Biodegradable film of Sodium alginate film/flax seed mucilage/norbixin/tungsten oxide: investigation of color, crystalline, thermal, mechanical and antibacterial properties

Authors
1 Mamaghan Branch, Islamic Azad University, Mamaghan, Iran
2 Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
3 Department of Food hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Abstract
In this research, flax seed mucilage was extracted. Composite film of sodium alginate and flax seed mucilage was prepared. Norbixin pigment and tungsten oxide (WO3) nanoparticles were used to modify the film structure. The color, crystallite, thermal and mechanical properties of the films were investigated. Also, the antibacterial properties of the prepared films against Escherichia coli and Staphylococcus aureus bacteria were investigated. The obtained results showed that the pure alginate/mucilage film does not have very high transparency, which is reduced by adding tungsten oxide nanoparticles and norbixin pigment. The effect of tungsten oxide nanoparticles in reducing film transparency has been greater than that of Norbixin. Examining the factor a (green-red) shows that this factor has increased with the increase of Norbixin and tungsten oxide nanoparticles. Examining factor b (blue-yellow) shows that with the increase of Norbixin and tungsten oxide nanoparticles, this factor has increased. By examining the XRD spectrum of the pure alginate/mucilage film, it was found that this film showed two broad peaks at 2θ of 10° and 20°, which indicates the relatively amorphous structure of this film. In the alginate/mucilage film modified with tungsten oxide nanoparticles, the peaks related to the crystalline nanoparticles in 2θ of approximately 25, 30, 35, 40, 50, 55 and 65 degrees are quite clear, which shows that these nanoparticles improve the crystalline structure of the film. By examining the TGA curves of the films, it was found that the addition of tungsten oxide nanoparticles and norbixin pigment increased the thermal stability of the film. Examining the antibacterial property of the films showed that the addition of tungsten oxide nanoparticles and norbixin pigment increased the antibacterial property of the film significantly (p<0.05).
Keywords

