermostable amylase producing bacteria from starch-rich wastewater of one of ­the canning factories and then molecular identification of these isolates. In addition, the thermostable amylase extracted from the bacterium was investigated for the optimum temperature and pH of enzyme activity. In this study, 14 heat-resistant microbial isolates were isolated from wastewater and only two isolates had amylase activity. Molecular identification of isolates based on amplification of 16S rDNA gene by B27F and U1492R primers and then sequencing of PCR product confirmed the presence of Bacillus pumilus and Bacillus safensis. The results showed that the optimum temperature and pH of amylase activity were 60°C and 7, ­respectively, and Bacillus safensis 7.67 (U/ml) had more amylase activity than of Bacillus pumilus 6.33 (U/ml).
Keywords
Thermostable amylase
Bacillus
Temperature
Factory wastewater

Subjects

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Journal of food science and technology(Iran)
Volume 20, Issue 139 - Serial Number 139
September 2023
Pages 138-148