Production of edible film based on gelatin containing thyme essential oil: investigation of its physicochemical, mechanical, antioxidant and microbial properties

Authors
1 ilam university
2 Ilam university
Abstract
Considering the tendency to use biodegradable packaging films and increase their antimicrobial ability in recent years, this study aims to investigate different concentrations of thyme essential oil to improve edible film with physicochemical, mechanical, antioxidant and Antimicrobial was done. The effect of different concentrations of thyme essential oil for the production of edible films on the physicochemical, mechanical, antioxidant and antimicrobial properties was investigated with a completely randomized design in three replications and Duncan's multi-range test using Minitab18 software at a probability level of 0.05 were investigated. The highest tensile strength and elongation at break point was 2.5% in the treatment. In the treatment of films, the lowest solubility was observed at a concentration of 3.75% and permeability at a concentration of 2.5% of essential oil. In all treatments, the turbidity was significant, and in the concentration of 3.75% essential oil, the turbidity was the highest, and in the same concentration, the highest thickness was 0.13 mm, which was not significant in all treatments (p<0.05). p). In all edible film treatments, the antioxidant property using DPPH radicals was significant (p<0.05). The results of evaluating the antimicrobial activity of the film use of diffusion disks method showed that the largest diameter of the inhibition halo in the concentration of 3.75% was related to Staphylococcus aureus with an average halo diameter of 15.33 mm. Average halo diameter was reported for Pseudomonas aeruginosa and Escherichia coli, 8.6 mm and 9.52 mm, respectively. The general results showed that the addition of thyme essential oil at a concentration of 2.5% produced films that, in addition to inhibiting the growth and proliferation of bacteria, have sufficient strength and are also capable of being used in perishable food.
Keywords