Subjects


[1] Jabraili, A., Pirsa, S., Pirouzifard, M.K. and Amiri, S., 2021. Biodegradable nanocomposite film based on gluten/silica/calcium chloride: physicochemical properties and bioactive compounds extraction capacity. Journal of Polymers and the Environment, 29(8), pp.2557-2571.
[2] Hosseini, S.N., Pirsa, S. and Farzi, J., 2021. Biodegradable nano composite film based on modified starch-albumin/MgO; antibacterial, antioxidant and structural properties. Polymer Testing, 97, p.107182.
[3] Pirsa, S. and Asadi, S., 2021. Innovative smart and biodegradable packaging for margarine based on a nano composite polylactic acid/lycopene film. Food Additives & Contaminants: Part A, 38(5), pp.856-869.
[4] Acik, G., 2020. Preparation of antimicrobial and biodegradable hybrid soybean oil and poly (ʟ-lactide) based polymer with quaternized ammonium salt. Polymer Degradation and Stability, 181, p.109317.
[5] Jiménez‐Rosado, M., Perez‐Puyana, V., Rubio‐Valle, J.F., Guerrero, A. and Romero, A., 2020. Processing of biodegradable and multifunctional protein‐based polymer materials for the potential controlled release of zinc and water in horticulture. Journal of Applied Polymer Science, 137(46), p.49419.
[6] Pirsa, S. and Aghbolagh Sharifi, K., 2020. A review of the applications of bioproteins in the preparation of biodegradable films and polymers. Journal of Chemistry Letters, 1(2), pp.47-58.
[7] Sani, I.K., Geshlaghi, S.P., Pirsa, S. and Asdagh, A., 2021. Composite film based on potato starch/apple peel pectin/ZrO2 nanoparticles/microencapsulated Zataria multiflora essential oil; investigation of physicochemical properties and use in quail meat packaging. Food Hydrocolloids, 117, p.106719.
[8] Asdagh, A., Karimi Sani, I., Pirsa, S., Amiri, S., Shariatifar, N., Eghbaljoo–Gharehgheshlaghi, H., Shabahang, Z. and Taniyan, A., 2021. Production and characterization of nanocomposite film based on whey protein isolated/copper oxide nanoparticles containing coconut essential oil and paprika extract. Journal of Polymers and the Environment, 29(1), pp.335-349.
[9] Sharifi, K.A. and Pirsa, S., 2021. Biodegradable film of black mulberry pulp pectin/chlorophyll of black mulberry leaf encapsulated with carboxymethylcellulose/silica nanoparticles: Investigation of physicochemical and antimicrobial properties. Materials Chemistry and Physics, 267, p.124580.
[10] Fabre, J.F., Lacroux, E., Valentin, R. and Mouloungui, Z., 2015. Ultrasonication as a highly efficient method of flaxseed mucilage extraction. Industrial Crops and Products, 65, pp.354-360.
[11] Roulard, R., Petit, E., Mesnard, F. and Rhazi, L., 2016. Molecular investigations of flaxseed mucilage polysaccharides. International journal of biological macromolecules, 86, pp.840-847.
[12] Pirsa, S., Dalili, R. and Yazdani, I., 2018. Effects of Quince Seed Mucilage and Guar Gum on the Physicochemical and Sensory Properties of Doogh. Journal of Agricultural Science and Technology, 20(3), pp.485-494.
[13] Daei, S., Mohtarami, F. and Pirsa, S., 2022. A biodegradable film based on carrageenan gum/Plantago psyllium mucilage/red beet extract: physicochemical properties, biodegradability and water absorption kinetic. Polymer Bulletin, pp.1-22.
[14] Ye, J., Ma, D., Qin, W. and Liu, Y., 2018. Physical and antibacterial properties of sodium alginate—sodium carboxymethylcellulose films containing Lactococcus lactis. Molecules, 23(10), p.2645.
[15] Hecht, H. and Srebnik, S., 2016. Structural characterization of sodium alginate and calcium alginate. Biomacromolecules, 17(6), pp.2160-2167.
[16] Shabkhiz, M.A., Pirouzifard, M.K., Pirsa, S. and Mahdavinia, G.R., 2021. Alginate hydrogel beads containing Thymus daenensis essential oils/Glycyrrhizic acid loaded in β-cyclodextrin. Investigation of structural, antioxidant/antimicrobial properties and release assessment. Journal of Molecular Liquids, 344, p.117738.
[17] Scotter, M., 2009. The chemistry and analysis of annatto food colouring: a review. Food Additives and Contaminants, 26(8), pp.1123-1145.
[18] Mahendranath, G., Venugopalan, A., Parimalan, R., Giridhar, P. and Ravishankar, G.A., 2011. Annatto pigment production in root cultures of Achiote (Bixa orellana L.). Plant Cell, Tissue and Organ Culture (PCTOC), 106(3), pp.517-522.
[19] Duan, G., Chen, L., Jing, Z., De Luna, P., Wen, L., Zhang, L., Zhao, L., Xu, J., Li, Z., Yang, Z. and Zhou, R., 2019. Robust antibacterial activity of tungsten oxide (WO3-X) nanodots. Chemical research in toxicology, 32(7), pp.1357-1366.
[20] Wang, Q., Wang, H., Zhang, T., Hu, Z., Xia, L., Li, L., Chen, J. and Jiang, S., 2021. Antibacterial Activity of Polyvinyl Alcohol/WO3 Films Assisted by Near-Infrared Light and Its Application in Freshness Monitoring. Journal of Agricultural and Food Chemistry, 69(3), pp.1068-1078.
[21] Priyadarshi, R., Kim, H.J. and Rhim, J.W., 2021. Effect of sulfur nanoparticles on properties of alginate-based films for active food packaging applications. Food Hydrocolloids, 110, p.106155.
[22] Prado, N.S., Silva, I.S.V.D., Silva, T.A.L., Oliveira, W.J.D., Motta, L.A.D.C., Pasquini, D. and Otaguro, H., 2018. Nanocomposite films based on flaxseed gum and cellulose nanocrystals. Materials Research, 21.
[23] Bhagyaraj, S. and Krupa, I., 2020. Alginate-mediated synthesis of hetero-shaped silver nanoparticles and their hydrogen peroxide sensing ability. Molecules, 25(3), p.435.
[24] Perotti, G.F., Silva, F.F., de Couto, R.A., Lima, F.C., Petrilli, H.M., Leroux, F., Ferreira, A. and Constantino, V.R., 2020. Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides. Journal of the Brazilian Chemical Society, 31, pp.2211-2223.
[25] Pang, H.F., Xiang, X., Li, Z.J., Fu, Y.Q. and Zu, X.T., 2012. Hydrothermal synthesis and optical properties of hexagonal tungsten oxide nanocrystals assisted by ammonium tartrate. physica status solidi (a), 209(3), pp.537-544.
[26] Salisu, A., Sanagi, M.M., Abu Naim, A., Abd Karim, K.J., Wan Ibrahim, W.A. and Abdulganiyu, U., 2016. Alginate graft polyacrylonitrile beads for the removal of lead from aqueous solutions. Polymer Bulletin, 73(2), pp.519-537.
[27] Teoh, L.G., Shieh, J., Lau, W.H. and Hon, M.H., 2004. Effects of mesoporous structure on grain growth of nanostructured tungsten oxide. Journal of materials research, 19(9), pp.2687-2693.
[28] Pereira, R., Tojeira, A., Vaz, D.C., Mendes, A. and Bártolo, P., 2011. Preparation and characterization of films based on alginate and aloe vera. International Journal of Polymer Analysis and Characterization, 16(7), pp.449-464.
[29] Handayani, I., Haryanti, P. and Sulistyo, S.B., 2021. Color and antibacterial activity of annatto extracts at various pH of distilled water solvent and extraction temperature. Food Research, 5(6), pp.247-253.
[30] Viuda‐Martos, M., Ciro‐Gómez, G.L., Ruiz‐Navajas, Y., Zapata‐Montoya, J.E., Sendra, E., Pérez‐Álvarez, J.A. and Fernández‐López, J., 2012. In vitro Antioxidant and Antibacterial Activities of Extracts from Annatto (B ixa orellana L.) Leaves and Seeds. Journal of Food Safety, 32(4), pp.399-406.