The Effects of High-fat Diet and lactobacillus reuteri on Body Weight, Metabolic profiles and Gut Microbiota of C57BL/6 Mice

Authors
Ehsan Shad 1
Seyed shahram Shekarforoush 2
nazifi s nazifi 3
Mohammad hadi Eskandari 1
1 Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, Iran
2 Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3 Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
10.22034/FSCT.19.126.269
Abstract
This study investigated the effects of diet and probiotic on the body characteristics of C57BL/6 mice. Mice were divided into four groups: the control group, Lactobacillus reutrei DMC20016 probiotic group, high-fat diet (HFD) group, and probiotic with the high-fat diet group. After eight weeks of storage, a high-fat diet and probiotic effects on gut microbiota, body weight, blood factors, leptin hormone, and lipopolysaccharide were studied. A high-fat diet has increased body weight, fat mass, and liver weight. The HFD group had the highest body weight gain (8.36 ± 1.02 gr) compared to the other groups, and consumption of Lactobacillus reuteri did not show a significant effect on body weight. The high-fat diet also significantly increased lipopolysaccharide and leptin, but Lactobacillus reuteri decreased these parameters compared to the HFD group. The abundance and diversity of gut microbiota depended on diet and probiotics consumed. With the consumption of a high-fat diet, the number of Firmicutes ( 70%) increased and Bacteroidetes (<1%) decreased. However, the amount of Actinobacteria (4%) and Firmicutes (16%) decreased, and the amount of Proteobacteria (78%) increased in the H.LR group compared to the control sample. According to this study and similar research, not all probiotics are effective on obesity indicators, and probiotic supplements should be selected based on the purpose of use.
Keywords
Gut Microbiota
Lactobacillus reuteri
Obesity
Probiotic
Weight Change

Subjects

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