Subjects


[1] Al-Hassan, A., Norziah, M. 2012. Starch–gelatin edible films: water vapor permeability and mechanical properties as affected by plasticizers. Food Hydrocolloids, 26(1):108-17.
[2] Piermaria, JA., Pinotti, A., Garcia, MA., Abraham, AG.2009. Films based on kefiran, an exopolysaccharide obtained from kefir grain: development and characterization. Food Hydrocolloids, 23(3):684-90.
[3] Sorrentino, A., Gorrasi, G., Vittoria, V.2007. Potential perspectives of bio-nanocomposites for food packaging applications. Trends in Food Science & Technology, 18(2):84-95.
[4] Gennadios, A.2002. Protein-based films and coatings: CRC Press.
[5] Taghizadeh, M., Mohammadifar, MA., Sadeghi, E., Rouhi, M., Mohammadi, R., Askari, F.2018. Photosensitizer-induced cross-linking: A novel approach for improvement of physicochemical and structural properties of gelatin edible films. Food research international, 112:90-7.
[6] Jridi, M., Hajji, S., Ayed, HB., Lassoued, I., Mbarek, A., Kammoun, M.2014. Physical, structural, antioxidant and antimicrobial properties of gelatin–chitosan composite edible films. International journal of biological macromolecule, 67:373-9.
[7] Sahraee, S., Milani, JM., Ghanbarzadeh, B., Hamishehkar, H. 2017. Effect of corn oil on physical, thermal, and antifungal properties of gelatin-based nanocomposite films containing nano chitin. LWT-Food Science and Technology, 76:33-9.
[8] Cheng, S., Wang, W., Li, YGao.G., Zhang, K., Zhou, J.2019. Cross-linking and film-forming properties of transglutaminase-modified collagen fibers tailored by denaturation temperature. Food chemistry, 271:527-35.
[9] Alves, PM., Carvalho, RA., Moraes, IC., Luciano, CG., Bittante, AMQ., Sobral, PJ. 2011. Development of films based on blends of gelatin and poly (vinyl alcohol) cross linked with glutaraldehyde. Food hydrocolloids, 25(7):1751-7.
[10] Chiou, B-S., Avena-Bustillos, RJ., Bechtel, PJ., Jafri, H., Narayan, R., Imam, SH.2008. Cold water fish gelatin films: Effects of cross-linking on thermal, mechanical, barrier, and biodegradation properties. European Polymer Journal, 44(11):3748-53.
[11] Tomadoni, B., Ponce, A., Pereda, M., Ansorena, M.2019. Vanillin as a natural cross-linking agent in chitosan-based films: Optimizing formulation by response surface methodology. Polymer Testing, 10(5):93-95.
[12] Jamróz, E., Juszczak, L., Kucharek, M.2018. Investigation of the physical properties, antioxidant and antimicrobial activity of ternary potato starch-furcellaran-gelatin films incorporated with lavender essential oil. International journal of biological macromolecules. 114:1094-101.
[13] Echeverría, I., López-Caballero, ME., Gómez-Guillén, MC., Mauri, AN., Montero, MP.2018. Active nanocomposite films based on soy proteins-montmorillonite-clove essential oil for the preservation of refrigerated bluefin tuna (Thunnus thynnus) fillets. International journal of food microbiology, 266:142-9.
[14] Ahmed, J., Mulla, M., Arfat, YA., Bher, A., Jacob, H., Auras, R.2018. Compression molded LLDPE films loaded with bimetallic (Ag-Cu) nanoparticles and cinnamon essential oil for chicken meat packaging applications. LWT, 93:329-38.
[15] Bonilla, J., Poloni, T., Lourenço, RV., Sobral, PJ.2018. Antioxidant potential of eugenol and ginger essential oils with gelatin/chitosan films. Food bioscience. 23:107-14.
[16] Tongnuanchan, P., Benjakul, S., Prodpran, T., Pisuchpen, S., Osako, K.2016. Mechanical, thermal and heat sealing properties of fish skin gelatin film containing palm oil and basil essential oil with different surfactants. Food Hydrocolloids. 56:93-107.
[17] Prakash, B., Mishra, PK., Kedia, A., Dubey, N.2014. Antifungal, antiaflatoxin and antioxidant potential of chemically characterized Boswellia carterii Birdw essential oil and its in vivo practical applicability in preservation of Piper nigrum L. fruits. LWT-Food Science and Technology, 56(2):240-7.
[18] Bonilla, J., Poloni, T., Lourenço, RV., Sobral, PJ.2018. Antioxidant potential of eugenol and ginger essential oils with gelatin/chitosan films. Food bioscience. 23:107-14.
[19] Shahbasi, Y., Mosavi, MH. 2016. Study on the effect of Ziziphora clinopodioides essential oil and methanolic pomegranate peel extract on physical, mechanical and antibacterial properties of edible chitosan film. Food technology research journal. 27(2): 121-134.
[20] Ejaz, M., Arfat, YA., Mulla, M., Ahmed, J.2018. Zinc oxide nanorods/clove essential oil incorporated Type B gelatin composite films and its applicability for shrimp packaging. Food Packaging and Shelf Life, 15:113-21.
[21] Han, JH 2003. Antimicrobial food packaging. Novel food packaging techniques, 8:50-70.
[22] Wu, J., Sun, X., Guo, X Ge S., Zhang, Q.2017. Physicochemical properties, antimicrobial activity and oil release of fish gelatin films incorporated with cinnamon essential oil, Aquaculture and Fisheries. 2(4):185-92.
[23] Langroodi ,AM., Hossein, Tajik. 2017. Antimicrobial effects of hydroalchol sumac extract with chitosan containing zataria multiflora boiss essential oil on beef meat in normal and modified atmosphere packaging. The Journal of Urmia University of Medical Sciences, 28(3):192-205
[24] European Commission Regulation No 1129/2011 of 11 November 2011 amending Annex II to Regulation (EC) No 1333/2008 of the European Parliament and of the Council by establishing a :union: list of food additives Text with EEA relevance.
[25] Fallah, M., Rouhi, M., Sadeghi, E., Sarlak, Z., Moghadam, A & Mohammadi, R. 2021. Effects of Olibanum essantial oil on physicochemical, structural, antioxidant and microbial characteristics of Gelatin edible films. Iranian journal of nutrition science and food technology, 15(4): 93-102.
[26] Zafarani, M., Ojagh, SM., Alishahi, A., Kazemi, M. 2017. The effect of chitosan type on the properties of biodegradable films produced from gelatin obtained from the skin of elephant fish. Quarterly journal of new food technologies, 6(1): 149-159.
[27] Hosseini, SF., Rezaei, M. 2016. Development of flexible bactericidal films based on poly (lactic acid) and essential oil and its effectiveness to reduce microbial growth of refrigerated rainbow trout. LWT-Food Science and Technology, 72:251-60.
[28] Ahmad, M., Benjakul, S., Prodpran, T., Agustini, TW.2012. Physico-mechanical and antimicrobial properties of gelatin film from the skin of unicorn leatherjacket incorporated with essential oils. Food Hydrocolloids, 28(1):189-99.
[29] Hanani, ZN., Roos, YH., Kerry, J.2014. Use and application of gelatin as potential biodegradable packaging materials for food products. International journal of biological macromolecules, 71:94-102.
[30] Martucci, JF., Gende, LB., Neira, L., Ruseckaite, R.A.2015. Oregano and lavender essential oils as antioxidant and antimicrobial additives of biogenic gelatin films. Industrial Crops and Products, 71:205-13.
[31] Alexandre, EMC., Lourenço, RV., Bittante, AMQB., Moraes, ICF. 2016. Gelatin-based films reinforced with montmorillonite and activated with nanoemulsion of ginger essential oil for food packaging applications. Food Packaging and Shelf Life, 10:87-96.
[32] Mothana, RA., Hasson, SS., Schultze W., Mowitz, A., Lindequist, U.2011. Phytochemical composition and in vitro antimicrobial and antioxidant activities of essential oils of three endemic Soqotraen Boswellia species. Food chemistry, 126(3):1149-54.
[33] Akhter, R., Masoodi, F., Wani, TA., Rather, SA. 2019. Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents. International journal of biological macromolecules, 137:1245-55.