Evaluation of ultraviolet and visible radiation on Staphylococcus aureus, Pseudomonas aeruginosa of culture medium and total count of oral microbes and solid surfaces

Authors
Mohammad Goli 1
Sharifeh shahi 2
Mahdi Moghzi 3
Rahman Amini 3
Mitra Mansori beni 1
Mohammad Darvishi 2
Seyed Mohsen Ranjbaran 2
1 Department of Food Science and Technology, Faculty of Agriculture, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
2 Laser and Biophotonic in Biotechnologies Research Center, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
3 Department of Medical Engineering, Faculty of Engineering, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
10.22034/FSCT.19.126.333
Abstract
Non-ionizing ultraviolet (UV) radiation is a kind of electromagnetic radiation that has been shown to be harmful to microorganisms such as bacteria, viruses, and fungus. The goal of this study is to evaluate the effective and hazardous features of this type of radiation in three regions (UVA, UVB, and UVC), using the approach of biological properties and applications in disinfection and sterilizing. In this research study, in order to be effective on microorganisms in the ultraviolet spectrum and its surroundings near the visible area, two test samples with commercial UVC and blue-LED lamps were used. In this study, the effect of ultraviolet C radiation on pathogenic microorganisms of Staphylococcus aureus and Pseudomonas aeruginosa in liquid environment and solid surface culture, the effect of ultraviolet C radiation on the total count (total count) of microorganisms on paper and mobile surfaces, and finally to The effect of UV LED on the total number of oral microbes attached to the toothbrush after brushing was investigated. In two tests performed with radiation in the ultraviolet region and near it in the visible region, the lethal effect on bacteria and microorganisms was concluded with more than 90% effectiveness and destruction of bacteria. Which indicates the effectiveness of such radiation in disinfecting, disinfecting and sterilizing equipment. Spectroscopic investigations of UV commercial lamps, as well as their efficacy on microbes, demonstrate the use of this sort of radiation, in addition to its biological hazards, which necessitate careful consideration of how it is utilized. In comparison to alternative sterilizing methods, the use of UV radiation in business and medicine is a cool, dry, easy, effective, and economical technique that produces no ionized radiation.
Keywords
UV radiation
Visible blue-LED light
Pathogenicity
Oral bacterial count
Solid surfaces
Culture medium

Subjects

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